Author(s): P. Kalaivani, D. Kavitha, P. Amudha


DOI: 10.52711/0974-360X.2021.01073   

Address: P. Kalaivani 1, D. Kavitha1,2, P. Amudha1*
1Department of Biochemistry, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai, Tamilnadu - 600117, India.
2GIET School of Pharmacy, NH-16,Chaitanya knowledge City, Rajamundry, Andhra Pradesh - 533296, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 12,     Year - 2021

Seagrass are the marine flowering plants found mainly in clear, shallow estuaries and coastal waters. In all temperate and trophical region seagrasses grow both internally and subtidally. One such seagrass namely Syringodium isoetifolium has many medicinal properities. This seagrass have most promising pharmacological activities which may include anti-inflammatory, anticancer, antidiarrheal, antihaemorrhoidal activities. This study is focussed on the phytochemical evaluation and in vitro antioxidant activity of aqueous, ethanol and hydroalcoholic extract of Syringodium isoetifolium. The qualitative analysis of Syringodium isoetifolium shows the presence of tannin, saponin, flavonoids, steroids, terpenoids, alkaloids, anthraquinone, polyphenol and coumarin. In all the three extracts only ethanol shows the high concentration of phytocompounds. Emodins, glycoside and anthocyanin were found to be absent in all the three extracts. Quantitative analysis of total phenol, flavonoid, saponin and tannin were found to be 193.10 ±13.52, 106.11 ± 7.42, 52.96 ± 3.64 and 81.30 ± 5.69. Superoxide anion radical, Nitric oxide and Hydroxy radical scavenging assay showed that Syringodium isoetifolium was an excellent scavenger of these radicals. These results are an indication of the potent antioxidant property of the extract and may be responsible for some of the therapeutic uses of Syringodium isoetifolium.

Cite this article:
P. Kalaivani, D. Kavitha, P. Amudha. In vitro Antioxidant activity and Phytochemical composition of Syringodium isoetifolium. Research Journal of Pharmacy and Technology. 2021; 14(12):6201-6. doi: 10.52711/0974-360X.2021.01073

P. Kalaivani, D. Kavitha, P. Amudha. In vitro Antioxidant activity and Phytochemical composition of Syringodium isoetifolium. Research Journal of Pharmacy and Technology. 2021; 14(12):6201-6. doi: 10.52711/0974-360X.2021.01073   Available on:

