Mangestuti Agil, Herra Studiawan, Neny Purwitasari
Mangestuti Agil*, Herra Studiawan, Neny Purwitasari
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia.
Volume - 14,
Issue - 12,
Year - 2021
Tuberculosis is still a pandemic disease in Indonesia. People in Madura Island, Indonesia, usually use the rhizome of Merremia mammosa Hall as anti-tuberculosis. In this study, the antibacterial activity of terpenoid fractions of Merremia mammosa rhizome against Mycobacterium tuberculosis has been studied using agar dilution method at a concentration of 200-25 µg/mL, incubated for 21 days in 7H10 Midlebrook medium. N-hexane extract of Merremia mammosa has been fractionated using ethyl acetate. The ethyl acetate fraction was subjected to vacuum column chromatography using gradient concentration of ethyl acetate-n hexane, and obtained 11 subfractions. There were two subfractions indicated the presence of terpenoid compounds. These subfractions were grouped and subjected to antimicrobial assay. Result showed that the terpenoid compounds have antituberculosis property with minimum inhibition concentration (MIC) value of 100µg/mL. The result was supported by acid -fast Zielh-neelsen stain, which indicated that at a concentration of 100 and 200 µg/ml of the subfractions, there were no growth of Mycobacterium tuberculosis visually detected. In conclusion, Merremia mammosa is potential to be developed as antituberculosis agent. The chemical compounds responsible for this activity are currently under investigation for further study.
Cite this article:
Mangestuti Agil, Herra Studiawan, Neny Purwitasari. Efficacy of Merremia mammosa Hall Terpenoid Fraction againts Mycobacterium tuberculosis. Research Journal of Pharmacy and Technology. 2021; 14(12):6617-0. doi: 10.52711/0974-360X.2021.01143
Mangestuti Agil, Herra Studiawan, Neny Purwitasari. Efficacy of Merremia mammosa Hall Terpenoid Fraction againts Mycobacterium tuberculosis. Research Journal of Pharmacy and Technology. 2021; 14(12):6617-0. doi: 10.52711/0974-360X.2021.01143 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-12-73
1. https://www.who.int/tb/publications/global_report/tb19_Report_country_profiles_15October2019.pdf?ua=1 accessed in 21 April 2020.
2. Shruthi Ravimohan, Hardy Kornfeld, Drew Weissman, Gregory P. Bisson. 2018.Tuberculosis and lung damage: from epidemiology to pathophysiology European Respiratory Review 27 (147) 170077; DOI: 10.1183/16000617.0077-2017
3. Aslam, B., Wang, W., Arshad, M. I., Khurshid, M., Muzammil, S., Rasool, M. H., Nisar, M. A., Alvi, R. F., Aslam, M. A., Qamar, M. U., Salamat, M., & Baloch, Z. (2018). Antibiotic resistance: a rundown of a global crisis. Infection and drug resistance, 11, 1645–1658. https://doi.org/10.2147/IDR.S173867.
4. Ansori ANM, Susilo RJK, Hayaza S, et al. Renoprotection by Garcinia mangostana L. pericarp extract in streptozotocin-induced diabetic mice. Iraqi Journal of Veterinary Science. 2019; 33(1): 13-19.
5. Ansori ANM, Fadholly A, Hayaza S, et al. A review on medicinal properties of mangosteen (Garcinia mangostana L.). Research Journal of Pharmacy and Technology. 2020; 13(2): 974-982.
6. Fadholly A, Ansori ANM, Jayanti S, et al. Cytotoxic effect of Allium cepa L. extract on human colon cancer (WiDr) cells: In vitro study. Research Journal of Pharmacy and Technology. 2019; 12(7): 3483-3486.
7. Fadholly A, Ansori ANM, Proboningrat A, et al. Apoptosis of HeLa cells via caspase-3 expression induced by chitosan-based nanoparticles of Annona squamosa leaf extract: In vitro study. Indian Journal of Pharmaceutical Education and Research. 2020; 54(2): 416-21.
8. Husen SA, Wahyuningsih SPA, Ansori ANM, et al. The effect okra (Abelmoschus esculentus Moench) pods extract on malondialdehyde and cholesterol level in STZ-induced diabetic mice. Ecology, Environment and Conservation. 2019; 25(April Suppl. Issue): S50-S56.
9. Husen SA, Kalqutny SH, Ansori ANM, et al. Hepato-renal protective effects of Mangosteen (Garcinia mangostana L.) pericarp extract in streptozotocin-induced diabetic mice. Journal of Physics: Conference Series. 2020; 1445(1): 012018.
10. Hayaza S, Istiqomah S, Susilo RJK, et al. Antidiabetic activity of ketapang (Terminalia catappa L.) leaves extract in streptozotocin-induced diabetic mice. Indian Veterinary Journal. 2019; 96(12): 11-13.
11. Tacharina MR, Ansori ANM, Plumeriastuti H, et al. Beneficial effect of grinting grass (Cynodon dactylon) on the streptozotocin induced diabetes mellitus in the mice. Indian Veterinary Journal. 2020; 97(4).
12. Kurniasih RT. The Anti-Inflammatory Effects of Ethanol Extract of Bidas Upas Bulbs (Merremia mammosa Hall.f.) Topically in Swiss Mice Strain Caragen-Induced. Thesis. Yogyakarta: Faculty of Pharmacy, Sanata Dharma University; 2014.
13. Kriselina A. Test the Antibacterial Activity of Hexane and Methanol Extract from the Bidas Upas Bulbs (Merremia mammosa Hall). Thesis. Surabaya: Faculty of Pharmacy, Airlangga University; 1997.
14. Mangestuti A, Noor E, Retno W, et al. Inhibition test of Mycobacterium tuberculosis from tubers upas bidas (Merremia mammosa Hall). Final Report of the Stranas-DP2M Dikti; 2010.
15. Cantrell CL, Franzblau SG, Fischer NH. Antimycobacterial plant terpenoids.Planta Med. 2001 Nov;67(8):685-94. doi: 10.1055/s-2001-18365
16. Andreas Romulo, Ervizal A. M. Zuhud, Johana Rondevaldova & Ladislav Kokoska (2018) Screening of in vitro antimicrobial activity of plants used in traditional Indonesian medicine, Pharmaceutical Biology, 56:1, 287-293, DOI: 10.1080/13880209.2018.1462834
17. Higuchi CT, Pavan FR, Sannomiya M, et al. Triterpenes and antitubercular activity of Byrsonima crassa. Química Nova. 2008; 31: 1719-1721.
18. Gordien AY, Gray AI, Franzblau SG, et al. Antimycobacterial terpenoids from Juniperus communis L. (Cuppressaceae). Journal of Ethnopharmacology. 2009; 126(3): 500-505.