Vadivelan Ramachandran, Gonala Vijay Kumar, Sudeep Sugumar, Vikash Sundaram, Haja Nazeer Ahamed
Vadivelan Ramachandran1, Gonala Vijay Kumar1, Sudeep Sugumar1, Vikash Sundaram1, Haja Nazeer Ahamed2
1Department of Pharmacology, JSS College of Pharmacy, (JSS Academy of Higher Education & Research), Ootacamund, Nilgiris, Tamilnadu - 643001.
2Crescent School of Pharmacy, B.S. Abdur Rahman Crescent Institute of Science and Technology, Vandalur, Chennai, Tamilnadu - 600048.
Volume - 13,
Issue - 4,
Year - 2020
The study was designed to evaluate the hydro-alcoholic extract of Tridax Procumbens Linn (THAE) on lipids, lipoproteins and antioxidants activity in isoproterenol (ISO) induced myocardial infarcted rats. Myocardial infarction was induced by ISO (85mg/kg, s.c.) for two consecutive days at an interval of 24 hours. Rats were pretreated with THAE (100 and 200mg/kg, oral) for a period of 20 days and (ISO) was injected on 21 and 22 days at 24 hr intervals and after 24hr blood was collected through retro-orbital plexus for the estimation biochemical and antioxidant parameters. ISO caused a significant increase in the concentration of total cholesterol, triglycerides, low-density lipoprotein cholesterol (LDL-C), very low-density lipoprotein cholesterol (VLDL-C) and lipid peroxidation (LPO) whereas a significant decrease in high-density lipoprotein cholesterol (HDL-C). ISO administration also significantly decreased paraoxonase (PON) enzyme. Oral pretreatment of THAE at doses 100 and 200mg/kg for 20 days challenged with a concurrent injection of ISO (85mg/kg bw) on 21 and 22 days significantly attenuated these alterations and restored the levels of lipids, lipoproteins. In addition, THAE significantly alleviated the serum antioxidants enzymes PON, LPO and Catalase (CAT). The present findings shows that pretreatment of THAE in ISO injected rats significantly attenuates myocardial infarction.
Cite this article:
Vadivelan Ramachandran, Gonala Vijay Kumar, Sudeep Sugumar, Vikash Sundaram, Haja Nazeer Ahamed. Cardioprotective effect of Tridax procumbens Linn in Isoproterenol Induced Myocardial Infarction in rats. Research J. Pharm. and Tech. 2020; 13(4):1921-1925. doi: 10.5958/0974-360X.2020.00346.7
Vadivelan Ramachandran, Gonala Vijay Kumar, Sudeep Sugumar, Vikash Sundaram, Haja Nazeer Ahamed. Cardioprotective effect of Tridax procumbens Linn in Isoproterenol Induced Myocardial Infarction in rats. Research J. Pharm. and Tech. 2020; 13(4):1921-1925. doi: 10.5958/0974-360X.2020.00346.7 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2020-13-4-56
1. Upaganlawar A, Gandhi H, Balaraman R. Isoproterenol induced myocardial infarction: Protective role of natural products. J Pharmacol Toxicol. 2011; 6(1): 1-17.
2. Whellan, DJ. Heart failure disease management: implementation and outcomes. Cardiol Rev. 2005; 13(5): 231-9.
3. Ravichandran V, Hanumantharayappa B, Madhava Reddy Papasani V. Evaluation of cardioprotective activity of galangin against doxorubicin induced cardiomyopathy. Int. J. Pharm Sci. 2014; 6(9):86-90.
4. Abdel R. Cardioprotective efficacy of taurine on lipid-metabolism of isoproterenol-induced myocardial infarction. Int. J. Pharm Sci. 2016; 8(12):537-41.
5. Singal PK, Kapur N, Dhillon KS, Beamish RE, Dhalla NS. Role of free radicals in catecholamine-induced cardiomyopathy. Can J Physiol Pharmacol.1982; 160(11):1390-7.
6. Naghavi M, Libby P, Falk E, Casscells SW, Litovsky S, Rumberger J et al. From vulnerable plaque to vulnerable patient: a call for new definitions and risk assessment strategies: Part I. Circulation. 2003; 108(14):1664-72.
7. Durrington PN, Mackness B, Mackness MI: Paraoxonase and atherosclerosis. Arterioscler Thromb Vasc Biol. 2001; 21(4): 473-480.
8. Rozenberg O, Rosenblat M, Coleman R, Shih DM, Aviram M. Paraoxonase (PON1) deﬁciency is associated with increased macrophage oxidative stress: studies in PON1-knockout mice. Free Radic Biol Med. 2003; 34(6):774–784.
