ABSTRACT:
Baccaurea motleyana Müll. Arg. (known locally as Rambai), as an edible fruit plant, is one of the plants native to tropical rain forest of East Kalimantan. This plant is spread in several Asian regions such as Malaysia, Thailand and Indonesia. Traditionally Rambai is used to treat stomach and eye diseases and this plant has anticancer potential. The purpose of this study was to conduct initial screening for bioactivity by the Brine Shrimp Lethality Test (BSLT) method and to determine chemical composition of the most active fraction from the extracts of Rambai woods by the GC-MS analysis. The toxicity test against Artemia salina larvae showed that n-hexane, ethyl acetate and methanol fraction has LC50 values of 39.62, 11.29 and 661.39 ppm, respectively. Furthermore, the acetate fraction as the most active fraction was further fractionated using flash column chromatography and eight fractions were obtained E1, E2, E3, E4, E5, E6, E7, and E8 with LC50 values of 1000 >, 1000 >, 159.52, 138.10, 80.36, 46.06, 47.96 and 72.49 ppm, respectively. The results of GC-MS spectrum characterization of E6 (the most active fraction) showed the presence of alkanes (45.57%), alkenes (27.02%), aromatic compounds (20.90%), fatty acid/fatty acid esters (5.71%), and alcohol (0.78%). Eugenol, 4-ethyl-2-methoxy-phenol, and di-n-octyl phthalate are aromatic compounds that have potential as anticancer drugs.
Cite this article:
Erwin, Zenthise Gandi Tonapa, Alimuddin. Toxicity Assay of Baccaurea motleyana Mull. Arg. Wood Extracts (Rambai) and Chemical Compounds Evaluation for the Most Active Fraction. Research J. Pharm. and Tech. 2020; 13(11):5215-5218. doi: 10.5958/0974-360X.2020.00912.9
Cite(Electronic):
Erwin, Zenthise Gandi Tonapa, Alimuddin. Toxicity Assay of Baccaurea motleyana Mull. Arg. Wood Extracts (Rambai) and Chemical Compounds Evaluation for the Most Active Fraction. Research J. Pharm. and Tech. 2020; 13(11):5215-5218. doi: 10.5958/0974-360X.2020.00912.9 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2020-13-11-27
REFERENCES:
1. Amin R, Nabi MN. Evaluation of Cytotoxic and Antioxidant Activity of Different Fractions of Methanolic Extract of Baccaurea ramiflora (Lour) Fruits, International Current Pharmaceutical Journal, 2015; 4: 386–389.
2. Uddin Md S, Asaduzzaman Md., Al Mamun A, Iqbal MA, Wahid F, Rony RK. Phytochemical analysis and antioxidant profile of methanolic extract of seed, pulp and peel of Baccaurea ramiflora Lour, Asian Pacific Journal of Tropical Medicine, 2018; 11(7): 443-450.
3. Haegens R. Taxonomy, phylogeny, and biogeography of Baccaurea, Distichirhops, and Nothobaccaurea (Euphorbiaceae). Blumea Supplement; 2000. 12(1): 1-218
4. Ismail M, Bagalkotkar, Iqbal S, Adamu HA. Anticancer Properties and Phenolic Contents of Sequentially Prepared Extracts from Different Parts of Selected Medicinal Plants Indigenous to Malaysia, Molecules; 2012, 17(5): 5745–5756
5. Iqbal M, Septina AD. Utilization of Non-Timber Forest Products (NTFPs) by Local People in Sanggau Regency, West Kalimantan, Jurnal Penelitian Ekosistem Dipterokarpa; 2018, 4 (1): 19‐34.
6. Dasman Y, Oramahi, Sisillia, L. Source Food Plants that used By Community Forest Tembawang Village Nanga Kompi Melawi District, Jurnal Hutan Lestari; 2015, 3 (2): 332 – 336.
7. Khoo HE., Azlan A., Kong KW, Ismail A. Review Article: Phytochemicals and Medicinal Properties of Indigenous Tropical Fruits with Potential for Commercial Development, Evidence-Based Complementary and Alternative Medicine; 2016:1-20.
8. Meyer BN, Ferrigni NR Putnam JE, Jacobsen, LB, Nichols DE, McLaughlin JL. Brine Shrimp: A Convenient General Bioassay for Active Plant Constituents", Journal of Medicinal Plants Research, 1982. 45, pp. 31–34.
