ABSTRACT:
India is considered as the potential feedstock and richest sources of herbals that have potent medicinal values. The wide distribution of flora in India offers medicinally active plants and their derivatives for treatment of various ailments like heart disease, skin disorders and various cancers. Jasminium pubescens (J. pubescens) (Oleaceae) is an ornamental sub-shrub that contains various bioactive remedies that are exploited for various disorders. The objective of present study was focused on the phytochemical screening of Jasmine plant leaves for the presence of flavonoids, alkaloids, tannins, coumarins, terpenoids, proteins and amino acids in aqueous, methanolic and petroleum ether extracts. The antioxidant activity was determined spectrophotometry using DPPH and FRAP method and presence of total phenol and flavonoids were also determined. The agar well diffusion method was employed for antimicrobial activity against Bacillus subtilis, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. The results of study indicated that the methanolic extracts of Jasmine leaves showed significant antimicrobial activity against S. aureus (25.7±0.84mm), minimum inhibitory concentration (12.5mg/ml) as compared to other extracts and Ofloxacin as a standard. The methanolic, petroleum ether and aqueous leaf extracts of J. pubescens are utilized for production of newer bioactive phytometabolites having potent antimicrobial and antioxidant activity for curing various ailments.
Cite this article:
Jitendra Gupta. Preliminary Phytochemical Investigation, Antioxidant and Antimicrobial Activity of Jasminum pubescence Leaves Extracts. Research J. Pharm. and Tech. 2020; 13(12):6073-6076. doi: 10.5958/0974-360X.2020.01058.6
Cite(Electronic):
Jitendra Gupta. Preliminary Phytochemical Investigation, Antioxidant and Antimicrobial Activity of Jasminum pubescence Leaves Extracts. Research J. Pharm. and Tech. 2020; 13(12):6073-6076. doi: 10.5958/0974-360X.2020.01058.6 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2020-13-12-73
REFERENCES:
1. Colvard MD, Cordell GA. Rationalizing the Study of Plants for the Treatment of Oral Pain. Current Chemical Biology. 2008; 2(2): 140-152.
2. Sabharwal S, Sudan S, Ranjan V. Jasminum sambac Linn (Motia): A Review. International Journal of Pharmaceutical Research and Bio-Science. 2013; 2(5): 108-130.
3. Singh S, Jangde R, Daharwal SJ. Jasmine an Updated Review on Herbal Drug in Wound Healing. Research Journal of Pharmacy and Technology. 2019; 12(6): 3089-3097.
4. Thiruvengadam S, Nivedha S, Pujita V, Romauld SI. Detection of Antioxidant and Antimicrobial Activity of Leaf Extract of Jasminum azoricum. Research Journal of Pharmacy and Technology. 2018; 11(8): 3629-3632.
5. Temraz A, Cioni PL, Flamini G, Braca A. Chemical Composition of the Essential Oil from Jasminum pubescens Leaves and Flowers. Natural Product Communications. 2009; 4(12): 1729-1732.
6. Sabharwal S, Aggarwal S, Vats M, Sardana S. Jasminum sambac (Linn) Ait: Preliminary Phytochemical Screening and Wound Healing Investigation using Total Ethanol Stems Extract. International Journal of Pharmaceutical Sciences Review and Research. 2012; 17(1): 44-47.
7. http://mmmnaturals.blogspot.com/2016/07/types-of-jasmine_29.html (accessed on March 20, 2020)
8. Gupta R, Gupta J. Investigation of Antimicrobial Activity of Euphorbia Hirta Leaves. International Journal of Life science and Pharma Research. 2019; 9(3): 32-37.
9. Hemasudha TS, Yuvarani S, Rajakumari K. Combination of Azadirachta indica and Phyllanthus acidus - A complete Evaluation and Characterization Study. Research Journal of Pharmacy and Technology. 2019; 12(6): 3048-3052.
10. Britto JA, Gracelin DHS. Efficacy of Fruits of Jasminum grandiflorum Linn. Against Plant and Animal Pathogens. Asian Journal of Pharmaceutical and Clinical research. 2011; 4: 74-75.
11. Gupta R, Gupta MK, Bhandari A, Gupta J. Preliminary Pharmacognostical and Physicochemical Analysis: A Poly Herbomineral Formulation. International Journal of Drug Development and Research. 2014; 6(3): 85–92.
