ABSTRACT:
Lipase (triacylglycerol acyl hydrolase) hydrolases the triglycerides to fatty acids and glycerol and have the capacity of catalyzes the reverse reaction forming glycerides from glycerol and fatty acids. These are the group of water-soluble enzymes, which exhibit the ability of acting at the interface between aqueous and organic phases. These enzymes find numerous applications in the industrial sector to provide regio selective molecules of commercial interest. Enterobacter is a genus of a common gram-negative, facultative anaerobic, rod-shaped, non-spore-forming bacteria belonging to the family Enterobacteriaceae. Bacteria was isolated from wet soil through serial dilution method and grown on Luria Broth medium. The pure culture was isolated through streak plating. The bacterium was characterized and presumed to be Enterobacter after several biochemical tests. The seed culture was prepared using the isolated pure bacterium. 10% of seed culture is inoculated in the production medium containing olive oil (which acts as an inducer for lipase). Quantitative assay of an enzyme is as to obtain its enzymatic activity, was performed through titrimetric analysis. The activity of crude enzyme was identified as 250 units/ml. The partial purification of crude enzyme was performed using ammonium sulphate. The partially purified enzyme found to have 380 units/ml after dialysis at 4°C.