9. Kuo CL, La Du BN. Comparison of purified human and rabbit serum paraoxonases: Drug Metab Dispos. 1995; 23(9):935-44.
10. Saraf S, Pathak A, Dixit VK. Hair growth promoting activity of Tridax procumbens. Fitoterapia. 1991; 62:495-498.
11. Taddei A, Rosas-Romero AJ. Bioactivity studies of extracts from Tridax procumbens. Phytomedicine. 2000; 7 (3):235-238.
12. Udupa SL, Udupa AL, Kulkarni DR. Influence of Tridax procumbens on lysyl oxidase activity and wound healing. Planta Med. 1991; 57(4):325-27.
13. Tiwari U, Rastogi B, Singh P, Saraef DK, Vays SP. Immunomodulatory effects of aqueous extract of Tridax procumbens in experimental animals. J Ethnopharmacol. 2004; 92 (1):113-119.
14. Salahdeen HM, Yemitan OK, Alada ARA. Effect of aqueous leaf extract of Tridax procumbens on blood pressure and heart rate in rats: African J Biomed Res. 2004; 7:27-29.
15. Ravikumar V, Shivashangari KS, Devaki T. Hepatoprotective activity of Tridax procumbens against d-galactosamine/lipopolysaccharide-induced hepatitis in rats. J Ethnopharmacol. 2005; 101(1-3):55-60.
16. Yadawa RN, Saurabh K. A new flavone glycoside. 5, 7, 4-Trihydraxy- 6,3-dimethasey Falavone 5-0 alpha-L-rhamnopyramoside from the leaves of Tridax procumbens Linn. J Asian Nat Prod Res. 1998; 1(2):147-52.
17. Ali M, Rawinder E, Ramchandran R. A new flavonoid from the aerial parts of Tridax procumbens: Fitoterapia. 2001; 72 (3):313-315.
18. Ali MS, Jahangir M. A bis-bithiophene from Tridax procumbens L. (Asteraceae). Nat Prod Lett. 2002; 16(4):217-221.
19. Williamson EM, Okpako DT, Evans FJ. Pharmacological methods in phytotherapy research. John Wileyand Sons; 1998.
20. Md. Ibrahim Khalil, Istiyak Ahmmed, Romana Ahmed, E. M. Tanvir, Rizwana Afroz, Sudip Paul et al. Amelioration of isoproterenol-induced oxidative damage in rat myocardium by Withania somnifera leaf extract. BioMed Research International. 2015; http://dx.doi.org/10.1155/2015/624159.
21. Allain CC, Poon LS, Chan CS, Richmond W, Fu PC. Enzymatic determination of total serum cholesterol. Clin Chem. 1974; 20(4): 470-5.
22. Richmond W. Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic activity assay of total cholesterol in serum. Clin Chem, 1973; 19(12):1350-6.
23. Okhawa H, Oohishi N, Yagi N: Assay for lipid peroxides in animal tissues by the thiobarbituric acid reaction. Anal Biochem. 1979; 95(2):351-358.
24. Gan A, Smolen HW, Eckerson BN, La Du. Puriﬁcation of human serum paraoxonase/ arylesterase. Evidence for one esterase catalyzing both activities. Am Soc Pharmacol Exp Ther. 1991; 19(1): 100-6.
25. Gutteridge, J.M.C., Free radicals damage to lipids, amino acids, carbohydrates and nucleic acids, determined by TBA reactivity. Int. J. Biochem. 1982; 14 (7): 649-654.
26. Jagadeesh, G.S., Nagoor Meeran, M.F., Selvaraj, P. Protective effects of 7-hydroxycoumarin on dyslipidemia and cardiac hypertrophy in isoproterenol-induced myocardial infarction in rats. J Biochem Mol Toxicol. 2016; 30(3):120-7
27. Prince SMP, Rajkumar S, Dhanasekar K. Protective effects of vanillic acid on electrocardiogram, lipid peroxidation, antioxidants, proinflammatory markers and histopathology in isoproterenol induced cardiotoxic rats. Eur J Pharmacol. 2011; 668 (1-2):233-240.
28. Shaik AH, Shaik NR, Mohammed AK, Omar SY, Mohammad A, Mohaya TA, et al. Terminalia pallida fruit ethanolic extract ameliorates lipids, lipoproteins, lipid metabolism marker enzymes and paraoxonase in isoproterenol-induced myocardial infarcted rats. Saudi J Biol Sci. 2018; 25(3):431-6.