Muthu Mohamed J, Fazil Ahmad, Narra Kishore, Abeer M. Al-Subaie
Muthu Mohamed J*1, Fazil Ahmad2, Narra Kishore1, Abeer M. Al-Subaie3
1Department of Pharmaceutical Technology, BIT Campus, Anna University, Tiruchirappalli 620024, Tamil Nadu, India.
2Department of Anesthesia Technology, College of Applied Medical Sciences in Jubail, Imam Abdulrahman Bin Faisal University, P.O. Box 4030, Jubail, Saudi Arabia.
3Department of Clinical laboratory Sciences, College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam, Saudi Arabia.
Volume - 14,
Issue - 1,
Year - 2021
This study investigates the solid dispersion (SD) of curcumin (CMN) enhance the solubility, which can be employed for the treatment of colorectal cancer (CRC) greater to pure CMN. Solid dispersion (SD) prepared by a hot melt method using CMN with several carriers of poloxamers (P-407 and P-188), gelucire 50/13 (GLR) and mannitol (MNT). Prior, phase solubility studies were performed with drug and carriers. The SD characterized by in vitro drug release and novel dyeing test. Additionally, the cytotoxicity and apoptosis studies resolved to utilize the colorectal adenocarcinoma cell lines. The result showed that CMN-P-407 complex produced significant properties towards solubility (318±14.46 fold) and dissolution (91±0.43% at 30 min). The IC50 value for complex found to be 74 and 52µM/mL, while that for pure CMN ranged from 146 and 116µM/mL on the SW480 and Caco-2 cells respectively. Apoptosis study revealed that the cells are undergoing cell death by apoptosis and the small number of necrosis. The profound ef?ciency of soluble 1:1 stoichiometry curcumin binary complex (CMN-P-407 SD) indicated its potential application for CRC treatment by showing a higher capability of inhibiting cell growth compared to that of pure CMN.
Cite this article:
Muthu Mohamed J, Fazil Ahmad, Narra Kishore, Abeer M. Al-Subaie. Soluble 1:1 Stoichiometry curcumin binary complex for potential apoptosis in human colorectal adenocarcinoma cells (SW480 and Caco-2 cells). Research J. Pharm. and Tech. 2021; 14(1):129-135. doi: 10.5958/0974-360X.2021.00023.8
Muthu Mohamed J, Fazil Ahmad, Narra Kishore, Abeer M. Al-Subaie. Soluble 1:1 Stoichiometry curcumin binary complex for potential apoptosis in human colorectal adenocarcinoma cells (SW480 and Caco-2 cells). Research J. Pharm. and Tech. 2021; 14(1):129-135. doi: 10.5958/0974-360X.2021.00023.8 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-1-23
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