12. Thilagavathi T, Kathiravan G. Phytochemical Analysis and Antimicrobial Activity of Ethonolic Leaf Extract of Ficus racemosa Linn. Research Journal of Pharmacy and Technology. 2017; 10(2): 537-540.
13. Ruskin S, Ajina RS. Qualitative Phytochemical Screening and In-vitro Anthelmintic Activity of Adhatoda vasica (Acanthaceae). Research Journal of Pharmacy and Technology. 2017; 10(2): 414-420.
14. Nair DU, Saraswathy MP, Kishore N, Pully NR. Phytochemical and Antimicrobial Evaluation of Luffa cylindrica Linn. Leaf and Flower Extracts – An In-Vitro Study. Research Journal of Pharmacy and Technology. 2010; 3(2): 438-441.
15. Rahate KP, Padma R, Parvathy NG, Rengith V. Quantitative Estimation of Tannins, Phenols and Antioxidant Activity of Methanolic Extract of Imperata cylindrica. International Journal of Research in Pharmaceutical Sciences. 2013; 4(1): 73-77.
16. Gupta R, Gupta MK, Bhandari A, Gupta J. Preparation and Standardization of Polyherbomineral Formulation. International Journal of Drug Development and Research. 2014; 6(2): 211–9.
17. Prabha SB, Rao M, Kumar MRR. Evaluation of in vitro Antioxidant, Antibacterial and Anticancer Activities of Leaf Extracts of Cleome rutidosperma. Research Journal of Pharmacy and Technology. 2017; 10(8): 2492-2496.
18. Umavandhana R, Jayanthi S. Analysis of Phytochemical compounds and DPPH Radical Scavenging Activity of Dictyota dichotoma and Halimeda macroloba. Research Journal of Pharmacy and Technology. 2018; 11(8): 3440-3444.
19. Prakkash MAJ, Ragunathan R, Johney J. Evaluation of Bioactive Compounds from Jasminum polyanthum and its Medicinal Properties. Journal of Drug Delivery and Therapeutics. 2019; 9(2): 303-310.
20. Tiwari P, Patel RK. Estimation of Total Phenolics and Flavonoids and Antioxidant Potential of Ashwagandharishta Prepared by Traditional and Modern Methods. Asian Journal of Pharmaceutical Analysis. 2013; 3(4): 147-152.
21. Amini MH, Kalsi V, Kaur B, Khatik GL, Lobo R, Singh G, Agarhari UC, Yele S, Suttee A. Phytochemical Screening and Antioxidant Activity of Heracleum afghanicum Kitamura Leaves. Research Journal of Pharmacy and Technology. 2017; 10(10): 3498-3502.
22. Tiwari P. Phenolics and Flavonoids and Antioxidant Potential of Balarishta Prepared by Traditional and Modern Methods. Asian Journal of Pharmaceutical Analysis. 2014; 4(1): 05-10
23. Gupta J, Gupta R, Ahuja A. Formulation, Characterization and Antimicrobial Activity of Polyherbal Hand Wash. International Journal of Pharmaceutical Sciences and Research. 2020; 3: 45-47.
24. Kumar S, Navneet, Gautam SS. Screening of Antimicrobial Properties of Jasminum sambac Linn. Leaf Extracts Against Dental Pathogens. Research Journal of Phytochemistry. 2015; 9(4): 195-200.
25. Hemalatha M, Arirudran B, Thenmozhi A, Rao USM. Antimicrobial Effect of Separate Extract of Acetone, Ethyl Acetate, Methanol and Aqueous from Leaf of Milkweed (Calotropis gigantea L.). Asian Journal of Pharmaceutical Research. 2011; 1(4): 102-107
26. Shivsharan U, Ravva S. Antimicrobial Activity of Pomegranate Juice. Research Journal of Pharmacy and Technology. 2018; 10(11): 1-3.
27. Saraswathi K, Sivaraj C, Jenifer A, Dhivya M, Arumugam P. Antioxidant, Antibacterial Activities, GCMS and FTIR Analysis of Ethanol Bark Extract of Capparis sepiaria L. Research Journal of Pharmacy and Technology. 2020; 13(5): 2144-2150.