Author(s):
Novena Yety Lindawati, Dian Puspitasari, Lusia Murtisiwi, Tesia Aisyah Rahmania
Email(s):
novena_yl@stikesnas.ac.id
DOI:
10.5958/0974-360X.2021.00229.8 
Address:
Novena Yety Lindawati*, Dian Puspitasari, Lusia Murtisiwi, Tesia Aisyah Rahmania
Department of Pharmaceutical Chemistry and Pharmacology, Nasional College of Health Science (STIKES Nasional), Sukoharjo, Indonesia.
*Corresponding Author
Published In:
Volume - 14,
Issue - 3,
Year - 2021
ABSTRACT:
Diabetes mellitus (DM) is a metabolic disorder characterized by hyperglycemia associated with abnormal carbohydrate, fat, and protein metabolism caused by decreased insulin secretion or decreased insulin sensitivity, or both and causes chronic microvascular, macrovascular, and neuropathic complications. Synthetic and natural antioxidant compounds are able to control blood glucose levels and prevent complications of diabetes. Red beans (Phaseolus vulgaris L.) one type of beans have antioxidant activity. IC50 value of antioxidant activity of methanol extract of red beans was 164.44ppm. Processed red bean products in the form of tempeh fermented for 36 hours have antioxidant activity with an IC50 value of 90.84ppm. This study aims to look at the correlation of flavonoid content on antidiabetic activity in red beans (Phaseulus vulgaris L) and its processed products. In vitro antidiabetic activity testing uses the antrorone-sulfate method. Determination of total flavonoid levels using the colorimetric method with AlCl3 and CH3COOK reagents. Correlation of flavonoid content with antidiabetic activity in red beans was analyzed by Pearson correlation method from SPSS software program. The total content of flavonoids in red beans and their processing products is different. Red bean juice has a total flavonoid content of 0.21%, red bean extract has a total flavonoid content of 0.54% and the largest total flavonoid content is owned by tempe extract which is 1.39%. The value of antidiabetic activity in EC50 produced by these three samples is different where red bean juice is 458.0415ppm, red bean extract is 435.1279ppm, and red bean tempe extract is 108.0131ppm. There is a significant correlation between flavonoid content and antidiabetic activity in kidney beans (Phaseulus vulgaris L.) and its processed products.
Cite this article:
Novena Yety Lindawati, Dian Puspitasari, Lusia Murtisiwi, Tesia Aisyah Rahmania. Correlation of Flavonoid content on Antidiabetic activity in red beans (Phaseulus vulgaris L.) and its Processed Products. Research J. Pharm. and Tech. 2021; 14(3):1293-1297. doi: 10.5958/0974-360X.2021.00229.8
Cite(Electronic):
Novena Yety Lindawati, Dian Puspitasari, Lusia Murtisiwi, Tesia Aisyah Rahmania. Correlation of Flavonoid content on Antidiabetic activity in red beans (Phaseulus vulgaris L.) and its Processed Products. Research J. Pharm. and Tech. 2021; 14(3):1293-1297. doi: 10.5958/0974-360X.2021.00229.8 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-3-17
REFERENCES:
1. American Diabetes Association. Diagnosis and Classification of Diabetes Mellitus, Diabetes Care, 2010; 33(1).
2. Rupesh Gautam, S.C. Sharma. Effect of Punica granatum Linn. (Peel) on Blood Glucose Level in Normal and Alloxan- Induced Diabetic Rats. Research J. Pharm. and Tech. 5(2): Feb. 2012; Page 226-227.
3. World Health Organization (WHO), Definition, Diagnosis and classification of diabetes mellitus and its complications, Part 1: Diagnosis and classifications of diabetes mellitus, Geneva: Department of Non-communicable Disease Surveillance; 1999.
4. Shom Prakash Kushwaha, Sunil Kumar Rawat, Pavan Kumar, Abhishek, Kishu Tripathi. Coupling Antioxidant and Antidiabetic assets of 2, 4-Thiazolidinedione Derivatives. Asian J. Pharm. Ana. 1(4): Oct.-Dec. 2011; Page 71-73.
5. Preeti K. Suresh, Abhishek K. Sah, Sanjay J. Daharwal. Role of free radicals in ocular diseases: An overview. Research J. Pharm. and Tech. 7(11): Nov. 2014 Page 1330-1344.
6. Sadowska-Bartosz I and Bartosz G. Prevention of Protein Glycation by Natural Compound, Molecules, 2015; 20: 3309-3334.