1.    Anand, Vijay, Bharathi and Vadivelu. Chemical Characterization from GC-MS studies of ethanolic extract of Macrotyloma uniflorum. Research Journal of Pharmacy and Technology. 2016; 9(3).
2.    Nikhat F, D. Satyanarayana and Subramaniyan EVS. Phytochemistry and Pharmacology of Indian Medicinal Plants Zizyphus Mauritiana Lamk. Research Journal of Pharmacognosy and Phytochemistry. 2009; 1(1): 5-10.
3.    Kale PM. A Review Article on Euphorbia hirta uses and pharmacological activities. Asian J. Res. Pharm. Sci. 2016; 6(3): 141-145.
4.    Maharaja P, Sengottuvel T, Aarthi A, Gopalasatheeskumar K. Review on Antioxidant and Hepatoprotective activity of Medicinal plants against Paracetamol induced animal model. Research Journal of Pharmacognosy and Phytochemistry. 2020; 12(2): 114-119.
5.    V.Bharathi, A. Vijaya Anand. Chemical characterization from GC-MS studies of ethanolic extract of Macrotyloma uniflorum.Research Journal of Pharmacy and Technology. 2016; 9(3): 238-240.
6.    Ali Esmail, Al-Snafi. Therapeutic properities of Medicinal plants: A Review of their Immunological Effects. Asian Journal of Pharmaceutical Research. 2015; 5(3): 208-216.
7.    Nachiket S Dighe, Shasikant R Pattan, Deepak S Musmade, Abhijeet N Merekar, Manisha S Kedar, Deepak K Thakur, Pratik V Patel. Recent Synthesis of Marine Natural Products with Anticancer Activity: An Overview. Research Journal of Science and Technology. 2009; 1(2): 63-70.
8.    Robert J.Orth, Tim J.B. Carruthers, William C.Dennison, Carlos M. Duarte, James W.Fourqurean, Kenneth L.Heck, A.Randall Hughes, Gary A. Kendrick, W. Judson Kenworthy, Suzanne Olyarnik, Frederick T.Short, Michelle Waycott and Susan L. Williams. A Global Crisis for Seagrass Ecosystem. Bioscience. 2006; 56(12): 987-996     
9.    Peter Hanington, Kimberley Hunnam, Ron Johnstone. Widespread loss of the seagrass Syringodium isoetifolium after a major flood event in Moreton Bay, Australia: Implications for benthic processes. Aquatic Botany. 2015;120: 244-250.
10.    Short .F.T, Wyllie- Echeverria S. Natural and human- induced disturbance of seagrasses. Environmental Conservation. 1996; 23(1): 17-27.
11.    Lee K S,Park S R, Kim Y.K. Effects of irradiance, temperature and nutrientson growth dynamics of seagrasses: A review. Journal of Experimental Marine Biology and Ecology. 2007; 350(1-2): 144-175.
12.    Aswathi Elizabeth Mani, Velammal Aiyamperumal and Jamila Patterson. Phytochemicals of the Seagrass Syringodium isoetifolium and its Antibacterial and Inseticidal Activities.European Journal of Biological Sciences. 2012: 63-67.
13.    Girija K, Parthiban C, Hemalatha A, Saranya C, Antharaman P. Evaluation of antioxidant activities and preliminary Phytochemical analysis of seagrass Halodule pinifolia, Halophila ovalis and Syringodium isoetifolium. The Journal of Phytochemistry. 2013; 114: 181-187.
14.    Kalaivani P, KavithaD, Vanitha V. A Review on Phytochemical and Pharmacological activities of Syringodium isoetifolium. International Journal of Research in Pharmaceutical Sciences. 2020; 11(1): 207-214.
15.    Jadhav Sameer S, Salunkhe Vijay R and Magdum Chandrakant S. Daily Consumption of Antioxidants:-Prevention of Disease is better than Cure. Asian J. Pharm. Res. 2013; 3(1): 33-39.
16.    Miss.Sawant Rutuja, Miss. Baghkar Aloka, Miss. Jagtap Sanjukta, Miss Harad Lina, Miss. Chavan Anagha, Mr. Khan Nilofar.A, Mr. Yevale Rupali P, Dr. Kale Mohan. A Review on Herbs in Anticancer. Asian Journal of Research in Pharmaceutical Science. 2018; 8(4): 179-184.
17.    Vignesh Balaji E, Tamil Selvan A, Srinivasan A and Nandhini S. Punica granatum root (s): Phytocompounds analysis, Anti-oxidant and Anti-microbial activity. Asian J. Pharm. Ana. 2019; 9(3): 123-127.
18.    Vanitha Varadharajan, Umadevi Kumba Janarthanan and Vijayalakshmi Krishnamoorthy.  Physicochemical, Phytochemical screening and profiling of Secondary metabolites of Annona squamosa leaf extract. World Journal of Pharmaceutical Research. 2012; 1(4): 1143-1164.
19.    Sofowara, A. Medicinal plants and Traditional medicine in Africa. Spectrum Books Ltd, Ibadan, Nigeria. 1993; 289.
