Author(s): P. Jayashree, M. Shalini, Meenambiga S. S., V. Suganya


DOI: 10.5958/0974-360X.2020.01028.8   

Address: P. Jayashree, M. Shalini, Meenambiga S. S.*, V. Suganya
Department of Bio-Engineering, School of Engineering, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Chennai, India.
*Corresponding Author

Published In:   Volume - 13,      Issue - 12,     Year - 2020

The present study was carried out in Epiphyllum oxypetalum flower to analyse the bioactive compounds present in it. Epiphyllum oxypetalum is a unique plant which blooms only in the night month of June to September. The bioactive compounds obtained from medicinal plant have been treated for various ailments caused by micro- organism and it has been analysed with various part of the plant but not in the flower of this plant. Extraction was carried out by using flower sample using maceration method with solvents such as chloroform, ethanol, hexane and aqueous. The crude extracted sample was tested for phytochemical activity, and it showed the presence of compounds such as alkaloids, saponins, proteins and amino acids, terpenoids, steroids, flavonoids, and tannins. Thin layer Chromatography and Column chromatography was performed with the formation of 4 bands in the ethanol extract. The purified sample has been further analysed using Gas chromatography-Mass spectrometry [GC-MS] to check the phytocompounds present in the sample. The first compound identified with less retention time (2.86min) was Hexadecanoic acid, ethyl ester and the last compound which took long retention time (30.81min) was Pterin-6- carboxylic acid. Thus, these plant compounds could be isolated in future to explore their diverse biological activities.

Cite this article:
P. Jayashree, M. Shalini, Meenambiga S. S., V. Suganya. Phytochemical Screening and GC-MS Analysis of Epiphyllum oxypetalum flower extracts. Research J. Pharm. and Tech. 2020; 13(12):5893-5897. doi: 10.5958/0974-360X.2020.01028.8

P. Jayashree, M. Shalini, Meenambiga S. S., V. Suganya. Phytochemical Screening and GC-MS Analysis of Epiphyllum oxypetalum flower extracts. Research J. Pharm. and Tech. 2020; 13(12):5893-5897. doi: 10.5958/0974-360X.2020.01028.8   Available on:

