Author(s): B. Usharani

Email(s): raniushab1@gmail.com

DOI: 10.52711/0974-360X.2021.00992   

Address: B. Usharani
Department of Biochemistry, School of Life Sciences, VISTAS, Chennai.
*Corresponding Author

Published In:   Volume - 14,      Issue - 11,     Year - 2021


ABSTRACT:
Essential oils are extracted from diverse plants and used in medicinal field for diverse conditions. The presence of unique aromatic compounds imparts aroma and medicinal values. Essential oil plays a vital role in pharmaceutical, cosmetics and also acts as rejuvenate. Though there are various plants from which essential oil are extracted, our study focuses on the extraction and comparison of the phytochemicals released from the fresh leaves of Cymbopogon citratus using two different organic solvents; (a). hexane (b) hexane and toluene in the ratio 1:1. Extraction was performed for 16 hours in Soxhlet extractor. The amount of oil obtained was weighed in both extractions and subjected to GC MS for phytochemical analysis. Results revealed that when the solvents hexane and toluene in the ratio 1:1 was used it gave a better yield of 3% from 5 gram of fresh leaves, while hexane yielded only 1.5%. The GC-MS analysis revealed that the compound released from both extractions are of diverse medicinal values and can be used in aromatherapy as rejuvenate. The compounds nerol (2,6 octadienal, 3,7 dimethyl.Z) and geraniol (2,6 octadienal, 3,7 dimethyl) are the common phytochemicals extracted .Nerol and geraniol are geometric isomers. Geraniol is reported as an efficient anticancer agent with additional properties of analgesic, antinflammatory and antiseptic values. Nerol is used as a fragrance. The compound 1 acetyl 4 hydroxy pyrrolidine 2 one is extracted when hexane and toluene were used for extraction in the ratio 1:1. It is reported to be anti-rheumatic and effective in the treatment of hips and joints. Hence it can be concluded that hexane and toluene in the ratio 1:1 can be used for extraction of oil considering its medicinal values and the increased % of extraction rather than using hexane alone.


Cite this article:
B. Usharani. Extraction of Essential oils from Cymbopogon citratus using Organic solvents. Research Journal of Pharmacy and Technology. 2021; 14(11):5709-2. doi: 10.52711/0974-360X.2021.00992

Cite(Electronic):
B. Usharani. Extraction of Essential oils from Cymbopogon citratus using Organic solvents. Research Journal of Pharmacy and Technology. 2021; 14(11):5709-2. doi: 10.52711/0974-360X.2021.00992   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-11-17


