Author(s):
Vaishnav Lal C. L, R. S Chandan, Maruthi. R, Anand Kumar Tengli
Email(s):
rschandan@jssuni.edu.in
DOI:
10.5958/0974-360X.2020.00659.9 
Address:
Vaishnav Lal C. L, R. S Chandan, Maruthi. R, Anand Kumar Tengli
Department of Pharmaceutical Chemistry, JSS College of Pharmacy, Mysuru, JSS Academy of Higher Education and Research, Sri Shivarathreeshwara Nagara, Mysuru- Karnataka, India 570015.
*Corresponding Author
Published In:
Volume - 13,
Issue - 8,
Year - 2020
ABSTRACT:
Organophosphates are a group of pesticides that were developed in the 1940s in Germany andsoon became a significant defense against agricultural pests. The organophosphate Dichlorvos is a commonly used group of pesticide is a broad-spectrum organophosphate compound having insecticidal activity. Dichlorvos is a cholinesterase inhibitor exhibiting stomach, contact and systemic mode of action. The development of bioanalytical method is the process of developing a procedure for the detection and quantification of a compound of interest in a biological fluid. A simple, reliable, fast, accurate and economical RP-UFLC method have been developed and validated for quantitative determination of Dichlorvos. The RP-UFLC procedure is developed by using ACN and Millipore water 50:50 v/v as mobile phase, Flow rate is maintained at 1.5mL/minute. Detection of Dichlorvos was performed by using PDA detector at 200nm. By this RP-UFLC procedure retention time (RT) of Dichlorvos was identified at 2.9 min.
Cite this article:
Vaishnav Lal C. L, R. S Chandan, Maruthi. R, Anand Kumar Tengli. Bio-Analytical Method Development and Validation of Dichlorvos Pesticide by RP-UFLC Method. Research J. Pharm. and Tech. 2020; 13(8):3725-3728. doi: 10.5958/0974-360X.2020.00659.9
Cite(Electronic):
Vaishnav Lal C. L, R. S Chandan, Maruthi. R, Anand Kumar Tengli. Bio-Analytical Method Development and Validation of Dichlorvos Pesticide by RP-UFLC Method. Research J. Pharm. and Tech. 2020; 13(8):3725-3728. doi: 10.5958/0974-360X.2020.00659.9 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2020-13-8-32
REFERENCE:
1. Costa LG. Current issues in organophosphate toxicology. Clin Chim Acta. 2006;366 (1-2):1-13.
2. Kwong TC. Organophosphate pesticides: Biochemistry and clinical toxicology. Ther Drug Monit. 2002;24(1):144–9.
3. Rusyniak DE, Nañagas KA. Organophosphate poisoning. Vol. 24, Seminars in Neurology. 2004. 197–204 .
4. Sukirtha TH, Usharani M V, Jones OAH, Voulvoulis N, Lester JN, Kumar S, et al. Original Research Article Gas Chromatography-Mass Spectrometry Determination of Organophosphate Pesticide Residues in water of the irrigation canals in the North Zone ,Tamil Nadu , India. Sci Total Environ [Internet]. 2013;2(January):321–9. Available from: http://dx.doi.org/10.1016/j.scitotenv.2014.12.041
5. Jones mw, sommerville c, wootten r. Reduced sensitivity of the salmon louse, Lepeophtheirus salmonis, to the organophosphate dichlorvos. J Fish Dis. 1992;15(2):197–202.
6. Blair D, Dix KM, Hunt PF, Thorpe E, Stevenson DE, Walker AIT. Dichlorvos - a 2-year inhalation carcinogenesis study in rats. Arch Toxicol. 1976;35(4):281–94.
7. Sharma VK, Jadhav RK, Rao GJ, Saraf AK, Chandra H. High performance liquid chromatographic method for the analysis of organophosphorus and carbamate pesticides. Forensic Sci Int. 1990;48(1):21–5.
8. Pavan Kumar C, Gurupadayya BM. Analytical method development and validation of dimethoate pesticide using HPLC method. Anal. Biochem, 2013; 2:2161-1009.
9. Parrilla P, Vidal JM, Galera MM, Frenich AG. Simple and rapid screening procedure for pesticides in water using SPE and HPLC/DAD detection. Fresenius' journal of analytical chemistry. 1994 Oct 1;350(10-11):633-7.
10. Inoue S, Saito T, Mase H, Suzuki Y, Takazawa K, Yamamoto I, et al. Rapid simultaneous determination for organophosphorus pesticides in human serum by LC-MS. J Pharm Biomed Anal. 2007;44(1):258–64.