ABSTRACT:
Because of their chemopreventive action, herbal constituents have got some attention and effectiveness in cost to reduce cancer. Cancer is one of the leading causes of death worldwide. Prostate cancer has become the second highest cause of cancer death and most common malignancy in Western world. The aim of present work is to find out the invitro cell line studies the polyherbal extract constituents on on LN-CAP human Prostate cancer cells. Here 3 drug extracts-vitis viniferae-Grape seed(GSE), Ixora coccinea flower and Piper longum root extract were investigated for their anti-cancer effects against prostate cancer treatment. LN-CAP cells were cultured in DMEM supplemented with1% penicillin, 10% inactivated Fetal Bovine Serum (FBS), humidified atmospheric condition of 5% CO2 at 37°C. By constructing a dose-response curve the IC50 of a drug can be determined. The percentage inhibition of, Piper longum (10–640 µg/ml), vitis viniferae (10–640 µg/ml)and Ixora coccinea (10–640 µg/ml) treatment of cells resulted in 10–58%, 3-53% and 1-55% growth inhibition respectively. In a concentration and time-dependent manner, cell proliferation decreased. In conclusion, results showed that all drug extracts exhibits cytotoxic effects to LN-CAP cells and it could be a potential anticancer drug against prostate cancer cells. Further studies are needed to carry out to confirm the action of drug extracts.