REFERENCE:
1.    Cymbopogon citratus. Plants of the world online. Royal Botanic Gardens. Retrieved March 4 2019.
2.    Zhang QW, Lin LG, Ye WC. Techniques for extraction and isolation of natural products: a comprehensive review. Chinese Medicne. 2018;13(20): 1-26.
3.    Vazquez-Briones M.C., Hernandez L.R., Guerrero-Beltran, J.A. Physicochemical and antioxidant properties of Cymbopogoncitratusessential oil. J. Food Res., 2015; 4: 36-45.
4.    Zahraa Abdulelah Al Naqqash, Huda Khalil Al-Bazaz, Fatima Mahdi Salh, Shahad Qusay Ibraheem. GC-Mass and Phytochemical Investigation of Cymbopogon citratus. Research J. Pharm. and Tech 2019; 12(1): 67-73.
5.    Paula D, Luis P, Olivia RP. Aromatherapy in the control of stress and anxiety. 2017;Altern Integr Med 6(248):1-5
6.    Rashidi- Fakari F, Tabatabaeichehr M, Mortazavi H. The effect of aromatherapy by essential oil of orange on anxiety during labor: A randomized clinical trial. 2015; Iran J Nurs Midwifery Res 20(6)661-664
7.    Mohd. Yaqub Khan, Mn-hua Chen. Living with Environmental Stress. Asian J Pharm. Res. 2020; 10(1):31-33.
8.    Ashish Kumar Gupta, Deepak Ganjewala. Purification and Characterization of the 1-Deoxy-D-Xylulose-5-Phosphate Reductoisomerase From Cymbopogon Flexuosus Leaves. Research J. Pharm. and Tech. 8(3): Mar., 2015; Page 320-327.
9.    Chacon MG, Marriott A,Kendrck EG et al. Esterification of geraniol as a strategy for increasing product titre and specificity in engineered Escherichia coli. 2019; Microb Cell fact 18(105). https://doi.org/10.1186/s12934-019-1130-0.
10.    Cho M, So I, Chun JN, Jeon JH. The antitumour effects of geraniol: modulation of cancer hallmark pathways (review) 2016; Int J Oncol. 48: 1772-82.
11.    Liao PC, Yang TS, Chou JC, Chen J, Lee SC et al.  Anti-inflammatory activity of neral and geranial isolated from fruits of Litsea cubeba Lour 2015; Journal of Functional Foods. 19: 248-25.
12.    Hermann G, Steeger D, Klasser M, Wirbitzky J et al. Oxaceprol is a well-tolerated therapy for osteoarthritis with efficacy equivalent to diclofenac 2000;Clin Rheutamol. 19(2):99-104.
13.    Vehelmann A,Hofbauer A, Refior HJ, Massmer K. Oxaceprol, an atypical inhibitor of inflammation, reduces leukocyte adherence in mouse antigen- induced arthritis. 2001:Acta Orthop Scand.72(3):293-298.
14.    Mukhopadhyay K, Ghosh P, Ghotai P, Hazra A, Das AK. Oxaceprol versus tramadol for knee osteoarthritis: A randomized controlled trial 2018: Indian journal of Pharmacology 50(5): 266-272
15.    Ganjewala D. and Gupta, AK. Lemongrass (Cymbopogon flexuosus Steud.) Wats Essential Oils. Recent Progress in Medicinal and Aromatic Plants, Studium Press LLC, USA. 35; 2013:233-274
16.    Sunil KS,, Akk S, Ashka BD, Chitali LR, Naresh S, Balasubramanian  S. GCMS and FTIR analysis on the methanolic extract of Coriandrum sativum leaves 2018; EJPMR 5(8): 454-460
17.    Adesanwo JK, Ajay IS, Ajayi OS, Igbeneghu OA, Mc Donald AG. Identification of chemical constituents and evaluation of the antibacterial activity of Methanol Extract and fractions of the leaf of Melanthera scandens (Schum.et Thonn) Roberty. 2019; Journal of Explotatory research in Pharmacology 4(3): 31-40.
18.    Hiran BL et al. Kinetc and mechanism of oxidation of some substituted benzhydrols by N-bromosuccinimde 2005; Kinetics and catalysis 46(3):334-339.
19.    Ranganadha Reddy , Ravi Kumar V, Priyanka M, Sastry KP. Experimental Studies on Influence of Different Doses of Gypsum on Essential Oil Yield of Lemongrass var. Krishna (Cymbopogon flexuosus). 2014Research J. Pharm. and Tech. 7(4):  439-446.
20.    Ravi Kumar V, Bharath Kumar , Ranganadha Reddy A, Priyanka M, Sastry K.P. Experimental Studies on Influence of Different Doses of Sulphur on Essential Oil Yield of Lemongrass Var. Krishna (Cymbopogon flexuosus). 2014 ;Research J. Pharm. and Tech. 7(8): 856-863.
21.    Zahraa Abdulelah Al Naqqash, Huda Khalil Al-Bazaz, Fatima Mahdi Salh, Shahad Qusay Ibraheem. GC-Mass and Phytochemical Investigation of Cymbopogon citratus. 2019; Research J. Pharm. and Tech  12(1): 67-73.
22.    Sudhanshu, Mittal Sandhya, Rao Nidhi, Menghani Ekta. Phytochemical and Antimicrobial Efficacy of Successive Series of Solanum xanthocarpum. Research J. Pharmacognosy and Phytochemistry 2012; 4(4): 215-219.
23.    Priya Narkar, Savanta V Raut. Study of Antibiofilm Activity of Methanolic Extract and Oil of Cymbopogon citratus and its other Applications. 2014; Res. J. Pharmacognosy & Phytochem. 6(4): 160-169.
24.    Anil G Markandeya, Narayan P Firke, Suneeti S Gore, Sunita Salunke Gawali, Shirish S Pingale. Antibacterial Activity of Celocia argentea Leaves Extract in Organic Solvents. 2014; Research Journal of Pharmacology and Pharmacodynamics. 6(2): 79-81.
25.    MD Wandhare, UA Deokate, SS Khadabadi, SP Hadke, HA Sawarkar. Comparative Estimation of Curcumin Content from Marketed Herbal Anti Rheumatic Tablets Formulation2009; . Asian J. Research Chem. 2(3):340-343.
26.    Merlin NJ, Parthasarathy V, Manavalan R, Devi P, Meera R. Phyto-Physico chemical evaluation, Anti-Inflammatory and Anti microbial activities of Aerial parts of Gmelina asiatica. 2009; Asian J. Research Chem. 2(1): 76-82.
27.    J Ramamoorthy, S Venkataraman, R Meera, N Chidambaranathan, P Devi Devisree. Phyto-Physico Chemical Investigation, Anti-inflammatory and Antimicrobial Activities of Pollianthes tuberosa Linn. 2009;Research J. Pharm. and Tech.2 (4): 738-742.
28.    Mukesh Kumar Singh, A. Prathapan, Kushagra Nagori, S. Ishwarya, K.G Raghu. Cytotoxic and Antimicrobial Activity of Methanolic Extract of Boerhaavia diffusa L. 2010; Research J. Pharm. and Tech.3 (4):  1061-1063.

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