Author(s): Nur Illiyyin Akib, Cici Novianti, Halimahtussaddiyah Ritonga, Suryani, Andi Nafisah Tendri Adjeng

Email(s): nafisahandi23@gmail.com

DOI: 10.5958/0974-360X.2020.00918.X   

Address: Nur Illiyyin Akib1, Cici Novianti1, Halimahtussaddiyah Ritonga2, Suryani1, Andi Nafisah Tendri Adjeng1*
1Department of Pharmacy, Faculty of Pharmacy, Universitas Halu Oleo, Jl. HEA. Mokodompit, Anduonohu, Kendari-South East Sulawesi, Indonesia, 93232.
2Mathematics and Natural Science, Universitas Halu Oleo, Jl. HEA. Mokodompit, Anduonohu, Kendari-South East Sulawesi, Indonesia, 93232.
*Corresponding Author

Published In:   Volume - 13,      Issue - 11,     Year - 2020


ABSTRACT:
Kojic acid is a metabolite of Acetobacter, Asperigillus, and Penicillium fungi species that can prevent skin hyperpigmentation. Kojic acid is prepared in ethosome vesicles to increase its penetration into the skin and reduce the irritant effect. The aim of this research was to obtain the optimum content of kojic acid in ethosome vesicles. Methods: Kojic acid at a concentration of 1% was prepared in ethosome vesicles by hot (40oC) and cold methods (30oC). Phosphatidylcholine concentrations during vesicle formation were 2% and 3%. Ethanol concentrations as penetration enhancer were 20%, 25%, 30%, 35%, and 40%. Characterizations of vesicles included analysis of shape and size by using optical microscope and efficiency of entrapment was measured by using spectrophotometer at ? max 268nm. Kojic acid content that was optimized was 0.5%, 1%, 1.5%, 2%, and 2.5%. Results: The best formula obtained was phosphatidylcholine 2% and ethanol 40% which were prepared by the hot method. Shape of ethosome vesicles was large unilamellar vesicle, the smallest size was 281 nm and entrapment efficiency were 47.7%. The optimum content of kojic acid was 2% with 429.6 nm for size and entrapment efficiency was 73.0%. Conclusion: We conclude that kojic acid can be used in preparation of ethosome vesicles and future research may use this approach for clinical research.


Cite this article:
Nur Illiyyin Akib, Cici Novianti, Halimahtussaddiyah Ritonga, Suryani, Andi Nafisah Tendri Adjeng. Optimization of Method and Proportion of Phosphatidylcholine and Ethanol for Preparation of Kojic Acid Ethosome. Research J. Pharm. and Tech. 2020; 13(11):5251-5256. doi: 10.5958/0974-360X.2020.00918.X

Cite(Electronic):
Nur Illiyyin Akib, Cici Novianti, Halimahtussaddiyah Ritonga, Suryani, Andi Nafisah Tendri Adjeng. Optimization of Method and Proportion of Phosphatidylcholine and Ethanol for Preparation of Kojic Acid Ethosome. Research J. Pharm. and Tech. 2020; 13(11):5251-5256. doi: 10.5958/0974-360X.2020.00918.X   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2020-13-11-33


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