Author(s):
Ira Humairah, Lina Lukitasari, Retno Handajani, Mochamad Amin, Citrawati Dyah Kencono Wungu, Edhi Rianto, Arif Nur Muhammad Ansori
Email(s):
humairah.ira@gmail.com
DOI:
10.52711/0974-360X.2021.00354 
Address:
Ira Humairah1*, Lina Lukitasari1, Retno Handajani1,2, Mochamad Amin1,2, Citrawati Dyah Kencono Wungu1, Edhi Rianto1, Arif Nur Muhammad Ansori3,4
1Department of Physiology and Medical Biochemistry, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
2Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
3Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
4Professor, Nidom Foundation, Surabaya, Indonesia.
*Corresponding Author
Published In:
Volume - 14,
Issue - 4,
Year - 2021
ABSTRACT:
Every year, there is an increase in the number Intravenous Drug Users (IDUs) in Indonesia, in which they have a greater risk of getting Hepatitis C Virus (HCV) infection. This study was aimed to determine HCV prevalence and genotypes among IDUs in Surabaya. A total 25 IDUs were tested for anti-HCV. Reverse-transcription PCR in the NS5B region and 5'UTR was carried out on serum with positive anti-HCV, continued with sequencing and HCV genotype/subtype determination. The prevalence of HCV infection in IDUs in Surabaya based on positive anti-HCV was 96%(24/25). Sequencing analysis showed that 92%(22/24) could be determined their genotypes and subtypes. 40.9% (9/22) samples had genotype 1 with subtype 1a 31.82%(7/22) and subtype 1c 9.09%(2/22). 40.9%(9/22) samples had genotype 3, with subtype 3a 18.18%(4/22) and 3k 22.73%(5/22). 13.64%(4/22) samples had genotype 4 with subtype 4a, and 4.55% (1/22) sample had genotype 6 subtype 6n. The prevalence of HCV infection was high among IDUs in Surabaya. In this study, genotypes 6 was found in which it is rarely found in Indonesia, so there is a possibility that there are different HCV genotype patterns in each region of Indonesia.
Cite this article:
Ira Humairah, Lina Lukitasari, Retno Handajani, Mochamad Amin, Citrawati Dyah Kencono Wungu, Edhi Rianto, Arif Nur Muhammad Ansori. Prevalence and Genotype Distribution of Hepatitis C Virus among Intravenous Drug users (IDUs) in Surabaya, Indonesia. Research Journal of Pharmacy and Technology. 2021; 14(4):1999-3. doi: 10.52711/0974-360X.2021.00354
Cite(Electronic):
Ira Humairah, Lina Lukitasari, Retno Handajani, Mochamad Amin, Citrawati Dyah Kencono Wungu, Edhi Rianto, Arif Nur Muhammad Ansori. Prevalence and Genotype Distribution of Hepatitis C Virus among Intravenous Drug users (IDUs) in Surabaya, Indonesia. Research Journal of Pharmacy and Technology. 2021; 14(4):1999-3. doi: 10.52711/0974-360X.2021.00354 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-4-30
REFERENCES:
1. Espírito-Santo MP, Carneiro MAS, Reis NRS, et al. Genotyping hepatitis C virus from hemodialysis patients in Central Brazil by line probe assay and sequence analysis. Braz J Med Biol Res. 2007; 40(4): 545-550.
2. WHO. Hepatitis C. World Health Organization. Available from: http://www.who.int/mediacentre/factsheets/fs164/en/; 2018.
3. Man John Law L, Landi A, Magee WC, et al. Progress towards a hepatitis C virus vaccine. Emerg Microbes Infect. 2013; 2(11): e79.
4. Badan Penelitian dan Pengembangan Kesehatan. Riset Kesehatan Dasar (RISKESDAS) 2013. Lap Nas 2013. 2013; 1-384.
5. Amin M, Juniastuti J, Utsumi T, et al. The prevalence and subtype distribution of hepatitis c virus infection among hemodialysis patients in a private hospital in Surabaya, Indonesia. Microbiol Indones. 2013; 6(4): 173-179.
