The present work was carried out to produce pectin lyase and cellulase production form the isolated fungi. The use of microorganisms for the production of enzymes offers a promising approach for its large scale production and as a possible food supplement or in pharmaceutical industry. The pectin lyase and cellulase producing Aspergillus niger was isolated from soil and characterized by staining procedures and partial purification. From the present study, the result showed that pectin lyase and cellulase producing fungi can grow at optimized condition. Thereby, partial purification of pectin lyase and cellulase enzyme was done. The Aspergillus niger showed a potential to convert pectin lyase and cellulase into reducing sugars which could be readily used in many applications such as animal foods and a feed stock for production of valuable organic compounds.
Cite this article:
Ujjwala Supe. Source and application of cellulose and pectin lyase – A review. Research J. Pharm. and Tech. 2020; 13(11):5635-5641. doi: 10.5958/0974-360X.2020.00982.8
Ujjwala Supe. Source and application of cellulose and pectin lyase – A review. Research J. Pharm. and Tech. 2020; 13(11):5635-5641. doi: 10.5958/0974-360X.2020.00982.8 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2020-13-11-97
1. Robinson PK Enzymes: principles and biotechnological applications. Essays in Biochemistry, 2015; 15(59): 1–41.
2. Schnell S, MJ Chappell, ND Evans, M R Roussel The mechanism distinguish ability problem in biochemical kinetics: The single-enzyme, single substrate reaction as a case study, Comptes Rendus Biologies., 2006; 329: 516
3. Becker-Ritt, AB, AHS Martinelli, S Mitidieri, V Feder, GE Wassermann, L Santi, M H Vainstein, JTA Oliveira, LM Fiuza, G Pasquali, CR Carlin (2007) Antifungal activity of plant and bacterial ureases, Toxicon., 2007; 50: 971-983.
4. Gurung N, Ray S, Bose S, Rai V A broader view: microbial enzymes and their relevance in industries, medicine, and beyond Biomed. Res. Int.,2013; 18,https://doi.org/10.1155/2013/329121.
5. Hawlader, M.S. and S. M. Tareeq, Multi-objective approach in predicting amino acid interaction network using ant colony optimization International Journal of Artificial Intelligence & Applications (IJAIA), 2014; 05 (01): 113-126.
6. Yadav, S., Yadav, PK., Yadav, D. and Yadav, KDS, Purification and characterization of an alkaline pectin lyase from Aspergillus flavus. Process Biochem, 2008; 43:547-552
7. Immanuel G, Dhanusha R, Prema P, Palavesam A,Effect of different growth parameters on endoglucanase enzyme activity by bacteria isolated from coir retting effluents of estuarine environment, Int. J. Environ. Sci. Technol., 2006; 3:25-34.
8. Chinedu SN, Okochi V, Smith HA, Okafor UA, Onyegeme-Okerent, BM, Omidiji O Effect of carbon sources on cellulase (EC 3. 2. 1. 4) production by Penicillium chrysogenum PCL501, Afr. J. Biochem Res.,2007; 1(1): 006-010.
9. Szijarto N, Szengyel Z, Liden G, Reczey K et al,. Dynamics of cellulase production by glucose grown cultures of Trichoderma reesei Rut-C30 as a response to addition of cellulose, Applied Biochemistry and Biotechnology, Part A, 2004; 113(1–3) 115–124.
10. Sharada R, Venkateswarlu G, Venkateswar S, Anand Rao M, Applications Of Cellulases : Review. International Journal Of Pharmaceutical, Chemical and Biological Science., 2014; 4: 424-437.
11. Bayer EA, Lamed R, Himmel ME. The potential of cellulases and cellulosomes for cellulosic waste management. Current Opinion in Biotechnology, 2007; 18: 237-245.
12. Hernandez C, Milagres AMF, Vazquez-Marrufo G, Munoz-Paez KM, Garcia-Perez JA, Alarcon E. An ascomycota coculture in batch bioreactor is better than polycultures for cellulase production. Folia Microbiology (Praha). 2018; Doi: https://doi.org/10.1007/s12223-018-0588-1.
13. Cerda A, Gea T, Vargas-García MC, Sánchez A, Towards a competitive solid state fermentation: Cellulases production from coffee husk by sequential batch operation and role of microbial diversity. Science of The Total Environment, 2017; 589, 56-65, Doi: https://doi.org/10.1016/j.scitotenv.2017.02.184.
14. Phitsuwan P, Laohakunjit N, Kerdchoechuen O, Kyu KL, Ratanakhanokchai K, Potential applications of cellulases in Agriculture, Biotechnology and Bioenergy Folia Microbiologica , 2013; 58(2):163-176.
