Author(s):
Mohammad Sukmanadi, Mustofa Helmi Effendi
Email(s):
mheffendi@yahoo.com
DOI:
10.5958/0974-360X.2021.00143.8 
Address:
Mohammad Sukmanadi1, Mustofa Helmi Effendi2*
1Department of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, 60115, Indonesia.
2Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, 60115, Indonesia.
*Corresponding Author
Published In:
Volume - 14,
Issue - 2,
Year - 2021
ABSTRACT:
Background: Interaction bioinformatics analysis research and prediction of protein gene panel identification as a target of evaluating the effects of damage by AFB1 that induces hepatic injury1. Capsaicin compounds are active2: 1). Activating apoptosis, 2). Inducing the cell cycle, 3). cell-regulation viability of transcription expression factors. Objective: Proving that capsaicin inhibits the expression of AKT1 and MAPK1 target proteins in mice due to the induction of AFB1 in silico and in vivo. Methods: Molecular docking (In silico) bioinformatics and hepatic lesions pathogenicity; congestion, degeneration, and necrosis (In vivo). Results: Molecular docking of Gibbs free energy is negative. Scoring synergistically inhibits the viability of the signaling pathway and prevents the occurrence of hepatic lesions. Conclusion: Capsaicin has been shown to have a protective effect against AFB-1 induction; by preventing the occurrence of hepatic lesions so that they have potential herbal medicine candidates as hepatoprotection.
Cite this article:
Mohammad Sukmanadi, Mustofa Helmi Effendi. The protective effect of Capsaicin (Capsicum annum L) against the induction of Aflatoxin B1 in hepatocytes: A study of liver Histopathology in mice (Mus musculus). Research J. Pharm. and Tech. 2021; 14(2):813-816. doi: 10.5958/0974-360X.2021.00143.8
Cite(Electronic):
Mohammad Sukmanadi, Mustofa Helmi Effendi. The protective effect of Capsaicin (Capsicum annum L) against the induction of Aflatoxin B1 in hepatocytes: A study of liver Histopathology in mice (Mus musculus). Research J. Pharm. and Tech. 2021; 14(2):813-816. doi: 10.5958/0974-360X.2021.00143.8 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-2-39
REFERENCES:
1. Shi, J.; He, J.; Lin, J.; Sun, X.; Sun, F.; Ou, C.; Jiang, C. 2016. Distinct response of the epatic transcriptome to Aflatoxin B1 induced hepatocellular carcinogenesis and resistance in rats. Sci. Rep. 6, 31898:1–31898:9.
2. Lin CH, Lu WC, Wang CW, Chan YC, Chen MK. 2013. Capsaicin induces cell cycle arrest and apoptosis in human KB cancer cells. BMC Complementary and Alternative Medicine. 13:1472- 6882.doi:10.1186/1472-6882-13-46.
3. Gürbay A, Aydın S, Girgin G, Engin AB, Şahin G. Assessment of Aflatoxin M1 Levels in Milk in Ankara, Turkey. Food Control 2006; 17 (1): 1–4.
4. Hussain, I., Anwar, J., Munawar, M. A., and Asi, M. R. 2008. Variation of Levels of Aflatoxin M1 In Raw Milk from Different Localities in The Central Areas of Punjab, Pakistan. Food Control, 19(12), 1126-1129.
5. Balogh J, Victor D III, Emad H Asham, Sherilyn Gordon Burroughs, Maha Boktour, Ashish Saharia. Xian Li. R Mark Ghobrial. Howard P Monsour Jr. 2016. Hepatocellular carcinoma: a review. J Hepatocell Carcinoma; 3: 41‐53. Published Oct 5. doi:10.2147/JHC.S61146.
6. Hastuti U.S. 2006. Pengaruh Berbagai Dosis Citrinin Terhadap Kerusakan Struktur Hepatosit Mencit (Mus Musculus) Pada Tiga Zona Lobulus Hepa Jurnal Kedokteran Brawijaya, Vol. XXII, No. 3, Desember: 121-126.
7. Susy Yunita Prabawati. 2006. Aspek Kimiawi Racun Afiatoksin Dalam Bahan Pangan Dan Pencegahannya. Kaunia Jurnal Sains Dan Teknologi Vol. II , No. 2,
8. Surh, Y.J. 2002. More Than Spice: Capsaicin in Hot Chili Peppers Makes Tumor Cells Commit Suicide. Cancer Spectrum Knowledge Environment. 94(17): 1263–1265.
9. Yu-Tsai Lin, Hung-Chen Wang, Yi-Chiang Hsu, Chung-Lung Cho, Ming-Yu Yang and Chih-Yen Chien. 2017. Capsaicin Induces Autophagy and Apoptosis in Human Nasopharyngeal Carcinoma Cells by Downregulating the PI3K/AKT/mTOR Pathway. Int. J. Mol. Sci. 18: 1343.
10. Park S.Y, Kim J.Y, Su - Mi Lee, Chung-Hwan Jun, Sung-Bum Cho, Chang - Hwan Park, Young- Eun Joo, Hyun-Soo Kim, Sung-Kyu Choi And Jong-Sun Rew. 2014. Capsaicin induces apoptosis and modulates MAPK signaling in human gastric cancer cells. Molecular Medicine Reports. 9: 499-502.
11. Kharisma, Viol Dhea, Luhur Septiadi, and Syafrudin Syafrudin. 2018. Prediction of Novel Bioactive Compound from Zingiber officinale as Non-nucleoside Reverse Transcriptase Inhibitors (NNRTIs) of HIV-1 through Computational Study. Bioinformatics and Biomedical Research Journal 1. 2: 49-55
12. Trott, Oleg, and Arthur J. Olson. 2010. Auto Dock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading. Journal of Computational Chemistry 31. 2: 455-461.
13. Shen, Haitao, et al. 2013. Aflatoxin G1-induced oxidative stress causes DNA damage and triggers apoptosis through MAPK signaling pathway in A549 cells. Food and Chemical Toxicology 62: 661-669.
14. Zhang J, Huang W, Chua SS, Wei P and Moore DD, 2002. Modulation of acetaininophen induced hepatotoxicity by xenobiotic receptor CAR. Science. 298: 422-424.
15. Cullen J M, 2005. Mechanistic classification of liver Injury. Toxicol Pathol. 33: 6-8.