Author(s):
Nagesh Kishan Panchal, Jerine Peter S, Pratiksha Chhetri, Chandrayee Sil, Alumar Farook A Evan Prince Sabina
Email(s):
eps674@gmail.com
DOI:
10.5958/0974-360X.2020.01075.6
Address:
Nagesh Kishan Panchal, Jerine Peter S, Pratiksha Chhetri, Chandrayee Sil, Alumar Farook A Evan Prince Sabina*
Department of Biomedical Sciences, School of Bio Sciences and Technology, VIT, Vellore.
*Corresponding Author
Published In:
Volume - 13,
Issue - 12,
Year - 2020
ABSTRACT:
Diabetes is a condition that takes place when the sugar in the blood rises to high level. The food we intake gives us the energy that is provided by the blood glucose. The pancreas secreted hormone known as Insulin which aids glucose use “from” food for entering into the cells that is used for energy. Homology modelling is the technique to construct an atom based “resolution model” of the specific “target” protein from its respective amino acid sequences. It predicts the three-dimensional structure of a given protein sequence i.e. target protein. Around 1960’s Homology modelling studies were performed using models of bonds and atoms made up of plastic and wire. In this study, we conduct a literature survey to find out the genes responsible for diabetes toxicity followed by their protein modelling by utilizing their respective FASTA sequence from Uni Prot database. Then running the respective sequence of those genes in i-TASSER. The quality of the sequence will be verified through Rampage online software. This study aims for identifying and comparing the non-mutated genes and modelling them accordingly.
Cite this article:
Nagesh Kishan Panchal, Jerine Peter S, Pratiksha Chhetri, Chandrayee Sil, Alumar Farook A Evan Prince Sabina. In-silico docking analysis of the active compounds of Murraya koenigii seeds with the modelled protein of unstructured Diabetes genes. Research J. Pharm. and Tech. 2020; 13(12):6163-6169. doi: 10.5958/0974-360X.2020.01075.6
Cite(Electronic):
Nagesh Kishan Panchal, Jerine Peter S, Pratiksha Chhetri, Chandrayee Sil, Alumar Farook A Evan Prince Sabina. In-silico docking analysis of the active compounds of Murraya koenigii seeds with the modelled protein of unstructured Diabetes genes. Research J. Pharm. and Tech. 2020; 13(12):6163-6169. doi: 10.5958/0974-360X.2020.01075.6 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2020-13-12-90
REFERENCES:
1. Gould GW, Thomas HM, Jess TJ, Bell GI. Expression of human glucose transporters in Xenopus oocytes: kinetic characterization and substrate specificities of the erythrocyte, liver, and brain isoforms. Biochemistry. 1991;30(21):5139-5145.
2. Mathers CD, Loncar D. Projections of Global Mortality and Burden of Disease from 2002 to 2030. PLOS Medicine. 2006;3(11):e442.
3. Chothia C, Lesk AM. The relation between the divergence of sequence and structure in proteins. The EMBO Journal. 1986;5(4):823-826.
4. Martí-Renom MA, Stuart AC, Fiser A, Sánchez R, Melo F, Šali A. Comparative Protein Structure Modeling of Genes and Genomes. Annual Review of Biophysics and Biomolecular Structure. 2000;29(1):291-325.
5. McCulloch LJ, van de Bunt M, Braun M, Frayn KN, Clark A, Gloyn AL. GLUT2 (SLC2A2) is not the principal glucose transporter in human pancreatic beta cells: implications for understanding genetic association signals at this locus. Molecular Genetics and Metabolism. 2011;104(4):648-653.
6. Guillam MT, Hümmler E, Schaerer E, et al. Early diabetes and abnormal postnatal pancreatic islet development in mice lacking Glut-2. Nature Genetics. 1997;17(3):327-330.
7. Uldry M, Ibberson M, Hosokawa M, Thorens B. GLUT2 is a high affinity glucosamine transporter. FEBS Letters. 2002;524(1):199-203.
8. (7) (PDF) Loss of Sugar Detection by GLUT2 Affects Glucose Homeostasis in Mice. ResearchGate. https://www.researchgate.net/publication/5774477_Loss_of_Sugar_Detection_by_GLUT2_Affects_Glucose_Homeostasis_in_Mice. Accessed November 29, 2019.
9. Bedford FK, Ashworth A, Enver T, Wiedemann LM. HEX: a novel homeobox gene expressed during haematopoiesis and conserved between mouse and human. Nucleic Acids Research. 1993;21(5):1245-1249.
10. Hallaq H, Pinter E, Enciso J, et al. A null mutation of Hhex results in abnormal cardiac development, defective vasculogenesis and elevated Vegfa levels. Development (Cambridge, England). 2004;131(20):5197-5209.
11. Savan R, Kono T, Igawa D, Sakai M. A novel tumor necrosis factor (TNF) gene present in tandem with theTNF-alpha gene on the same chromosome in teleosts. Immunogenetics. 2005;57(1-2):140-150.
12. Spies T, Morton CC, Nedospasov SA, Fiers W, Pious D, Strominger JL. Genes for the tumor necrosis factors alpha and beta are linked to the human major histocompatibility complex. Proceedings of the National Academy of Sciences of the United States of America. 1986;83(22):8699-8702.
13. Pennica D, Nedwin GE, Hayflick JS, et al. Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin. Nature. 1984;312(5996):724-729.
14. Ali O. Genetics of type 2 diabetes. World Journal of Diabetes. 2013;4(4):114-123.
15. Reue K, Donkor J. Genetic factors in type 2 diabetes: all in the (lipin) family. Diabetes. 2007;56(12):2842-2843.
16. Civitarese AE, Jenkinson CP, Richardson D, et al. Adiponectin receptors gene expression and insulin sensitivity in non-diabetic Mexican Americans with or without a family history of Type 2 diabetes. Diabetologia. 2004;47(5):816-820.
17. McCarthy MI. Growing evidence for diabetes susceptibility genes from genome scan data. Current Diabetes Reports. 2003;3(2):159-167.
18. McCarthy MI. Genomics, Type 2 Diabetes, and Obesity. New England Journal of Medicine. 2010;363(24):2339-2350.