Author(s):
Hilda Maysarah, Meutia Faradilla, Irma Sari, Didi Nurhadi Illian
Email(s):
didinurhadi40@gmail.com
DOI:
10.5958/0974-360X.2020.01013.6
Address:
Hilda Maysarah, Meutia Faradilla, Irma Sari, Didi Nurhadi Illian*
Department of Pharmacy, Faculty of Mathematics and Natural Science, Syiah Kuala University, Banda Aceh 23111, Indonesia.
*Corresponding Author
Published In:
Volume - 13,
Issue - 12,
Year - 2020
ABSTRACT:
Rodent tuber (Typhonium flagelliforme (Lood) Bl) has been known and used as a food supplement for an alternative cancer therapy and tumors, including breast tumors. Numerous in vitro studies were exhibited an anticancer activity of this plant compound against several cancer cells, hence the present study as a further in vivo research to determine its cancer chemopreventive activity. The survival rate of animals, latency period of tumors, percentage of tumor incidence, number and size of tumors, weight of tumors, fluorescence intensity of autofluorosphore (FIA) and histological evaluation were investigated. The extracts of T. flagelliforme were obtained by maceration using 95% ethanol and condensed utilize rotary evaporator. The chemopreventive effect of T. flagelliforme was determined by observing the activity of these extract toward female Sprague-Dawley rats induced breast tumor using single dose of 15mg of 7,12-dimethylbenz (a) anthracene (DMBA) per rat (given orally). Rats given only DMBA were used as a control group. T. flagelliforme extract (50mg/kg bw) given for 7 days before and after induction (test group). The results showed that whole animal of chemopreventive group were survived and the group given T. flagelliforme extract (50mg/kg bw) were reduced latency period of tumors at 12 weeks compared to the control group at 9 weeks. The percentage of tumor incidence, number and size of tumors (33.33%; 0.50 and 0.39 cm2) were exhibited significantly different compared to the control group (100%; 2.83 and 2.4cm2), respectively (p < 0.001). The weight of tumors and FIA values were revealed significantly different (0.26g and 6.00) compared to control group (5.34g and 11.62), respectively. Furthermore, histological evaluation was demonstrated that the test group (given T. flagelliforme extract) had a different histopathologic figure and malignancy level compared to the control group. The conclusion exhibited that T. flagelliforme extract can be used as cancer chemopreventive against breast tumor.
Cite this article:
Hilda Maysarah, Meutia Faradilla, Irma Sari, Didi Nurhadi Illian. Cancer Chemopreventive effect of Rodent Tuber (Typhonium flagelliforme (Lood) Bl) against DMBA-Induced Rats Breast Tumor. Research J. Pharm. and Tech. 2020; 13(12):5811-5815. doi: 10.5958/0974-360X.2020.01013.6
Cite(Electronic):
Hilda Maysarah, Meutia Faradilla, Irma Sari, Didi Nurhadi Illian. Cancer Chemopreventive effect of Rodent Tuber (Typhonium flagelliforme (Lood) Bl) against DMBA-Induced Rats Breast Tumor. Research J. Pharm. and Tech. 2020; 13(12):5811-5815. doi: 10.5958/0974-360X.2020.01013.6 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2020-13-12-28
REFERENCES:
1. Corwin EJ. Pocket Book of Pathophysiology. 3rd ed. EGC Medical Book Publishers, Jakarta (2001): 356.
2. American Cancer Society. Breast Cancer Facts & Figures 2017–2018. Atlanta: American Cancer Society, Inc. 2017.
3. Sugunadevi G, Suresh K, Manoharan S, Vijayaanand MA, Rajalingam K. Modifying effects of mosinone-A on glycoconjugates levels in 7,12-dimethyl benz(a)anthracene induced hamster buccal pouch carcinogenesis. Asian J of Res in Chem. 2010; 3(2): 459–463.
4. Davis L and Kultan G. Effect of Withania somnifera on DMBA induced carcinogenesis. J Ethanopharmacol. 2001; 75 :165–168.
5. Illian DN, Hasibuan PAZ, Sumardi S, et al. Anticancer activity of polyisoprenoids from Avicennia alba Blume. in WiDr cells. Irani J of Pharm Res. 2019; 18(3): 1477–1487.
6. Purwaningsih E, Suciati Y, Widayanti E. Anticancer effect of a Typhonium flagelliforme L. in Raji cells through telomerase expression. Indones J Cancer Chemoprevent. (2017); 8(1): 15–20.
7. Mohan S, Bustamam A, Ibrahim S, et al. In vitro ultramorphological assessment of apoptosis on CEMss induced by linoleic acid-rich fraction from Typhonium flagelliforme tuber. Evidence-Based Complementary and Alternetive Medicine. 2011: 1–12.
8. Lai CS, Mas RHMH, Nair NK, et al. Typhonium flagelliforme inhibits cancer cell growth in vitro and induced apoptosis: An evaluation by the bioactivity guided approach. J of Etnopharmacol. 2008; 118(1): 14–20.
9. Lai CS, Mas RHMH, Nair NK, Mansor SM, Navaratnam V. Chemical constituents and in vitro anticancer activity of Typhonium flagelliforme (Araceae). J of Etnopharmacol. 2010; 127(2): 468–494.
