Author(s): Akash Aravindhan M, Gayathri T, Sakthiselvan P


DOI: 10.5958/0974-360X.2020.00261.9   

Address: Akash Aravindhan M1, Gayathri T1, Sakthiselvan P1
1Department of Bio-Engineering, School of Engineering, Vels Institute of Science Technology and Advanced Studies (VISTAS), Pallavaram, Chennai – 600117, Tamil Nadu, India.
*Corresponding Author

Published In:   Volume - 13,      Issue - 3,     Year - 2020

Dyes are harmful for living organisms. Nerium oleander was used to degrade the synthetic Congo red dye. Optimization process was carried out for better degradation of synthetic dye using Placket-Burman design. Among the six parameters (Temperature, Incubation time, pH, Agitation speed, Biomass concentration, Dye concentration), pH (5), Temperature (35 ?) and Incubation time (60 min) were found to increase the dye degradation (92.33 %). The global optimum levels of dye degradation were analyzed using Plackett-Burman design. The effects of pH, Temperature and Incubation time on degradation were further optimized by statistical analysis using Response Surface Methodology (RSM). RSM was used to optimize dye degradation by implementing the Central Composite Design (CCD). The optimal conditions for higher degradation were pH: 5, Temperature: 35?, and Incubation time: 90 min. Under these conditions, the model predicted a dye degradation of 98.23 %. Verification of the optimization showed that dye degradation of 97.18 % was observed under the optimal conditions in the shake flasks experiment.

Cite this article:
Akash Aravindhan M, Gayathri T, Sakthiselvan P. Optimization Studies on Degradation of Congo Red Dye using Nerium oleander. Research J. Pharm. and Tech 2020; 13(3):1429-1434. doi: 10.5958/0974-360X.2020.00261.9

Akash Aravindhan M, Gayathri T, Sakthiselvan P. Optimization Studies on Degradation of Congo Red Dye using Nerium oleander. Research J. Pharm. and Tech 2020; 13(3):1429-1434. doi: 10.5958/0974-360X.2020.00261.9   Available on:


1.     Altaf A, Noor S, Sharif QM, Najeebullah M. Different techniques recently used for the treatment of textile dyeing effluents: A review. Journal of the Chemical Society of Pakistan. 2010;32(1):115-6.

2.     Muthukumaran P M. Alamelumangai. Mathumitha M.Decolorization and Degradation of Reactive Dyes by Aspergillus niger. Research J. Engineering and Tech. 2013;4(4): 235-238.

3.     Dontulwar J R. Borikar D K.  Biodegradation and Spectroscopic Study of Polymer Synthesized from Starch, Glycerol, Sorbitol, Maleic Anhydride as Precursor. Asian J. Research Chem.2011; 4(7):1084-1086.

4.     Chung KT, Stevens SE, Cerniglia CE. The reduction of azo dyes by the intestinal microflora. Critical reviews in microbiology. 1992 Jan 1;18(3):175-90.

5.     Athira G, Jacyntha T, Narendrakumar G, Preethi T V. Application of Response Surface Methodology (RSM) to optimize culture media for the production of rhamnolipids by Pseudomonas aeruginosa. Research J. Pharm. and Tech. 2016; 9(4): 335-339.

6.     Aravinth S S E and Ezhil Priya M. Degradation of Dyeing Effluent Using Fenton’s Oxidation. Research J. Engineering and Tech. 2013; 4(4): 217-220.

7.     Devika R, Shalini C, Subbaiya R. Biosynthesis of Copper Nanoparticle using Nerium oleander. Research J. Pharm. and Tech. 2019; 12(2):621-624.

8.     Praveen Kumar GN, Bhat Sumangala K. Decolorization of azo dye Red 3BN by bacteria. International Research Journal of Biological Sciences. 2012;1(5):46-52.

9.     Sharma DC, Satyanarayana T. A marked enhancement in the production of a highly alkaline and thermostable pectinase by Bacillus pumilus dcsr1 in submerged fermentation by using statistical methods. Bioresource Technology. 2006 Mar 1;97(5):727-33.

10. Keharia H, Patel H, Madamwar D. Decolorization screening of synthetic dyes by anaerobic methanogenic sludge using a batch decolorization assay. World Journal of Microbiology and Biotechnology. 2004 Jun 1;20(4):365-70.

11. Zhang Q, Chuang KT. Adsorption of organic pollutants from effluents of a Kraft pulp mill on activated carbon and polymer resin. Advances in Environmental Research. 2001 Aug 1;5(3):251-8. Mohini U,

12. Snehal A, Prajkta D, Dhole S N. Pharmacognostic Study of Leaf and Bark of Nerium oleander and Eugenia jambolana. Research Journal of Pharma and Phytochem. 2012; 4(5): 277-279.

13. Ghurghure S M, Dhange A A, Kamalapure N R, Kate S N, Katkar A R, Katta A V, Kattimani C U, Kattimani S G.Formulation and Evaluation of Herbal Hand Wash Gel using Nerium oleander. Research J. Topical and Cosmetic Sci.2019;10(1):01-06.

14. Gaikwad B G. Biodegradation of Black Liquor. Research J.  Science and Tech.2015; 7(1):47-51.

15. Amjed M O, Hsan K N, Abbas J L, Ahmed S, Luma A, Hayder J, Kadhim F.Wastewaters over a neat and silver Doped Zinc Oxide Nanoparticles. A Kinetics Study. Research J. Pharm. and Tech. 2019; 12(6): 2669-2676.

16. Pallavi1 P, King P, Prasanna K. Removal of Methylene Blue from an Aqueous Solution using Plumbago zeylanica Leaves-Optimisation using Response Surface Methodology (RSM). Research J. Pharm. and Tech 2018; 11(5):2006-2016.

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