Author(s):
Muthu Mohamed J, Fazil Ahmad, Narra Kishore, Abeer M. Al-Subaie
Email(s):
jmuthumohamed@gmail.com
DOI:
10.5958/0974-360X.2021.00023.8 
Address:
Muthu Mohamed J*1, Fazil Ahmad2, Narra Kishore1, Abeer M. Al-Subaie3
1Department of Pharmaceutical Technology, BIT Campus, Anna University, Tiruchirappalli 620024, Tamil Nadu, India.
2Department of Anesthesia Technology, College of Applied Medical Sciences in Jubail, Imam Abdulrahman Bin Faisal University, P.O. Box 4030, Jubail, Saudi Arabia.
3Department of Clinical laboratory Sciences, College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam, Saudi Arabia.
*Corresponding Author
Published In:
Volume - 14,
Issue - 1,
Year - 2021
ABSTRACT:
This study investigates the solid dispersion (SD) of curcumin (CMN) enhance the solubility, which can be employed for the treatment of colorectal cancer (CRC) greater to pure CMN. Solid dispersion (SD) prepared by a hot melt method using CMN with several carriers of poloxamers (P-407 and P-188), gelucire 50/13 (GLR) and mannitol (MNT). Prior, phase solubility studies were performed with drug and carriers. The SD characterized by in vitro drug release and novel dyeing test. Additionally, the cytotoxicity and apoptosis studies resolved to utilize the colorectal adenocarcinoma cell lines. The result showed that CMN-P-407 complex produced significant properties towards solubility (318±14.46 fold) and dissolution (91±0.43% at 30 min). The IC50 value for complex found to be 74 and 52µM/mL, while that for pure CMN ranged from 146 and 116µM/mL on the SW480 and Caco-2 cells respectively. Apoptosis study revealed that the cells are undergoing cell death by apoptosis and the small number of necrosis. The profound ef?ciency of soluble 1:1 stoichiometry curcumin binary complex (CMN-P-407 SD) indicated its potential application for CRC treatment by showing a higher capability of inhibiting cell growth compared to that of pure CMN.
Cite this article:
Muthu Mohamed J, Fazil Ahmad, Narra Kishore, Abeer M. Al-Subaie. Soluble 1:1 Stoichiometry curcumin binary complex for potential apoptosis in human colorectal adenocarcinoma cells (SW480 and Caco-2 cells). Research J. Pharm. and Tech. 2021; 14(1):129-135. doi: 10.5958/0974-360X.2021.00023.8
Cite(Electronic):
Muthu Mohamed J, Fazil Ahmad, Narra Kishore, Abeer M. Al-Subaie. Soluble 1:1 Stoichiometry curcumin binary complex for potential apoptosis in human colorectal adenocarcinoma cells (SW480 and Caco-2 cells). Research J. Pharm. and Tech. 2021; 14(1):129-135. doi: 10.5958/0974-360X.2021.00023.8 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-1-23
REFERENCE:
1. Nathan CH, Alexander TR. Colorectal Cancer: Imaging Conundrums. Surgical Oncology Clinics of North America 2018; 27:289-302.
2. Alberto F, Valeria Z, Antonio M, et al. Mediterranean diet and colorectal cancer: A systematic review. Nutrition 2017; 43-44:83-88.
3. Bansal SS, Goel M, Aqil F, et al. Advanced drug delivery systems of curcumin for cancer chemoprevention. Cancer Prev Res (Phila) 2011; 4:1158-1171.
4. Higuchi T, Connors KA. Phase-solubility techniques. Adv Anal Chem Instrum 1965; 4:117-212.
5. Fernandes CM, Vieira MT, Veiga FJB. Physicochemical characterization and in vitro dissolution behaviour of nicardipine-cyclodextrins inclusion compounds. Eur J Pharm. Sci 2002; 15:79-88.
6. Chutimaworapan S, Oguchi G, Ritthidej C, et al. Effect of water-soluble carriers on dissolution characteristics of nifedipine solid dispersions. Drug Dev Ind Pharm 2000; 26:1141-1150.
7. Almeida A, Possemiers S, Boone M, et al. Ethylene vinyl acetate as a matrix for oral sustained release dosage forms produced via hot-melt extrusion. Eur J Pharm Biopharm 2011; 77: 297-305.
8. Lanxiang L, Juan X, Hua Z, et al. Inclusion complexes of laccaic acid A with ß-cyclodextrin or its derivatives: Phase solubility, solubilization, inclusion mode, and characterization. Dyes and Pigments 2017; 139: 737-746.
9. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983; 65:55-63.
10. Karthik S, Sankar R, Varunkumar K, et al. Romidepsin induces cell cycle arrest, apoptosis, histone hyperacetylation and reduces matrix metalloproteinases 2 and 9 expressions in bortezomib sensitized non-small cell lung cancer cells. Biomed Pharmacother 2014; 68: 327-334.
11. Kasibhatla GPAH, Finucane D, Brunner T, et al. Protocol: Staining of suspension cells with Hoechst 33258 to detect apoptosis. Cell: A Laboratory Manual Culture and Biochemical Analysis of Cells 1998; CSHL Press: USA. 1:15.5.
12. Le HT, Jeon HM, Choon Woo Lim CW, et al. Synthesis, Cytotoxicity, and phase-Solubility Study of Cyclodextrin Click Clusters. J Pharm Sci 2014; 103:3183-3189.
13. Eunmi B, Mijung P, Seonghee J, et al. Poloxamer-Based Thermoreversible Gel for Topical Delivery of Emodin: Influence of P407 and P188 on Solubility of Emodin and its Application in Cellular Activity Screening. Molecules 2017; 22: 1-10.
14. Ali W, Williams AC, Rawlinson CF. Stoichiometrically governed molecular interactions in drug: Poloxamer solid dispersions. Int J Pharm 2010; 391:162-168.
15. Nayak AK, Pal D. Formulation, optimization, and evaluation of jackfruit seed starch alginate mucoadhesive beads of metformin HCl. Int J Biomac 2013; 59:264-272.
16. Suresh B, Subash J, Basanth BE, et al. Enhancement of Solubility and Dissolution Rate of Loratadine with Gelucire 50/13. J Pharm Innov 2014; 9:141-149.
17. Iacovino R, Caso JV, Rapuano F, et al. Physicochemical Characterization and Cytotoxic Activity Evaluation of Hydroxymethyl ferrocene: β-Cyclodextrin Inclusion Complex. Molecules 2012; 17:6056-6070.
18. Yadav VR, Suresh S, Devi K, et al. Effect of Cyclodextrin Complexation of Curcumin on its Solubility and Antiangiogenic and Anti-inflammatory Activity in Rat Colitis Model. AAPS Pharm SciTech 2009; 10:752-762.
19. Le HT, Jeon HM, Lim CW, et al. Synthesis, Cytotoxicity, and phase-Solubility Study of Cyclodextrin Click Clusters. J Pharm Sci 2014; 103:3183-3189.
20. Cesareo J, Santosa J, Perez-Martíneza J, et al. Enhancement of albendazole dissolution properties using solid dispersions with Gelucire 50/13 and PEG 15000. J Drug Deliv Sci-Tech 2017; 42:261-272.
21. Valizadeh H, Nokhodchi A, Qarakhani N, et al. Physicochemical characterization of solid dispersions of indomethacin with PEG 6000, myrj 52, lactose, sorbitol, dextrin, and eudragit E100. Drug Dev Ind Pharm 2004; 30:303-317.
22. Ban E, Park M, Jeong S, et al. Poloxamer-Based Thermoreversible Gel for Topical Delivery of Emodin: Influence of P407 and P188 on Solubility of Emodin and Its Application in Cellular Activity Screening. Molecules. 2017; 22: 1-10.
23. Bujnakova M, Balaz M, Zduriencikova M, et al. Preparation, properties and anticancer effects of mixed As4S4/ZnS nanoparticles capped by Poloxamer 407. Mat Sci Eng: C 2017; 71:541-551.
24. Nayak AK, Pal D. Formulation, optimization, and evaluation of jackfruit seed starch alginate mucoadhesive beads of metformin HCl. Int J Biomac 2013; 59: 264–272.
25. Manju S, Sreenivasan K. Gold nanoparticles generated and stabilized by water-soluble curcumin–polymer conjugate: Blood compatibility evaluation and targeted drug delivery onto cancer cells. J Coll Inter Sci 2012; 368:144-151.
26. Dhivya R, Palaniandavar M, Jaividhya P, et al. In vitro antiproliferative and apoptosis-inducing properties of mononuclear copper(II) complex with the dppz ligand, in two genotypically different breast cancer cell lines. Biometals 2015; 28: 929-943.
27. Vignesh G, Senthilkumar R, Periasamy VS, et al. Protein binding and biological evaluation of polymer-anchored cobalt(III) complex containing a 2,2⁄ bipyridine ligand. RSC Adv 2014; 4: 57483-57492.