Author(s):
Asish Bhaumik, Samaresh Datta, Susmita Datta, Radheshyam Samanta, B. D. Tripathi
Email(s):
bhaumik.asish@gmail.com
DOI:
10.52711/0974-360X.2021.01041 
Address:
Asish Bhaumik1*, Samaresh Datta1, Susmita Datta1, Radheshyam Samanta2, B. D. Tripathi3
1Department of Pharmaceutical Chemistry, Narayan Institute of Pharmacy, Gopal Narayan Singh University, Jamuhar - 821305, Bihar, India.
2Department of Pharmaceutics, Narayan Institute of Pharmacy, Gopal Narayan Singh University, Jamuhar - 821305, Bihar, India.
3Department of Pharmacology, Narayan Institute of Pharmacy, Gopal Narayan Singh University, Jamuhar - 821305, Bihar, India.
*Corresponding Author
Published In:
Volume - 14,
Issue - 11,
Year - 2021
ABSTRACT:
The main aim and objective of the present research work was the isolation of some novel bioflavanoids from methanolic extract of peels of sweet lime (MEE-PSWL) and evaluation of in vitro anticancer activity followed by molecular docking against target protein topoisomerase II. The extraction was done by reflux condensation method and preliminary phytochemical screening of MEE-PSWL was carried out for the evaluation of bioactive molecules, the bioflavanoids present in MEE-PSWL was confirmed by spectral analysis such as ESI-MS-MS and FTIR. Molecular docking of isolated compounds was carried out against target protein Topoisomerase II with PDB id 1ZXM by Auto dock program and the best dock pose was selected based on the interaction study analysis. In vitro anticancer activity MEE-PSWL was evaluated by SRB assay toward human colon adenocarcinoma cell line SW620 and the test was carried out at different concentrations. A preliminary screening displayed that the MEE-PSWL was able to inhibit the proliferation of more than 60% of human colon adenocarcinoma SW620 cells. The maximum growth of inhibition was found to be at 125µg/ml (IC50 4.5µg/ml) and the standard drug doxorubicin was found to inhibit the maximum proliferation at concentration 75µg/ml (IC50 3µg/ml).
Cite this article:
Asish Bhaumik, Samaresh Datta, Susmita Datta, Radheshyam Samanta, B. D. Tripathi. Extraction and Isolation of Phenolic compounds from Sweet lime and Evaluation of Anticancer potentiality followed by Molecular docking against Topoisomerase II. Research Journal of Pharmacy and Technology. 2021; 14(11):5993-7. doi: 10.52711/0974-360X.2021.01041
Cite(Electronic):
Asish Bhaumik, Samaresh Datta, Susmita Datta, Radheshyam Samanta, B. D. Tripathi. Extraction and Isolation of Phenolic compounds from Sweet lime and Evaluation of Anticancer potentiality followed by Molecular docking against Topoisomerase II. Research Journal of Pharmacy and Technology. 2021; 14(11):5993-7. doi: 10.52711/0974-360X.2021.01041 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-11-67
REFERENCES:
1. Porcher, Michel H. et al. Multilingual Multiscript Plant Name Database (M.M.P.N.D): Sorting Citrus Names. The University of Melbourne. 1995.
2. Raj. K. Bansal. Laboratory Manual of Organic Chemistry, New Age International Publisher January, 2008.
3. P. K Sharma and P. C Dandiya. Biochemistry and Clinical Pathology, January, 2014.
4. Dr. G. Devala Rao. A Textbook of Applied Biochemistry & Clinical Pathology, 2006
5. Jaswant Kaur. PV Chemistry of Natural Products, 2010.
6. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry. 1999; 64:555-559.
7. Tzulker R, Glazer I, Bar-Ilan I, Holland D, Aviram M, Amir R. Antioxidant activity, polyphenol content and related compounds in different fruit juices and homogenates prepared from 29 different pomegranate accessions. J Agri Food Chem. 2007;55; 9559–9570.
8. Borges G, Mullen W, Crozier A. Comparison of the polyphenolic composition and antioxidant activity of European commercial fruit juices. Food Function. 2010; 1: 73-83.
9. Master RW. Animal Cell Culture, cytotoxicity and viability assay, 3rd ed. 2000, p. 202-203.