Author(s):
P.R. Dwarakanath, K. Rajakumari, R. Thiruchelvi
Email(s):
rajakumari.se@velsuniv.ac.in
DOI:
10.5958/0974-360X.2021.00163.3 
Address:
P.R. Dwarakanath1, K. Rajakumari2*, R. Thiruchelvi2
1Department of Bio-Engineering, School of Engineering, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai-117, Tamil Nadu, India.
2Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai-117, Tamil Nadu, India.
*Corresponding Author
Published In:
Volume - 14,
Issue - 2,
Year - 2021
ABSTRACT:
The main objective of the project is to isolate bacteria from Forest soil and characterization of bacteria producing Glucanase. Three different bacteria are isolated from the forest soil. The Bacteria are identified using Biochemical Test. The bacterial strain identified are Bacillus, E. coli and Klebsiella. Two screening for Glucanase producing bacteria are made. The primary screening involves the modified agar diffusion with Congo red assay. The clear white zones are observed in the primary screening. The sample 3 has large white zone when compared to sample 2 and sample 1. The secondary screening involves the Glucanse assay stated by Mendal and Webber. The bacterial sample 3 produced higher concentration of Glucanase than other two bacteria. The sample 3 has produced 0.473mg of Glucose after the conversion of cellulose by the Glucanase enzyme.
Cite this article:
P.R. Dwarakanath, K. Rajakumari, R. Thiruchelvi. Isolation and Characterization of Glucanase producing bacteria from Forest Tree Litter. Research J. Pharm. and Tech. 2021; 14(2):916-920. doi: 10.5958/0974-360X.2021.00163.3
Cite(Electronic):
P.R. Dwarakanath, K. Rajakumari, R. Thiruchelvi. Isolation and Characterization of Glucanase producing bacteria from Forest Tree Litter. Research J. Pharm. and Tech. 2021; 14(2):916-920. doi: 10.5958/0974-360X.2021.00163.3 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-2-59
REFERENCES:
1. Johansson MB. Decomposition rates of Scots pine needle litter related to site properties, litter quality, and climate. Canadian Journal of Forest Research. 1994a Sep 1; 24(9):1771-81.
2. Johansson MB. Decomposition rates of Scots pine needle litter related to site properties, litter quality, and climate. Canadian Journal of Forest Research. 1994bSep 1; 24(9):1771-81.
3. Krishna MP, Mohan M. Litter decomposition in forest ecosystems: a review. Energy, Ecology and Environment. 2017 Aug 1; 2(4):236-49.
4. Wiegel J, Dykstra M. Clostridium thermocellum: adhesion and sporulation while adhered to cellulose and hemicellulose. Applied Microbiology and Biotechnology. 1984 Jul 1; 20(1):59-65.
5. Krivtsov V, Liddell K, Bezginova T, Salmond R, Staines HJ, Watling R, Garside A, Thompson JA, Griffiths BS, Brendler A. Forest litter bacteria: relationships with fungi, microfauna, and litter composition over a winter-spring period. Polish Journal of Ecology. 2005 Jan 1; 53(3):383-94.
6. Robson LM, Chambliss GH. Cellulases of bacterial origin. Enzyme and Microbial Technology. 1989 Oct 1; 11(10):626-44.
7. Kelly JM, Beauchamp JJ. Mass Loss and Nutrient Changes in Decomposing Upland Oak and Mesic Mixed-Hardwood Leaf Litter 1. Soil Science Society of America Journal. 1987; 51(6):1616-22.
8. Knapp EB, Elliott LF, Campbell GS. Microbial respiration and growth during the decomposition of wheat straw. Soil Biology and Biochemistry. 1983 Jan 1; 15(3):319-23.
9. Baldrian P, Merhautová V, Cajthaml T, Petránková M, Šnajdr J. Small-scale distribution of extracellular enzymes, fungal, and bacterial biomass in Quercus petraea forest topsoil. Biology and Fertility of Soils. 2010 Sep 1; 46(7):717-26.
10. Dilly O, Bloem J, Vos A, Munch JC. Bacterial diversity in agricultural soils during litter decomposition. Appl. Environ. Microbiol.. 2004 Jan 1; 70(1):468-74.
11. Downie B, Hilhorst HW, Bewley JD. A new assay for quantifying endo-β-D-mannanase activity using Congo Red dye. Phytochemistry. 1994 Jul 1; 36(4):829-35.
12. Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry. 1959 Mar 1; 31(3):426-8.
13. Felix CR, Ljungdahl LG. The cellulosome: the exocellular organelle of Clostridium. Annual Review of Microbiology. 1993 Oct; 47(1):791-819.
14. Amore A, Pepe O, Ventorino V, Birolo L, Giangrande C, Faraco V. Industrial waste based compost as a source of novel cellulolytic strains and enzymes. FEMS Microbiology letters. 2013 Feb 1; 339(2):93-101.
15. Adesina FC, Onilude AA. Isolation, identification and screening of xylanase and glucanase-producing microfungi from degrading wood in Nigeria. African Journal of Agricultural Research. 2013; 8(34):4414-21.