Author(s): Krishnaphanisri Ponnekanti, K. Sunitha, S. Ganapaty

Email(s): krishnaphanisri@gmail.com

DOI: 10.52711/0974-360X.2021.00888   

Address: Krishnaphanisri Ponnekanti*, K. Sunitha, S. Ganapaty
GITAM Institute of Pharmacy, GITAM Deemed to be University, Rushikonda, Visakhapatnam - 5303045, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 10,     Year - 2021


ABSTRACT:
A new rapid, accurate, precise and economical reverse phase high performance liquid chromatographic method has been developed and validated for simultaneous estimation of Methylcobalamin, Epalrestat and Pregabalin in bulk and pharmaceutical dosage form. The separation was accomplished utilizing Agilent C18, 150 x 4.6mm, 5? column at a detection wavelength of 210nm utilizing the mobile phase water and acetonitrile 60: 40 v/v at the flow rate of 0.8ml/min and injection volume of 10µl. The total run time was 6.0?min. Validation discovered the method was specific, rapid, accurate, precise, reliable and reproducible. The calibration curve was linear over the concentration range of 0.37 – 2.25µg/ml of Methylcobalamin, 37.5-225µg/ml of Epalrestat and 37.5-225µg/ml of Pregabalin respectively with correlation coefficient of 0.999. The accuracy was determined by recovery studies and was found to be 99.5-100%. The precision of the results stated that the %RSD was <2%. The limits of detection for Methylcobalamin, Epalrestat and Pregabalin were 0.2, 0.9 and 1.2µg/ml, while the limits of quantification were 0.5, 1.5 and 0.9µg/ml respectively. Forced degradation study was carried out under acidic, alkaline, oxidative, photolytic and thermal conditions to prove the stability-indicating ability of the developed HPLC method. The high recovery confirms the suitability of developed method and can be further used in routine analysis.


Cite this article:
Krishnaphanisri Ponnekanti, K. Sunitha, S. Ganapaty. Development and Validation of new RP-HPLC Method for simultaneous Estimation of Methylcobalamin, Epalrestat and Pregabalin in bulk and Pharmaceutical dosage form. Research Journal of Pharmacy and Technology. 2021; 14(10):5097-0. doi: 10.52711/0974-360X.2021.00888

Cite(Electronic):
Krishnaphanisri Ponnekanti, K. Sunitha, S. Ganapaty. Development and Validation of new RP-HPLC Method for simultaneous Estimation of Methylcobalamin, Epalrestat and Pregabalin in bulk and Pharmaceutical dosage form. Research Journal of Pharmacy and Technology. 2021; 14(10):5097-0. doi: 10.52711/0974-360X.2021.00888   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-10-6


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