10. Purwaningsih E, Widayanti E, Suciati Y. Cytotoxicity assay of Typhonium flagelliforme Lodd against breast and cervical cancer cells. Univ Med. 2014; 33(2): 75–82.
11. Harris RE, Alshafie GA, Abou-Issa H, Seibert K. Chemoprevention of breast cancer in rats by celecoxib, a cyclooxygenase 2 inhibitor. Cancer Res. 2000; 60: 2101–2103.
12. Karthikeyan K, Masilamani V, Govindasamy S. Spectrofluorimetric detection of DMBA-induced mouse skin carcinoma. Pathol Oncol Res. 1999; 5(1): 46–48.
13. Amat S, Penault-Llorca F, Cure H, et al. Scarff-Bloom-Richardson (SBR) grading: a pleiotropic marker of chemosensitivity in invasive ductal breast carcinomas treated by neoadjuvant chemotherapy. Int J of Oncol. 2002; 20: 791–796.
14. Bansal C, Singh US, Misra S, et al. Comparative evaluation of the modified Scarff-Bloom-Richardson grading system on breast carcinoma aspirates and histopathology. Cyto J. 2012; 9(4): 1–5.
15. Nurrochmad A, Lukitaningsih E, Meiyanto E. Anti cancer activity of rodent tuber (Thyphonium flagelliforme (lodd.) Blume on human breast cancer T47D cells. Intl J of Phytomed. 2011; 3: 138–146.
16. Suresh R, Benitojohnson D, Maheswari C, Venkatnarayanan R, Manavalan R. Chemo preventive activity of Triumfetta rhomboidea in 7, 12-dimethylbenz (A) anthracene induced breast cancer in Sprague–Dawley rat model. Research J Pharm and Tech. 2017; 10(3): 687–692.
17. Khalivulla SI, Mohammed A, Sirajudeen KNS, Shaik MI, Ye W, Korivi M. Novel phytochemical constituents and anticancer activities of the genus, Typhonium. Curr Drug Metab. 2019; 20(12): 946–957.
18. Choo CY, Chan KL, Sam TW, Hitotsuyanagi Y, Takeya K. The cytotoxicity and chemical constituents of the hexane fraction of Typhonium flagelliforme (Araceace). J Ethnopharmacol. 2001 Sep; 77(1): 129–31.
19. Kim YS, Li XF, Kang KH, Ryu B, Kim SK. Stigmasterol isolated from marine microalgae Navicula incerta induces apoptosis in human hepatoma hepG2 cells. BMB Rep. 2014; 47: 433–8.
20. Yu FR, Lian XZ, Guo HY, McGuire PM, Li RD, Wang R, et al. Isolation and characterization of methyl esters and derivatives from Euphorbia kansui (Euphorbiaceae) and their inhibitory effects on the human SGC7901 cells. J Pharm Pharm Sci. 2005; 8: 528–35.
21. Paul S, Kundu R. Induction of apoptosis by fatty acid rich fraction of Solanum nigrum on cervical cancer cell lines. Int J Pharm Pharm Sci. 2017; 9: 199–206.
22. Chujo H, Yamasaki M, Nou S, Koyanagi N, Tachibana H, Yamada K, et al. Effect of conjugated linoleic acid isomers on growth factor-induced proliferation of human breast cancer cells. Cancer Lett. 2003; 202: 81–7.
23. Evans LM, Cowey SL, Siegal GP, Hardy RW. Stearate preferentially induces apoptosis in human breast cancer cells. Nutr Cancer. 2009; 61: 746–53.
24. Hardy RW, Wickramasinghe NS, Ke SC, Wells A. Fatty acids and breast cancer cell proliferation. Adv Exp Med Biol. 1997; 422: 57–69.
25. Wickramasinghe NS, Jo H, McDonald JM, Hardy RW. Stearate inhibition of breast cancer cell proliferation. A mechanism involving epidermal growth factor receptor and G-proteins. Am J Pathol. 1996; 148: 987–95.
26. Evans LM, Toline EC, Desmond R, Siegal GP, Hashim AI, Hardy RW, et al. Dietary stearate reduces human breast cancer metastasis burden in athymic nude mice. Clin Exp Metastasis. 2009; 26: 415–24.
27. Iyer VV, Griesgraber GW, Radmer MR, McIntee EJ, Wagner CR. Synthesis, in vitro anti-breast cancer activity, and intracellular decomposition of amino acid methyl ester and alkyl amide phosphoramidate monoesters of 3’-azido-3’-deoxythymidine (AZT). J Med Chem. 2000; 43: 2266–74.
28. Li C, Zhao X, Toline EC, Siegal GP, Evans LM, Ibrahim-Hashim A, et al. Prevention of carcinogenesis and inhibition of breast cancer tumor burden by dietary stearate. Carcinogenesis. 2011; 32: 1251–8.
29. Saadatian-Elahi M, Norat T, Goudable J, Riboli E. Biomarkers of dietary fatty acid intake and the risk of breast cancer: A meta-analysis. Int J Cancer. 2004; 111: 584–91.
30. Sianipar NF, Assidqi K, Purnamaningsih R, Herlina T. In vitro cytotoxic activity of rodent tuber mutant plant (Typhonium flagelliforme Lodd.) against to MCF-7 breast cancer cell line. Asian J Pharm Clin Res. 2019; 12(3): 185–189.