Author(s): Shikha Rathi, Pawan Jalwal, Manju Kharb


DOI: 10.52711/0974-360X.2021.00940   

Address: Shikha Rathi*, Pawan Jalwal, Manju Kharb
Shri Baba Mastnath Institute of Pharmaceutical Sciences and Research, Baba Mastnath University, Rohtak, Haryana, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 10,     Year - 2021

Free radicals are generated inside the body during various physiological processes and these free radicals are scavenged by the anti-oxidants present in the body and hence a balance is maintained between these free radicals and anti-oxidants. An imbalance between these free radicals and anti-oxidants lead to a state known as oxidative stress which is one of the main culprits of several diseases. Many medicinal plants are a rich source of anti-oxidants and have been used since time immemorial to combat these diseases by providing various anti-oxidants. The present study was proposed to investigate the phytochemical constituents and anti-oxidant potential of hydro-alcoholic extract of seeds Petroselinum crispum. Phytochemical analysis was performed using different methods and anti-oxidant activity was evaluated by measuring total phenolic content, reducing power assay, hydrogen peroxide (H2O2) scavenging activity, DPPH radical-scavenging activity, superoxide radical-scavenging activity, phosphomolybdenum free radical scavenging activity and nitric oxide radical scavenging activity. The results showed that the hydro-alcoholic extract of seeds P. crispumare a rich source of flavanoids, triterpenoids, phenols, tannins, saponins and amino acids and possess a significant quantity of flavonoids, terpenoids, saponins. It was also revealed that hydro-alcoholic extract of seeds P. crispumpossess a significant anti-oxidant activity as evaluated by using different anti-oxidant assays.

Cite this article:
Shikha Rathi, Pawan Jalwal, Manju Kharb. Evaluation of Phytochemical constituents and Antioxidant potential of hydro-alcoholic extract of seeds of Petroselinum crispum. Research Journal of Pharmacy and Technology 2021; 14(10):5391-6. doi: 10.52711/0974-360X.2021.00940

Shikha Rathi, Pawan Jalwal, Manju Kharb. Evaluation of Phytochemical constituents and Antioxidant potential of hydro-alcoholic extract of seeds of Petroselinum crispum. Research Journal of Pharmacy and Technology 2021; 14(10):5391-6. doi: 10.52711/0974-360X.2021.00940   Available on:

