Author(s): M.S. Sindhu, M. Poonkothai


DOI: 10.52711/0974-360X.2021.01103   

Address: M.S. Sindhu, M. Poonkothai
Department of Zoology, Avinashilingam Institute for Home Science and Higher Education for Women Coimbatore – 641 043, Tamil Nadu, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 12,     Year - 2021

The present study is to evaluate the phytochemical constituents and antimicrobial activity of Plectranthus amboinicus leaf extract using petroleum ether, methanol and water as solvents. The antimicrobial activity of P. amboinicus leaf extracts were examined against the selected bacterial and fungal isolates namely Escherichia coli, Staphylococcus aureus and Aspergillus niger, Candida albicans using agar well diffusion method. FT –IR analysis was performed to identify the presence of functional groups in the methanol extract. The results of the study revealed the presence of distinctive active secondary metabolites in the tested leaf extract. The results revealed that the methanol extract exhibited maximum antimicrobial activity against the tested bacterial and fungal isolates when compared with aqueous and petroleum ether extracts. The FT-IR analysis reveals the presence of functional groups such as hydroxyl, amines, alkynes, ketones and carboxylic acid in the methanolic extract of P. amboinicus. Thus, P. amboinicus leaves are effective against the selected microbes and recommends that the plant derived phytochemicals are comparatively safer than synthetic alternative, thereby contributing insightful remedial benefits for the treatment of diseases.

Cite this article:
M.S. Sindhu, M. Poonkothai. Phytochemical and antimicrobial analyses of Plectranthus amboinicus leaf extracts. Research Journal of Pharmacy and Technology. 2021; 14(12):6379-4. doi: 10.52711/0974-360X.2021.01103

M.S. Sindhu, M. Poonkothai. Phytochemical and antimicrobial analyses of Plectranthus amboinicus leaf extracts. Research Journal of Pharmacy and Technology. 2021; 14(12):6379-4. doi: 10.52711/0974-360X.2021.01103   Available on:

