ABSTRACT:
The biological active compounds that are present in plants referred as phytochemicals. These phytochemicals derived from different parts of plants such as leaves, barks, seed, seed coat, flowers, roots and pulps and thereby used as sources of direct medicinal agents. Phytochemistry describes the large number of secondary metabolic compounds present in the plants. The plants are the reservoirs of naturally occurring chemical compounds and of structurally diverse bioactive molecules. Chromatographic techniques have significant role in natural products chemistry as well as contribute dramatically in the discovery of novel and innovative compounds of pharmaceutical and biomedical importance. The study absorbed on visual demonstration of fractionation and isolation of Lantana camara leaves of secondary metabolites using column-chromatographic techniques. Isolation of bioactive compounds using column-chromatographic involves: a) Preparation of sample; b) Packing of column; c) Pouring of sample into the column; d) Elution of fractions e) Analysis of each fractions using thin layer chromatography. However, depending on nature of research, compounds can be further purified using Gas chromatography and Mass spectrometry (GC-MS), infrared spectroscopy (IR) and high performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR) spectral analyses.