15. Bhat M, Bhat S, Cellulase degrading enzymes and their potential industrial applications. Biotechnology Advances., 1997; 15(3-4):583-620.
16. Kenealy WR, Jeffries TW Enzyme processes for pulp and paper: A review of recent developments,” In: Mansfield, S., Saddler, J. (ed.), Applications of Enzymes to Lignocellulosics. ABS Symposium series 855, American Chemical Society, Chapter 12: 2003; 210-239.
17. Menendez E, Garcia-Fraile P, Rivas R, Biotechnological applications of bacterial cellulases. AIMS Bioengineering, 2015; 2: 163-182.
18. Horn SJ, Vaaje-Kolstad G, Westereng B, Eijsink V,Novel enzymes for the degradation of cellulose. Biotechnology for biofuels, 2012; 5: 45, https://doi.org/10.1186/1754-6834-5-45.
19. Sharada R, Venkateswarlu G, Venkateshwar S, Rao MA Production of cellulase–a review. Int J Pharmaceut Chem Biol Sci., 2013; 3(4):1070-1090.
20. Budihal, S., R., D. Agsar, and S. Patil, R. Enhanced production and application of acido thermophilic Streptomyces cellulase. Bioresource Technology, 2016; 200: 706-712.
21. Przybysz Buzała K, Przybysz P, Kalinowska H, Derkowska M, Effect of cellulases and xylanases on reﬁning pro-cess and kraft pulp properties, PLOS ONE, 2016; 11, e0161575, https://doi.org/10.1371/journal.pone.0161575.
22. Demuner BJ, Pereira Junior N, Antunes AMS, Technology Prospecting on Enzymes for the Pulp and Paper Industry, 2011; 6: 11 https://doi.org/10.4067/S0718-27242011000300011.
23. Kuhad R, C, Gupta R, Singh A, Microbial Cellulases and Their Industrial Applications. Enzyme Research, 2011, PP 10 https://doi.org/10.4061/2011/280696.
24. Gautam SP, Bundela PS, Pandey AK, Khan J, Awasthi MK, Sarsaiya S. Optimization for the Production of Cellulase Enzyme from Municipal Solid Waste Residue by Two Novel Cellulolytic Fungi. Biotechnology Research International, 2011; 8, https://doi.org/10.4061/2011/810425.
25. Murad H, Azzaz H, Cellulase and dairy animal feeding. Biotechnology, 2010; 9: 238-256,
26. Asmare B, Biotechnological Advances for Animal Nutrition and Feed Improvement. World Journal of Agricultural Research, 2014; 2: 115-118,
27. Juturu V, Wu JC, Microbialexo-xylanases: a mini review. Applied Biochemistry and Biotechnology, 2014; 174: 81–92.
28. Arja M, Araujo R, Casal M, CavacoPaulo A, Cellulases in the textile industry Industrial enzymes, 2008; 51-63, Springer. https://doi.org/10.1007/1-4020-5377-0_4.
29. Araujo R, Casal M, Cavaco Paulo A, Application of enzymes for textile fibres processing. Biocatalysis and Biotransformation, 2008; 26, 332-349, Doi.org https://doi.org/10.1080/ 10242420802390457.
30. Behera B, Sethi B, Mishra R, Dutta S, Thatoi H. Microbial cellulases–Diversity & biotechnology with reference to mangrove environment: A review. Journal of Genetic Engineering and Biotechnology., 2016; 15, 197-210.
31. Phitsuwan P, Laohakunjit N, Kerdchoechuen O, Kyu KL, Ratanakhanokchai K. Present and potential applications of cellulases in agriculture, biotechnology, and bioenergy. Folia microbiologica, 2013; 58, 163-176, Doi: https://doi.org/10.1007/ s12223-012-0184-8
32. Zhang Y, Tang B, Du G, Self-induction system for cellulase production by cellobiose produced from glucose in Rhizopus stolonifer. Scientific Report, 2017; 7: 10161,
33. Aliakbarian B, De Faveri D, Casazza A, Oliveira R, Oliveira M, Converti A, Perego P, (2011) Bio-extraction of olive oil: improvement of quality and extraction outputs:. Available at: http://www.aidic.it/IBIC2008/webpapers/103Aliakbarian.pdf.
34. Li S, Yang X, Yang S, Zhu M, Wang X, Technology Prospecting on Enzymes: Application, Marketing and Engineering. Computational and Structural Biotechnology Journal, 2012; 2: e201209017.