7. Shom Prakash Kushwaha, Sunil Kumar Rawat, Pavan Kumar, Abhishek, Kishu Tripathi. Coupling Antioxidant and Antidiabetic assets of 2, 4-Thiazolidinedione Derivatives. Asian J. Pharm. Ana. 1(4): Oct. - Dec. 2011; Page 71-73.
8. Preeti Tiwari, Rakesh K. Patel. Estimation of Total Phenolics and Flavonoids and Antioxidant Potential of Ashwagandharishta Prepared by Traditional and Modern Methods. Asian J. Pharm. Ana. 3(4): Oct. - Dec. 2013; Page 147-152.
9. Kumar S and Pandey AK. Chemistry and Biological Activities of Flavonoids: An Overview. The Sci. World J., 2013; 16.
10. Krishna Kondragunta. V, Karuppuraj. V, Perumal. K. Antioxidant activity and Folic acid content in indigenous isolates of Ganoderma lucidum. Asian J. Pharm. Ana. 2016; 6(4): 213-215.
11. Suhaling, S., 2010, Uji Aktivitas Antioksidan Ekstrak Metanol Kacang Merah (Phaseolus vulgaris L.) dengan Metode DPPH, Skripsi, Fakultas Ilmu Kesehatan, Universitas Islam Negeri Alaudin, Makasar.
12. Racio IL, Elena P, Torinus MI, Martinez-Villaluenga C, Duenas M, Frias J. Fermentation Enhances the Content of Bioactive Compounds in Kidney Bean Extracts, Food Chem., 2015; 343-352.
13. Mahesh S. Patil, Shrikant R. Kulkarni. Studies on Ethanol Production from the Fruit Waste Using Ultrasound Assisted Fermentation. Asian J. Research Chem. 4(3): March 2011; Page 429-433.
14. Astuti M, Meliala A, Dalais FS, Wahlqvist ML. Tempe, a Nutritious and Healthy Food from Indonesia, Asia Pacific J. Clin. Nutr., 2000; 9(4): 322-325.
15. M Sugumaran, T Vetrichelvan, S Darlin Quine. Antidiabetic potential of aqueous and alcoholic leaf extracts of Pithecellobium dulce. Asian J. Research Chem. 2(1): Jan.-March, 2009; Page 83-85.
16. Mr. Israel Jeba Prabu, Mrs. Janet Anbumani. A Study to Assess the effectiveness of Video Assisted Teaching Programme on the level of knowledge regarding Blood Donation among GNM 1st year students of AMT School, Jammu. Int. J. of Advances in Nur. Management. 2020; 8(2):127-132.
17. Al-kayyis HK and Susanti H. Perbandingan Metode Somogyi-Nelson dan Antrone-Sulfat pada Penetapan Kadar Gula Pereduksi dalam Umbi Cilembu (Ipomoea batatas L.), Jurnal Farmasi Sains dan Komunitas, 2016; 13:2.
18. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of Total Flavonoid Content in Propolis by Two Complementary Colorimetric Methods, J. Food Drug Anal, 2002; 10.
19. Rini RTP. 2015, Uji aktivitas Antioksidan Kombinasi Ekstrak Etanol Kayi Secang (Caesalpinia sappon L.) dan Kacang Merah (Phaseolus vulgaris L.) dengan Menggunakan Metode DPPH (1,1-Diphenyl-2-picrylhydrazyl), Skripsi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Sebelas Maret, Surakarta.
20. Zhang Q, Lin LG, Ye WC. Techniques for Extraction and Isolation of Natural Products: a Comprehensive Review, Chin. Med, 2018; 13:20
21. Eirene GF, Matheis FJDP, Tanasale, Jolantje L, Dominggus M, Regy T. Phytochemical Screening of Water Extract of Gayam (Inocarpus edulis) Bark and Its Amylase Inhibitor Activity Assay, IOP Conf Ser: Mater Sci Eng, 2019; 509 012074.
22. Marliana SD, Suryanti V, Suyono. Skrining Fitokimia dan Analisis Kromatografi Lapis Tipis Komponen Kimia Buah Labu Siam (Sechium edule Jacq. Swartz) dalam Ekstrak Etanol, Jurnal Biofarmasi, 2005; 3(1): 26-31.
23. Malgorzata W, Joanna H, Konrad PM. Effect of Solid-State Fermentation with Rhizopus oligosporus on Bioactive Compound and Antioxidant Capacity of Raw and Roasted Buckwheat Groats. Ital. J. Food Sci, 2015; 27.
24. Soares LAL, Bassani VL, Ortega GG, Petrovick PR. Total Flavonoid Determination for The Quality Control of Aqueous Extractive from Phyllanthus Niruri L, Lat. Am. J. Pharm, 2003; 22(3): 203-7.