1.    Kapila A. The challenge of antibiotic resistance: Need to Contemplate. Indian Journal of Medical Research. 2005; 121: 83–91.
2.    Runyoro DK, Matee MI, Ngassapa OD, Joseph CN and Mbwambo, ZH. Screening of Tanzanian medicinal plants for anti‐candida activity. BMC Complementary and Alternative Medicine. 2006; 6(11).
3.    Chandrappa MS, Harsha R, Dinesha R and Gowda TSS. Antibacterial activity of Coleus aromaticus leaves. International Journal of Pharmacy and Pharmaceutical Sciences. 2010; 2(3): 63-64.
4.    Cragg GM and Newman DJ. Biodiversity: A continuing source of novel drug leads. Pure Applied Chemistry. 2005; 77: 7–24.
5.    Lam KS. New aspects of natural products in drug discovery. Trends in Microbiology. 2007; 15: 279–289.
6.    Arumugam G, Swamy MK and Sinniah UR. Plectranthus amboinicus (Lour.) Spreng: Botanical, phytochemical, pharmacological and nutritional significance. Molecules. 2016; 21(4): 369.
7.    Obidoa, Onyechi, Joshua, Elijah P, Egemole, John C and Adachukwu I. Phytochemical analysis of aqueous flower extract of Hibiscus sadariffa (Zobo Flower). Research Journal of Pharmacognosy and Phytochemistry. 2011; 3(4): 169-173.
8.    Abirami S, Nishanthini K, Poonkothai, M.Antimicrobial activity and phytochemical screening of the leaf extracts of Eucalyptus globulus. International Journal of Current Pharmaceutical Research. 2017; 9(5): 85-89.
9.    Bauer AW, Kirby WMM, Sherris JC and Turck M. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology. 1966; 45:493 496.
10.    Tiwari P. Antimicrobial activity of amritarishta prepared by traditional and modern methods. Asian Journal of Pharmaceutical Research. 2014; 4(2):114-116.
11.    Kumara Swamy M, Pokharen N, Dahal S. and Anuradha M. Phytochemical and antimicrobial studies of leaf extract of Euphorbia nerifolia. Journal of Medicinal Plant Research. 2011; 5: 5785–5788.
12.    Swamy MK and Sinniah UR..A comprehensive review on the phytochemical constituents and pharmacological activities of Pogostemon cablin. Benth.: An aromatic medicinal plant of industrial importance. Molecules. 2015; 20: 8521–8547.
13.    Knab A, McKinney H and Marta MK. 2008. Isolation of the compounds in Mentha piperita, Mentha spicata and Coleus amboinicus; Abstracts of Papers, 238th ACS National Meeting; Washington, DC, USA, 2008; pp. 16–20.
14.    Singh G, Singh OP, Prasad YR, Lamposona MP and Catalan C. Studies on essential oils. Part 33. Chemical and insecticidal investigations on leaf oil of Coleus amboinicus (Lour). Flavour and Fragrance Journal. 2002; 17: 440–442.
15.    Senthilkumar A and Venkatesalu V. Chemical composition and larvicidal activity of the essential oil of Plectranthus amboinicus (Lour.) Spreng against Anopheles stephensi: A malarial vector mosquito. Parasitology Research. 2010; 107: 1275–1278.
16.    Castillo RAM and Gonzalez VP. Plecthranthus amboinicus (Lour.) Spreng. Revista Cubana de Plantas Medicinales. 1999; 4: 110–115.
17.    Roshan P, Naveen M, Manjul PS, Gulzar A, Anita S. and Sudarshan S. Plectranthus amboinicus (Lour) Spreng: An overview. The Pharma Research. 2010; 4: 1–15.
18.    18.Khare RS, Banerjee S and Kundu KK. Coleus aromatics Benth. A nutritive medicinal plant of potential therapeutic value. International Journal of Pharma and Biosciences. 2011; 2: 488–500.
19.    Can Baser KH. Biological and pharmacological activities of carvacrol and carvacrol bearing essential oils. Current Pharmaceutical Design. 2008; 14: 3106–3119.
20.    Abriyani E and Fikayuniar L. Screening phytochemical, antioxidant activity and vitamin C assay from Bungo perak-perak (Begonia versicolar Irmsch) leaves. Asian Journal of Pharmaceutical Research. 2020; 10(3):183-187.
21.    Amutha K and Doss DVA. In vitro antioxidant activity of ethanolic extract of Barleria cristata L. leaves. Research Journal of Pharmacognosy and Phytochemistry. 2009; 1(3): 209-212.
22.    Bhatt P and Negi PS. Antioxidant and antibacterial activities in the leaf extracts of Indian borage (Plectranthus amboinicus). Food and Nutrition Sciences. 2012; 3: 146-152.
23.    Chandra B, Palni LMS and Nandi SK. Propagation and Conservation of Picrorhiza kurrooa Royle ex Benth.: An Endangered Himalayan Medicinal Herb of High Commercial Value. Biodiversity and Conservation. 2006; 15(7): 2325-2338.
24.    Pritima RA and Pandian RS. Antimicrobial activity of Coleus aromaticus (Benth) against microbes of reproductive tract infections among women. African Journal of Infectious Diseases. 2007; 1(1): 18-24.
25.    Deena MJ, Sreeranjini K and Thoppil JE. Antimicrobial screening of essential oils of Coleus aromaticus and Coleus zeylanicus. International Journal of Aromatherapy. 2002; 12(2): 105-107.
26.    Mohite SA, Shah RR and Patel NR. Antimicrobial activity of leaves extracts of Jatropha curcas. Asian Journal of Pharmaceutical Research. 2018; 8(1):17-20.
27.    Mohite SA, Shah RR and Patel NR. Antimicrobial activity of leaves extracts of Passiflora foetida. Asian Journal of Pharmaceutical Research. 2018; 8(1):17-20.
28.    Mohite SA, Shah RR and Patel NR. Antimicrobial activity of leaves extracts of Cassia tora. Research Journal of Pharmaceutical Dosage Forms and Technology. 2018; 10(1): 10-12.
29.    Edrah S, Osela E and Kumar A. Preliminary phytochemical and antibacterial studies of Convolvulus arvensis and Thymus capitatus plants extracts. Research Journal of Pharmacognosy and Phytochemistry. 2013; 5(5): 220-223.
30.    31.Premakumari KB, Siddiqua A, Banu S, Josephine J, Jenita L and Raj B. Comparative antimicrobial studies of methanolic extract of Muntingia calabura, Basella alba and Basella rubra leaves. Research J. Pharmacognosy and Phytochemistry 2010; 2(3): 246-248.
31.    Lydia E, John S, Mohammed R and Sivapriya T. Investigation on the phytochemicals present in the fruit peel of Carica papaya and evaluation of its antioxidant and antimicrobial property. Research Journal of Pharmacognosy and Phytochemistry. 2016; 8(4): 217-222.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

56th percentile
Powered by  Scopus

SCImago Journal & Country Rank

Recent Articles


Not Available