2.    Upendra RS, Khandelwal P. Assessment of nutritive values, phytochemical constituents and biotherapeutic potentials of Epiphyllum oxypetalum. Int. J. Pharm. Pharmaceut. Sci. 2012;4:421-5
3.    Matsuura N. The analysis of the aroma ingredients of the flower of Queen of the Night (Epiphyllum oxypetalum Haw.). Korio. 2002;214:12
4.    Kumar PP, Kumaravel S, Lalitha C. Screening of antioxidant activity, total phenolics and GC-MS study of Vitexnegundo. African Journal of Biochemistry Research. 2010 Jul 31;4(7):191-5.
5.    Ahmed S, Ahmad M, Swami BL, Ikram S. Green synthesis of silver nanoparticles using Azadirachta indica aqueous leaf extract. Journal of Radiation Research and Applied Sciences. 2016 Jan 1;9(1):1-7.
6.    Cheikhyoussef A, Naomab E, Potgieter S, Kahaka GK, Raidron C, Mu Ashekele H. Phytochemical properties of a Namibian indigenous plant; Eembe (Berchemia discolor)
7.    Dandekar R, Fegade B, Bhaskar VH. GC-MS analysis of phytoconstituents in alcohol extract of Epiphyllum oxypetalum leaves. Journal of Pharmacognosy and Phytochemistry. 2015 May 1;4(1).
8.    Handley AJ, Adlard ER, editors. Gas chromatographic techniques and applications. Taylor and Francis; 2001.
9.    Chauhan A, Goyal MK, Chauhan P. GC-MS technique and its analytical applications in science and technology. J Anal Bioanal Tech. 2014 Nov 1;5(6):222.
10.    Soni A, Sosa S. Phytochemical analysis and free radical scavenging potential of herbal and medicinal plant extracts. Journal of Pharmacognosy and Phytochemistry. 2013 Nov 1;2(4):22-9.
11.    Joshi B, Sah GP, Basnet BB, Bhatt MR, Sharma D, Subedi K, Janardhan P, Malla R. Phytochemical extraction and antimicrobial properties of different medicinal plants: Ocimum sanctum (Tulsi), Eugenia caryophyllata (Clove), Achyranthes bidentata (Datiwan) and Azadirachta indica (Neem). Journal of Microbiology and Antimicrobials. 2011 Jan 30;3(1):1-7.
12.    Raphael E. Phytochemical constituents of some leaves extract of Aloe vera and Azadirachta indica plant species. Global Advanced Research Journal of Environmental Science and Toxicology. 2012 May;1(2):014-7.
13.    Yadav RN, Agarwala M. Phytochemical analysis of some medicinal plants. Journal of phytology. 2011 Dec 14.
14.    Sonam M, Singh RP, Pooja S. Phytochemical screening and TLC profiling of various extracts of Reinwardtiaindica.2017 Apr ; 9(4); 523-527
15.    Darvishi MT, Kheybari S, Yildirim A. Application of He’s parameter-expansion method to a system of two van der Pol oscillators coupled via a Bath. Nonlin. Science Lett. A. 2010;1(4):399-405.
16.    Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction: a review. Internationale Pharmaceutica Sciencia. 2011 Mar;1(1):98-106.
17.    Jaradat N, Hussen F, Al Ali A. Preliminary phytochemical screening, quantitative estimation of total flavonoids, total phenols and antioxidant activity of Ephedra alata Decne. J Mater Environ Sci. 2015;6(6):1771-8.
18.    Wiegand I, Hilpert K, Hancock RE. Agar and broth dilution methods to determine the Minimal Inhibitory Concentration (MIC) of antimicrobial substances. Nature Protocols. 2008 Feb;3(2):163.
19.    Kumar ks, Rao AS. Extraction, isolation and structural elucidation of flavonoid from Chrozophoraplicata leaves and evaluation of its antioxidative potentials, Extraction 2017;10(4).
20.    Ahuja S, Alsante KM, editors. Handbook of isolation and characterization of impurities in pharmaceuticals. Academic Press; 2003.
21.    Eloff JN. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Plantamedica. 1998 Dec;64(08):711-3.
22.    Sermakkani M, Thangapandian V. GC-MS analysis of Cassia italica leaf methanol extract. Asian J Pharm Clin Res. 2012;5(2):90-4.
23.    Deshmukh, O.S. GC-MS analysis of root acetone extract of Spatholobuspurpureus- a high ethno-veterinary medicinal value plant. International Journal of Current Research 2015 nov;
24.    Ara I, Shinwari MM, Rashed SA, Bakir MA. Evaluation of Antimicrobial Properties of Two Different Extracts of Juglansregia Tree Bark and Search for Their Compounds Using Gas Chromatography-Mass Spectrum. International Journal of Biology. 2013 Mar 13;5(2):92.
25.    Kumar S, Jyotirmayee K, Sarangi M. Thin layer chromatography: a tool of biotechnology for isolation of bioactive compounds from medicinal plants. International Journal of Pharmaceutical Sciences Review and Research. 2013;18(1):126-32.
26.    Kozima T, Ogisu S, inventors; Aichi Steel Works Ltd, assignee. Lubricant and method for non-chip metal forming. United States patent US 4,168,241. 1979 Sep 18.
27.    Asghar A, Lin CF, Gray JI, Buckley DJ, Booren AM, Crackel RL, Flegal CJ. Influence of oxidised dietary oil and antioxidant supplementation on membrane‐bound lipid stability in broiler meat. British Poultry Science. 1989 Dec 1;30(4):815-23.
28.    Anitha J, Miruthula S. Traditional medicinal uses, phytochemical profile and pharmacological activities of Luffa acutangula Linn. International Journal of Pharmacognosy. 2014;1(3):174-83.
29.    Satpute SK, Banat IM, Dhakephalkar PK, Banpurkar AG, Chopade BA. Biosurfactants, bioemulsifiers and exopolysaccharides from marine microorganisms. Biotechnology Advances. 2010 Jul 1;28(4):436-50.
30.    Al Hashmi LS, Hossain MA, Weli AM, Al-Riyami Q, Al-Sabahi JN. Gas chromatography-mass spectrometry analysis of different organic crude extracts from the local medicinal plant of Thymus vulgaris L. Asian Pacific Journal of Tropical Biomedicine. 2013 Jan;3(1):69.
31.    Mahmud KN, Yahayu M, Sarip SH, Rizan NH, Min CB, Mustafa NF, Mustafa NF, Ngadiran S, Ujang S, Zakaria ZA. Evaluation on Efficiency of Pyroligneous Acid from Palm Kernel Shell as Antifungal and Solid Pineapple Biomass as Antibacterial and Plant Growth Promoter. Sains Malaysiana. 2016 Oct 1;45(10):1423-34.
32.    Lacy A, O'kennedy R. Studies on coumarins and coumarin-related compounds to determine their therapeutic role in the treatment of cancer. Current Pharmaceutical Design. 2004 Nov 1;10(30):3797-811.
33.    Hussein AO, Mohammed GJ, Hadi MY, Hameed IH. Phytochemical screening of methanolic dried galls extract of Quercus infectoria using gas chromatography-mass spectrometry (GC-MS) and Fourier transform-infrared (FT-IR). Journal of Pharmacognosy and Phytotherapy. 2016 Mar 31;8(3):49-59.

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