20.    Trease, G. E., and Evans, W. C. Phenols and Phenolic glycosides. In: Textbook of Pharmacognosy. (12th edition.). Balliese, Tindall and Co Publishers, London, 1989; 343-383.
21.    Harborne, J. B. Phytochemical methods, London. Chapman and Hall, Ltd. 1973; 49-188.
22.    Kim DO, Jeong SW, Lee CY. Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem. 2003; 81: 321–326
23.    Katasani D. Phytochemical screening, quantitative estimation of total phenolic, flavanoids and antimicrobial evaluation of Trachyspermum ammi. J Atoms and Molecules. 2011; 1: 1–8.
24.    Bajaj K. L and Devsharma A. K.  A Colorimetric Method for the Determination of Tannins in Tea. Mikrochimica Acta [Wien] 1977 II: 249-253
25.    Hiai, S., Oura, H. and Nakajima, T. Color reactions of some sapogenins and saponins with vanillin and sulphuric acid. Planta Medica. 1976; 29: 116-122.
26.    Shimada, K., Fujikawa, K., Yahara, K., and Nakamura, T. Antioxidative properties of xanthum on the autoxidation of soybean oil in cyclodextrin emulsion. Journal of Agricultural and Food Chemistry. 1992; 40: 945–948.
27.    Liu, F., Ooi, V.E.C. and Chang, S.T. Free radical scavenging activity of mushroom polysaccharide extracts. Life Science. 1997; 60: 763-771.
28.    Yu, T. W., Xu, M., and Dashwood, R. H. Antimutagenic activity of spearmint. Environ. Mol. Mutagen. 2004; 44: 387–393.
29.    Garrat, D. C. The quantitative analysis of drugs, Japan: Chapman and Hall. 1964; 3: 456-458.
30.    Prieto, P., Pineda, M., and Aguilar, M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex  Speci.c application to the determination of vitamin E. Analytical Biochemistry. 1999; 269: 337-341.
31.    Praveen kumar ashok, Kumud Upadhayaya. Tannina are Astringent. Journal of Pharmacognosy and Phytochemistry. 2012; 1(3): 45-50.
32.    Cosman Domettila, Josepj Joselin and Solomon Jeeva. Phytochemical analysis of some south Indian seaweeds. Journal of Chemical and Pharmaceutical Research. 2013; 5(4): 275-278.
33.    Pier- Giorgio Pietta. Flavonoids as Antioxidants. Journal of natural Products. 2000; 63(7): 1035-1042.
34.    Wenying Ren, Zhenhua Qiao, Honwei wang, Lei Zhu, Li Zhang. Flavonoids: Promising Anticancer agents. Medicinal Research Reviews. 2003; 23: 519-534.
35.    Robert J Williams, Jeremy P.E Spencer, Catherine Rice-Evans. Flavonoids: Antioxidants or Signalling molecules. Free Radical Biology and Medicine. 2004; 36(7): 838-849.
36.    Robak J, Gryglewski RJ. Bioactivity of flavonoids. Polish Journal of Pharmacology. 1996; 48(6): 555-564.
37.    J.Grabmann. Terpenoids as Plant Antioxidants. Vitamins and Hormones. 2005; 72: 505-535.
38.    Dhan Prakash, Samiksha Suri, Garima Upadhyay and Brahma N.Singh. Total phenol, antioxidant and free radical scavenging activities of some medicinal plants. International Journal of Food science and Nutrition. 2007; 58(1): 18-28.
39.    Mohammad Reza Shams Ardekani, Mahanaz Khanavi, Mannan Hajimahmoodi, Maryam Jahangiri and Abbas Hadjiakhoondi. Comparison of Antioxidant activity and Total phenol contents of some Date Seed Varieties from Iran. Iranian Journal of Pharmaceutical Research.2010; 9(2): 141-146.
40.    Akiyama H, Fujii K, Yamasaki O, Oono T and Iwatsuki K. Antibacterial action of several tannins against Staphylococcus aureus. 2001; 48(4): 487-491.
41.    N.Yuvaraj, P.Kanmani, R.Sathishkumar, A.Paari, V.Pattukumar and V.Arul. Seagrass as a potential source of natural antioxidant and anti-inflammatory agents. Pharmaceutical Biology. 2012; 50(4): 458-467.
42.    Esra  Birben, Umit Murat Sahiner, Cansin Sackesen, Serpil Erzurum, Omer Kalayci. Oxidative Stress and Antioxidant Defense. World Allergy Organisation Journal. 2012; 5: 9-19.
43.    Gayathri Gunalan, Neelima Myla and R.Balabhaskar. Invitro Antioxidant Analysis of Selected Coffee Bean Varieties. Journal of Chemical and Pharmaceutical Research. 2012; 4(4): 2136-2132.
44.    Vanacker, S.A.B.E,Tromp, M.N.J.L, Haenen, G.R.M.M, Vandervijgh, W.J.F, Bast. A. Flavonoids as Scavengers of Nitric Oxide Radical. Biochemical and Biophysical Research Communications. 1995; 214(3): 755-759.
45.    Steven R. Vincent. Nitric Oxide: A radical neurotransmitter in the central nervous system. Progress in Neurobiology. 1994; 42(1): 129-160.

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