The main objective of this study was to evaluate in vitro antilithiatic activity of ethanolic extract of roots of Ipomoea digitata Linn. Inhibitory effect of extract on in vitro crystallization was analysed by nucleation assay. Cystone tablet a marketed drug used as a standard drug which is dispersed into 100ml buffer solution for compare of various concentration of ethanolic root extract of Ipomoea digitata Linn. The inhibitory activity of root extract on the nucleation of calcium oxalate crystals and the rate of aggregation in calcium oxalate crystals was determined by spectrophotometric assay. Investigation revealed that ethanolic extract of roots of Ipomoea digitata Linn is positive effect on inhibition for formatting precipitation of calcium and potassium in vitro.
Cite this article:
Kamta Prasad Namdeo, Sumanta Naskar, Neeli Rose Beck. In vitro Antilithiatic study of Ethanolic extract of roots of Ipomoea digitata Linn. Research J. Pharm. and Tech. 2021; 14(1):369-372. doi: 10.5958/0974-360X.2021.00067.6
Kamta Prasad Namdeo, Sumanta Naskar, Neeli Rose Beck. In vitro Antilithiatic study of Ethanolic extract of roots of Ipomoea digitata Linn. Research J. Pharm. and Tech. 2021; 14(1):369-372. doi: 10.5958/0974-360X.2021.00067.6 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-1-67
1. Soucie JM, Thun MJ, Coates RJ, McClellan W, Austin H. Demographic and geographic variability of kidney stones in the United States. Kidney Int. 1994; 46:893–899.
2. Hughes P. Caring for Australians with Renal Impairment (CARI). The CARI guidelines. Kidney stones epidemiology. Nephrology (Carlton), 2007; 12: 26–30.
3. Adriano R, Corrado V, Martino M, Epidemiology of nephrolithiasis, J. nephrol 2000; 13: 65-70.
4. Nabi G , Downey P, Keeley F, Watson G, Mc Clinnton S, Extracorporeal shock wave lithotripsy versus uretenoscopic management for ureteric clculi cohrane database system Rev, 2007;
5. Joseph KC, Bharat B, Parekh, Joshi MJ Inhibition of growth of urinary type calcium hydrogen phosphate dihydrate crystals by tartaric acid and Tamarind, Current Science, 2001; 88: 1232-1238.
6. Kirtikar KR, Basu BD, Indian medicinal plants, Oriental enterprises, Dehra Dun Uttrachal, 2nd edition, vol 7, 2001, 2369-2071.
7. Kokate CK, Practical Pharmacognosy, New Delhi, India Vallabh Prakashan 1991.
8. Evans WC, Trease and Evans Pharmacognosy, Edinburgh, UK Saunders/ Elsevier Limited 2009.
9. Phatak RS, Henhre AS, Invitro antiurolithiatic activity of Kalanchoe pinnata extract, International journal of Pharmacognosy and phytochemistry research 2015; 7(2) :275-279.
10. Patel MA, Patel PK, Seth AK, Inhibition of calcium oxalate crystallization by the fruit extracts of Piper nigrum L, Pharmacologyonline, 2011, 2: 1169-1177.
11. Mamillapalli V, Khantamneni PL, Mohammad Z, Mathangi A, Nandigam N, Namburi M, Katta V, Phytochemical and in vitro antiurolithiatic studies on the leaf extracts of Bauhinia variegata Linn. International Journal of Pharmaceutical Sciences and Research 2016; 7(10): 4074-84.
12. Khan SR, Reactive Oxygen Species as the Molecular Modulators of Calcium Oxalate Kidney Stone Formation: Evidence from Clinical and Experimental Investigations, J urol 2013;189 (3): 803-8011.
13. Erickson SB, Vrtiska TJ, Lieske JC, Effect of Cystone on urinary composition and stone formation over a one year period, Phytomedicine 2011; 18 : 863-867.
14. Patel PK, Patel MA, Vyas BA, Antiurolithiatic activity of saponin rich fraction from the fruits of Solanum xanthocarpum Schrad. and Wendl. (Solanaceae) against ethylene glycol induced urolithiasis in rats. J Ethnopharmacol, 2012; 144:16–70.
15. Saha S, Verma RJ. Inhibition of calcium oxalate crystallization in vitro by an extract of Bergenia ciliata. Arab Journal of Urology 2013; 11: 187-192.
16. Jyothilakshmi V, Thellamudhu G, Chinta R, Beneficial antioxidative effect of the homeopathic preparation of Berberis vulgaris in alleviating oxidative stress in experimental urolithiasis. Forsch Komplementmed, 2014; 21:7–12.
17. Patel PK, Patel MA, Vyas BA, Antiurolithiatic activity of saponin rich fraction from the fruits of Solanum xanthocarpum Schrad. and Wendl. (Solanaceae) against ethylene glycol induced urolithiasis in rats. J Ethnopharmacol, 2012; 144:16–70.
18. Devkar RA, Chauhary S, adepu S, Xeviour SK, Chandrakar KS, Setty MM Evaluation of antiurolithiatic and antioxidant potential of Lepidagathis prostrata: a pashanbhed plant, 2015; 1237-1245