6. Murphy EL, Bryzman SM, Glynn SA, et al. Risk factors for hepatitis C virus infection in United States blood donors. NHLBI Retrovirus Epidemiology Donor Study (REDS). Hepatology. 2000; 31(3): 756-762.
7. Tatiana M, Narciso-Schiavon L, Schiavon L. Epidemiology of hepatitis C virus infection. Rev Assoc Med Bras. 57(1): 105-110.
8. Messina JP, Humphreys I, Flaxman A, et al. Global distribution and prevalence of hepatitis C virus genotypes. Hepatology. 2015; 61(1): 77-87.
9. Soler M, Pellerin M, Malnou CE, et al. Quasispecies heterogeneity and constraints on the evolution of the 5' noncoding region of hepatitis C virus (HCV): relationship with HCV resistance to interferon-alpha therapy. Virology. 2002; 298(1): 160-173.
10. Murphy DG, Willems B, Deschênes M, et al. Use of sequence analysis of the NS5B region for routine genotyping of hepatitis C virus with reference to C/E1 and 5′ untranslated region sequences. J Clin Microbiol. 2007; 45(4): 1102-1112.
11. Apichartpiyakul C, Chittivudikarn C, Miyajima H, et al. Analysis of hepatitis C virus isolates among healthy blood donors and drug addicts in Chiang Mai, Thailand. J Clin Microbiol. 1994; 32(9): 2276-2279.
12. Doi H, Apichartpiyakul C, Ohba KI, et al. Hepatitis C virus (HCV) subtype prevalence in Chiang Mai, Thailand, and identification of novel subtypes of HCV major type 6. J Clin Microbiol. 1996; 34(3): 596-574.
13. McCabe SE, Morales M, Cranford JA, et al. Race/ethnicity and gender differences in drug use and abuse among college students. J Ethn Subst Abuse. 2008; 6(2): 75-95.
14. Baden R, Rockstroh JK, Buti M. Natural history and management of hepatitis C: Does sex play a role? J Infect Dis. 2014; 209 Suppl 3: S81-5.
15. Kong F, Pan Y, Chi X, et al. Factors associated with spontaneous clearance of hepatitis C virus in Chinese population. Biomed Res Int. 2014; 2014: 527030.
16. Mulyanto. Viral hepatitis in Indonesia: Past, present, and future. Euroasian J Hepatogastroenterol. 2016; 6(1): 65-69.
17. Backmund M, Reimer J, Meyer K, et al. Hepatitis C virus infection and injection drug users: Prevention, risk factors, and treatment. Clin Infect Dis. 2005; 40(s5): S330-S335.
18. Lim SG, Aghemo A, Chen PJ, et al. Management of hepatitis C virus infection in the Asia-Pacific region: An update. Lancet Gastroenterol Hepatol. 2017; 2(1): 52-62.
19. Kurniawan H. Analisis genotipe virus hepatitis C (VHC) pada pasien dengan infeksi VHC kronik. Indones J Heal Sci. 2013; 4(1): 15-20.
20. Perhimpunan Peneliti Hati Indonesia. The 10th Liver Update & the Annual Scientific Meeting of INA ASL/PPHI 2017. Jakarta; 2017.
21. Lusida MI, Nagano-Fujii M, Nidom CA, et al. Correlation between mutations in the interferon sensitivity-determining region of NS5A protein and viral load of hepatitis C virus subtypes 1b, 1c, and 2a. J Clin Microbiol. 2001; 39(11): 3858-3864.
22. Utama A, Tania NP, Dhenni R, et al. Genotype diversity of hepatitis C virus (HCV) in HCV-associated liver disease patients in Indonesia. Liver Int. 2010; 30(8): 1152-1160.
23. Pawlotsky JM, Aghemo A, Back D, et al. EASL recommendations on treatment of hepatitis C 2015. J Hepatol. 2015; 63(1): 199-236.