1.    Singh R, Devi S, Gollen R. Role of free radical in atherosclerosis, diabetes and dyslipidaemia: larger-than-life. Diabetes Metab Res Rev. 2015; 31 (2): 113-26.
2.    Funda SP, Keymet T. Free radicals: our enemies or friends? Adv Appl Mol Biol. 2007; 1: 63-69.
3.    Rafieian-Kopaei M, Asgary S, Baradaran A, Rafieian M. Plants antioxidants:from laboratory to clinic. Journal of Nephrology. 2013; 2: 152-153.
4.    Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical Biochemistry. 1999; 269 (2): 337–341.
5.    Zhang H, Chen F, Wang X, Yao HY. Evaluation of antioxidant activity of parsley (Petroselinum crispum) essential oil and identification of its antioxidant constituents. Food Research International. 2006; 39(8): 833-839.
6.    EL-Khalaf M, EL-Toukhy S. Modulatory effect of parsley and vitamin C in ovalbumin induced asthma in mice. Australian Journal of Basic and Applied Sciences. 2013; 7(7): 250-258.
7.    Jabbar A A, Aljanaby J. Antibacterial activity of an aqueous extract of Petroselinum crispum leaves against pathogenic bacteria isolated from patients with burns infections in Al-najaf Governorate. Iraq. Research on Chemical Intermediates. 2012; 39: 3709-3714.
8.    Ozsoy-Sacan O, Yanardag R, Orak H, Ozgey Y, Yarat A, Tunali T. Effects of parsley (Petroselinum crispum) extract versus glibornuride on the liver of streptozotocin induced diabetic rats. Journal of Ethnopharmacology. 2006; 104(1-2): 175-181.
9.    Soliman H A, Eltablawy, N A, Hamed, M S. The ameliorative effect of Petroselinum crispum(parsley) on some diabetes complications. Journal of Medicinal Plants Studies. 2015; 3(4): 92-100.
10.    Rahimi R, Shams-Ardekani M R, Abdollahi M. A review of the efficacy of traditional Iranian medicine for inflammatory bowel disease. World Journal of Gastroenterology. 2010; 16(36): 4504-4514.
11.    Yousofi A, Daneshmandi S, Soleimani N, Bagheri K, Karimi M H. Immunomodulatory effect of Parsley (Petroselinum crispum) essential oil on immune cells: mitogen-activated splenocytes and peritoneal macrophages. Immunopharmacology and Immunotoxicology. 2012; 34(2): 303-308.
12.    Farzaei M H, Abbasabadi Z, Ardekani M R S, Rahimi R, Farzaei F. Parsley: A review of ethnopharmacology, phytochemistry and biological activities. Journal of Traditional and Complementary Medicine. 2013; 33 (6): 815-826.
13.    Irzaie N M, Amabi D, Moazedi A A, Seyyednejad S M. The role of α and β adrenergic receptors in the spasmolytic effects on rat ileum of Petroselinum crispumlatifolium(Parsley). Asian Pacific Journal of Tropical Medicine. 2010; 3(11): 866-870.
14.    Boham B A, Kocipal-Abyazan R. Flavonoids and condensed tannins from leaves of Hawaiian Vac¬cinium vaticulatumand V. calycinum. Pac Sci. 1974; 48: 458-463.
15.    Obadoni B O, Ochuko P O. Phytochemical studies and comparative efficacy of crude extracts of some homeostatic plants in Edo and Delta States of Ni¬geria. Global J Pure Appl Sci. 2001; 8: 203-208. doi:
16.    Harborne J B. Phytochemical methods. 2nd ed. New York. 1984; 3: 100-117.
17.    Bendary E, Francis R R, Ali H M G, Sarwat M I, El Hady S. Antioxidant and structure–activity rela¬tionships (SARs) of some phenolic and anilines compounds. Ann Agric Sci. 2013; 58:173-181. doi:
18.    Benslama A, Harrar A. Free radicals scavenging activity and reducing power of two Algerian Sa¬hara medicinal plants extracts. Int J Herbal Med. 2016; 4(6):158-161.
19.    Maswada H F, Maswada H F. Assessment of to¬tal antioxidant capacity and antiradical scav¬enging activity of three Egyptian wild plants. J Med Sci. 2013; 13(7): 546-554.
20.    Anandjiwala S, Bagul M S, Parabia M, Rajani M. Evaluation of free radical scavenging activity of an ayurvedic formulation, Panchvalkala. Ind J Pharmac Sci. 2008; 70(1): 31.
21.    Marcocci L, Maguire J, Droy-Lefaix M, Packer L. The nitric oxide-scavenging properties of Ginkgo biloba extract EGb 761. Biochemical and Biophysical Research Communications. 1994; 201(2): 748–755.
22.    Trease G E, Evans W C. Textbook of pharmacognosy. 12th ed. London. 1989.
23.    Harbourne J B. Phytochemical methods. A guide to modern techniques of plant analysis. London. 1998.
24.    Vasdev S, Gill V D, SIngal P K. Modulation of oxidaticve stress induces changes in hypertension and atheriosclerosis by antioxidans. Expert Opinion In Clinical Cardiology. 2006; 11: 206-216.
25.    Rafieian-Kopaei M, Asgary S, Adelnia A, Setorki M, Khazaei M, Kazemi S. The effects of corelian cherry on atherosclerosis and atherogenic factors in hypercholersteromic rabbits. J Med Plants Res. 2011; 5: 2670-2676.
26.    Rafieian-Kopaei M, Setorki M, Doaudi M, Baradaran A, Nasri A. Atheriosclerosis:process, indicators, risk factors and new hopes. Int J Prevenative medicine. 2014; 5(8): 927-946.
27.    Prakash A. Antioxidant activity. Med Lab Anal Prog. 2001; 19(2): 1-6.
28.    Meyer H, Bolarinwa A, Wolfram G, Linseisen J. Bioavailability of apigenin from apiin-rich parsley in humans. Annals of Nutrition and Metabolism. 2006; 50(3): 167–172.
29.    Eslavath R, Harikrishna V, Kosuru N,Venkateshwarlu G, Sabat M, Kanakaiah K. Phytochemical Screening and TLC, UV-Spectrophotometer Study of Bougainvillea glabra. Asian J. Pharm. Ana. 2013; 3(3): 83-85.
30.    Kondragunta V K, Karuppuraj V, Perumal K. Antioxidant activity and Folic acid content in indigenous isolates of Ganoderma lucidum. Asian J. Pharm. Ana. 2016; 6(4): 213-215.
31.    Rangasamy P, Hansiya V S, Maheswari P U, Suman T, Geetha N. Phytochemical analysis and evaluation of In vitro anti-oxidant and anti-urolithiatic potential of various fractions of Clitoria ternatea L. Blue flowered leaves. Asian J. Pharm. Ana. 2019; 9(2): 67-76.
32.    Killedar S G, More H N. Screening of Antimicrobial Potential and Phytoconsituents for Different Extracts of Memecylon umbellatum Burm Inflorescences. Asian J. Pharm. Res. 2011; 1(4): 114-118.
33.    Batra N, Jain H, Bairwa R, Bachwani M. Pharmacognostical and preliminary phytochemical investigation of Acorus calamus linn. Asian J. Pharm. Res. 2012; 2(1): 39-42.
34.    Samal P K. Antioxidant activity of Strobilanthes asperrimus in albino rats. Asian J. Pharm. Res. 2013; 3(2): 71-74.
35.    Samal P K. Investigation of Antioxidant Activity of Hibiscus vitifolius leaves. Asian J. Pharm. Res. 2013; 3(4): 215-219.
36.    Wanjari A S. Pharmacognostic Study of White Teak. Asian J. Res. Pharm. Sci. 2015; 5(2): 69-72.
37.    Soumya J, Rajitha G. Synthesis and Biological Evaluation of Naphthylmethyl-1, 3, 4- Oxadiazoles for Anti-oxidant and Anti-bacterial Activities. Asian J. Research Chem. 2015; 8(2): 141-146.
38.    Devmurari V P, Ivani N P. Evaluation of Antioxidant Activity of Artemisia nilagirica. Research J. Pharmacognosy and Phytochemistry. 2010; 2(2): 148-151.

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