Author(s): Himshikha Yadav, Sushil Kumar


DOI: 10.52711/0974-360X.2021.01129   

Address: Himshikha Yadav1, Sushil Kumar2*
1Department of Botany, V.R.A.L. Govt. Mahila Degree College, Bareilly - 243 005, India.
2Department of Zoology, Govt PG College, Bisalpur, Pilibhit-262201.
*Corresponding Author

Published In:   Volume - 14,      Issue - 12,     Year - 2021

The leaves of Aloe barbadensis are used in traditional and modern systems of medicine. The aim of this study was to evaluate the cytotoxic potentialities of aqueous solution of Aloe barbadensis leaves on Trigonella foenum-graecum root tip meristem cells using a cytogenetic approach. Treatments with various concentrations of Aloe barbadensis leaf extract to Trigonella foenum-graecum root meristem cells showed mito-inhibition and induced several chromosomal aberrations as chromsomal breakage, fragmentation, scattering, stickiness etc.

Cite this article:
Himshikha Yadav, Sushil Kumar. An assessment of cytotoxic potentiality of Aloe barbadensis on the root meristem cells of Trigonella foenum-graecum L. Research Journal of Pharmacy and Technology. 2021; 14(12):6531-5. doi: 10.52711/0974-360X.2021.01129

Himshikha Yadav, Sushil Kumar. An assessment of cytotoxic potentiality of Aloe barbadensis on the root meristem cells of Trigonella foenum-graecum L. Research Journal of Pharmacy and Technology. 2021; 14(12):6531-5. doi: 10.52711/0974-360X.2021.01129   Available on:

1.    Avila H., Rivero J., Herrera F and Fraile G. Cytotoxicity of a low molecular weight fraction from Aloe vera (Aloe barbadensis, Miller) gel. Toxicon. 1997; 35(9): 1423-1430.
2.    Carvalho NC, Guedes SAG, Albuquerque-Júnior RLC, de Albuquerque DS, de Souza Araújo AA, Paranhos LR, Camargo SEA,  Ribeiro MAG. Analysis of Aloe vera cytotoxicity and genotoxicity associated with endodontic medication and laser photobiomodulation. J Photochem Photobiol B. 2018 Jan; 178:348-354. doi: 10.1016/j.jphotobiol.2017.11.027. Epub 2017 Nov 22.
3.    Kumar A., Mahajan A.2, Begum Z. Phytochemical Screening and In vitro Study of Free Radical Scavenging Activity of Flavonoids of Aloe vera. Research J. Pharm. and Tech 2020; 13(2):593-598.
4.    Bharti Ahirwar, Dheeraj Ahirwar. In vivo and in vitro investigation of cytotoxic and antitumor activities of polyphenolic leaf extract of Hibiscus sabdariffa against breast cancer cell lines. Research J. Pharm. and Tech 2019; 13(2):615-620.
5.    Zeinab A. Salama, Ahmed M. Aboul-Enein, Alaa A. Gaafar, Mohsen S. Asker, Hanan F. Aly, Habiba A. Ahmed. In-vitro Antioxidant, Antimicrobial and Anticancer Activities of Banana leaves (Musa acuminata) and Olive leaves (Olea europaea L.) as by-products. Research J. Pharm. And Tech 2020; 13(2):687-696.
6.    Mousa M. Mitotic inhibition and chromosomal aberrations induced by some herbicides in root tips of A. cepa. Egypt. J. Genet. Cytol.1982; 11, 193-207.
7.    Roy A.K. and Bhattacharya G.N.  Effects of waste water effluents of fertilizer industry on root mersitem cells of Vicia faba L. Perspectives in Cytology and Genetics. 1989; 6765-6768
8.    Shehab A.S. and Adam Z.M. Cytological effects of medicinal plants in Qatar. III. Mitotic effect of water extract of Anastatica hierochuntica L. on Allium cepa. Cytologia.1983; 48: 343-348.
9.    Sobita K and Bhagirath T. Effects of some medicinal plant extracts on Vicia faba root tip chromosomes. Caryologia 2005; 58(3):255-261.
10.    Nandi S. and  Dalal T.  Cytological Changes in Fenugreek (L.) under Water Deficit Stress Induced by PEG-6000. International Journal of Science and Research (IJSR) 2014; 3(11):715-717
11.    Yadav H, Kumar S. An Assessment of Cytotoxic Potentiality of Plantago ovata Husk Aqueous Suspension on the Root Meristem Cells of Vicia faba L. Research J. Pharm. and Tech. 2017; 10(7): 2053-2057.
12.    Devi SR and Thoppil JE. Cytotoxic studies and phytochemical analysis of Orthosiphon thymiflorus (Roth) Sleesen. International Journal of Pharmacy and Pharmaceutical Sciences 2016; 8 (2):249-255.
13.    Saito F and Nakano M.  Partial synchronization of cell division and micronucleation in suspension-cultured cells of hydroxyurea and various spindle toxins. Breeding Sci. 2001; 51, 285-291.
14.    Asthana M and Kumar A. Dose response of Viola odorata on meiotic and mitotic chromosomes of Vicia faba. British Journal of Pharmaceutical Res. 2014; 4(4), 522-540.
15.    Campos J M S, Davide L C, Soares G L G and Vicinia L F.  Mutagenic effects due to allelopathic action of ferns (Gleicheniaceae) extracts. Allelopathy Journal 2008; 22, 143-152.
16.    Onyenwe C. N. (1983) Cytological effects of seed extracts of Abrus procatorious on the mitosis of Allium cepa and the effect of root extract of Boerhaavia diffusa on mitosis of Crassocephallum biafrae. University of Port Harcourt, Port Harcourt.
17.    17.Marzougui N., Anissa Boubaya A.,  Thabti I. and Elfalleh W. Polyploidy induction of Tunisian Trigonella foenum-greaum L. populations. African journal of biotechnology.2011; 10(43):8570-8577•
18.    N. Astalakshmi, R. Sundara Ganapathy. In vitro cytotoxicity studies on methanolic leaf extract of Mussaenda erythrophylla Schumach. and Thonn. Research J. Pharm. and Tech 2020; 13(2):831-839.
19.    Karthiga Muralidharan, Punnagai Kumaravelu, Darling Chellathai David. Cytotoxic effect of Ethanolic extracts of Andrographis echioides in human colon cancer cell Lines Via Apoptosis. Research J. Pharm. and Tech 2020; 13(2):871-876.
20.    Rathi M.A., Meenakshi P., Guru Kumar D., Arul Raj C., Sunitha M., Gopalakrishnan V.K. Leaves of Spermacoce hispida as a Novel Cancer Therapeutic – An In Vitro Study. Research J. Pharm. and Tech. 4(8): August 2011; Page 1288-1291.
21.    Kaniz Fatima Urmi, Ashraf Uddin Chowdhury, Farhana Diba, Khandaker Ashfaqur Rahman, Razibul Habib, Kaiser Hamid. Evaluation of Antioxidant and Cytotoxic Potential of Different Extracts from the Leaves of Aegle marmelos L. Research J. Pharm. and Tech. 6(4): April 2013; 384-387.
22.    Jinsu Mathew, Kavita Maria Arora, Akash Mazumdar, Gaurav Kumar, Loganathan Karthik, Kokati Venkata Bhaskara Rao. Evaluation of phytochemical composition and antioxidant activity of aqueous extract of Barleria mysorensis and Furcraea foetida leaves. Research J. Pharm. and Tech. 5(12): Dec. 2012;1503-1508.
23.    Mukesh Kumar Singh, A. Prathapan, Kushagra Nagori, S. Ishwarya, K.G Raghu. Cytotoxic and Antimicrobial Activity of Methanolic Extract of Boerhaavia diffusa L. Research J. Pharm. and Tech.3 (4): Oct.-Dec.2010; 1061-1063.
24.    Ma. Te-Hsiu. Vicia cytogenetic tests for environmental mutagens. A report of the U.S. Environmental Protection Agency Gene- Tox Program. Mutation Res. 1982; 99: 257-271.
25.    Stoltz D.R.; Khera K.S.; Bendall R. and Gunner, S.W.  Cytogenetic studies with cyclomate and related compounds. Science. 1970; 167: 1501-1502.
26.    Grant W.F. Chromosome aberrations in plants as a monitoring system. Environ. Health Perspect. 1978; 27: 37-43.
27.    Wolff S. Induced chromosome variation. In: Chromosome Today eds. Bannett, M. Bobrow, M. and Hewitt, G. M. George Allen and Unwin, London.1981; 7: 226-241.
28.    Swierenga S.H.H.; Heddle J.A.; Sigal E.A.; Gilman J.P.W.; Brillinger R.L.; Douglas G.R.  and Nestmann E.R.  Recommended protocols based on a survey of current practice in genotoxicity testing laboratories IV. Chromosome aberration and sister chromatid exchange in Chinese hamster ovary V79 Chinese hamster lung and human lymphocyte cultures. Mutation Res. 1991; 246(2): 301-322.

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