9. McLaughlin JL. 1991, Bench-top bioassays for the discovery of bioactive compounds in higher plants. Brenesia; 34:1 – 14.
10. Karolina A, Pratiwi DR, Erwin. Phytochemical and Toxicity Test of Merung Extracts (Coptosapelta tomentosa (Blume), Jurnal Atomik; 2018, 03(2) :79-82
11. Supomo1, Syamsul, E. S., Apriliana, A., Saleh, C., Erwin and Lestari, D., Rasayan J. Chem.; 2019, 12(3); 1340-1346
12. Owen RW, Giacosa A., Hull WE, Haubner R. Spiegelhalder B, Bartscha H. The antioxidant/anticancer potential of phenolic compounds isolated from olive oil, European Journal of Cancer; 2000, 36 :1235-1247
13. Ghasemzadeh A, Jaafar, H. ZE, Profiling of phenolic compounds and their antioxidant and anticancer activities in pandan (Pandanus amaryllifolius Roxb.) extracts from different locations of Malaysia, BMC Complementary and Alternative Medicine; 2013, 13 (341): 1-9
14. Galati G, O’brien, PJ. Serial Review: Flavonoids and Isoflavones (Phytoestrogens): Absorption, Metabolism, and Bioactivit, Free Radical Biology and Medicine; 2004, 37 (3): 287 – 303
15. Khalil AA, Rahman U, Khan MR, Sahar A, Mehmoodac T, Khan M, Essential oil eugenol: sources, extraction techniques and nutraceutical perspectives, RSC Adv. ;2017, 7: 32669–32681.
16. Dervis E, Kicar AY, Medine EI, Tekin V, Cosci B., Uygur, E, Muftuler, FZB. In Vitro Incorporation of Radioiodinated Eugenol on Adenocarcinoma Cell Lines (Caco2, MCF7 and PC3), Cancer Biotherapy and Radiopharmaceuticals; 2017, 32 (3), 1-7.
17. Lee, M.H., Yeon, K.Y., Park, C.K., Li, H.Y., Fang, Z., Kim, M.S., Choi, S.Y., Lee, S.J., S. Lee, Park, K., Lee, J.H., Kim .J.S. and Oh, S.B. Eugenol Inhibits Calcium Currents in Dental Afferent Neurons, J Dent Res, 2005; 84 (9): 848-85.
18. Jayashree T, Subramanyam, Antiaflatoxigenic activity of eugenol is due to inhibition of lipid peroxidation, Lett Appl Microbiol.; 1999; 28(3): 179-83.
19. Gaysinsky S, Davidson PM, Barry D, Bruce BD, Weiss, J. Growth Inhibition of Escherichia coli O157:H7 and Listeria monocytogenes by Carvacrol and Eugenol Encapsulated in Surfactant Micelles, Journal of Food Protection; 2005, 68 (12): 2559–2566.
20. Rizvi SMD, Shakil S, Zeeshan M, Khan MS, Shaikh S, Biswas D, Ahmad A, Kamal MA. An Enzoinformatics Study Targeting Polo-Like Kinases-1-Enzyme: Comparative Assassment of Anticancer Potential of Compounds Isolated Leaves of Ageratum houstonianum, Pharmacognosy Magazine; 20014, 10: 973-1296
21. Kim MK, Nam PW, Lee SJ, Lee, KG. Antioxidant activities of volatile and non‐volatile fractions of selected traditionally brewed Korean rice wines, J. Inst. Brew; 2014, 120: 537–542
22. Hase GJ, Deshmukh KK, Pokharkar RD, Gaje TR, Phatanagre ND. Phytochemical Studies on Nerium oleander L. Using GC-MS, International Journal of Pharmacognosy and Phytochemical Research; 2017), 9 (6): 885-891.
23. Rajeswari J, Rani S. GC-Ms Analysis of Whole Plant of Leptadenia Reticulata, Int. J. Pharm Tech Res.; 2014, 6(7): 2043-2050.
24. Dr. Duke's Phytochemical and Ethnobotanical Databases. Available from: URL: https://phytochem.nal.usda.gov/phytochem/search/list.