ABSTRACT:
Sphaeranthus indicus is one of the extremely precious herbs in the Indigenous System of Medicine. The present study was carried out to acute, subacute and LD50 values of methanolic extract of S. indicus leaves in Swiss mice of both sexes. The acute toxicity studies were conducted oral administration of 1.75, 5.5, 17.5, 55, 175, 550, 2000mg/kg body weight SIME used. Observations were recorded systemically up to 24 h after dose administration for behavior related to nervous system response or autonomic functions. Food and water intake, body weight variations, hematological and biochemical parameters were assessed. In sub acute toxicity treatment there were no significant variation in the body weights and haematological parameters except dose-dependent increase in lymphocyte count was noted in both sexes supported immunostimulant activity. Pathologically, significant protective effect on hepatic, renal functions and decreased cholesterol, triglyceride levels. The results did not show any treatment related abnormalities in terms of hematological and biochemical parameters in sub-acute toxicity. After acute administration, no mortality was recorded in mice treated with the SIME orally at a dose of 1000mg/kg. The LD50 values were determined using graphical method; we found a broad therapeutic window and a high therapeutic index value showed that the LD50 of the extract is 2480mg/kg. The results suggest that the plant seems to be high margin of drug safety in mice.
Cite this article:
Gajendra Pratap Choudhary, Ashutosh Pal Jain. Evaluation of Acute, Subacute and LD50 values of Methanolic extract of Sphaeranthus indicus leaves in Albino mice. Research Journal of Pharmacy and Technology. 2021; 14(5):2487-2. doi: 10.52711/0974-360X.2021.00438
Cite(Electronic):
Gajendra Pratap Choudhary, Ashutosh Pal Jain. Evaluation of Acute, Subacute and LD50 values of Methanolic extract of Sphaeranthus indicus leaves in Albino mice. Research Journal of Pharmacy and Technology. 2021; 14(5):2487-2. doi: 10.52711/0974-360X.2021.00438 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-5-22
REFERENCES:
1. Palmer ME, et al. Adverse events associated with dietary supplements: an observational study. The Lancet. 2003; 361(9352): 101-106.
2. Gogate VM. Ayurvedic pharmacology and therapeutic uses of medicinal plants (Dravyaganvigyan). Mumbai: Bhartiya Vidya Bhavan. 2000: 112-114.
3. Kirtikar KR, Basu BD. Indian medicinal plants. International Book Distributors, Dehradun.1987: 343.
4. Nadkarni KM. Indian Material Medica, III Edn, Vol I. Popular Prakashan Pvt. Ltd. Bombay. 1976: 1162.
5. Mathew JE., et al. Mast cell stabilizing effects of Sphaeranthus indicus. Journal of ethnopharmacology. 2009; 122(2): 394-396.
6. Naqvi BS, et al. Antibacterial activity in fruits and vegetables. Pakistan journal of Pharmacology. 1998; 15:7–11.
7. Shekhani MS, et al. An immunostimulant sesquiterpene glycoside from Spharanthus indicus. Phytochemistry. 1990; 29(6): 2573.
8. Heinrich M, et al. Ethnopharmacology of Mexican Asteraceae (Compositae). Annual Review of Pharmacology and Toxicology. 1998; 38: 539–565.
9. Jain A, Basal E. Inhibition of Propionibacterium acnes-induced mediators of inflammation by Indian herbs. Phytomedicine. 2003; 10: 34–38.
10. Ambavade SD, et al. Pharmacological evaluation of the extracts of Sphaeranthus indicus flowers on anxiolytic activity in mice. Indian Journal of Pharmacology. 2006; 38: 254–259.
11. Mhetre NA, Ambavade SD, Bodhankar SL. Neuroleptic activity of extract of Sphaeranthus indicus in mice. Indian Journal of Natural Products. 2006; 22: 24–27.
12. Shirwaikar A, Prabhu KS, Punitha IS. In vitro antioxidant studies of Sphaeranthus indicus (Linn) Indian Journal of Experimental Biology. 2006; 44: 993–996.
13. Jha RK, Garud N, Nema RK. Excision and incision wound healing activity of flower head alcoholic extract of Sphaeranthus indicus Linn. in albino rats. Global Journal Pharmacology. 2009; 3: 32–37.
14. Nanda BK, et al. Analgesic and Antipyretic activity of whole parts of Sphaeranthus indicus Linn. Journal of Chemical and Pharmaceutical Research. 2009; 1: 207–212.
15. Roux, Sylvain, Evelyne Sablé, and Roger D. Porsolt. "Primary observation (Irwin) test in rodents for assessing acute toxicity of a test agent and its effects on behavior and physiological function." Current Protocols in Pharmacology.2004, 27(1):10.
16. Ecobichon, DJ. The Basis of Toxicology Testing. CRC Press, New York. 1997: 43–86.
17. Veerappan, A, et al. Acute and subacute toxicity studies of Aegle marmelos Corr., an Indian medicinal plant. Phytomedicine. 2007; 14(3): 209-215.
18. Vaghasiya YK, Shukla VJ, Chanda SV. Acute oral toxicity study of Pluchea arguta boiss extract in mice. J Pharmacol Toxicol. 2011; 6(2): 113-23.
19. Bothiraja C, et al. Acute and subacute toxicity study of andrographolide bioactive in rodents: Evidence for the medicinal use as an alternative medicine. Comparative Clinical Pathology. 2013; 22(6): 1123-1128.
20. Adeneye AA, Olagunju JA. Preliminary hypoglycemic and hypolipidemic activities of the aqueous seed extract of Carica papaya Linn in Wistar rats. Biology and Medicine. 2009; 1(1): 1-10.
21. Witthawaskul P, et al. Acute and subacute toxicities of the saponin mixture isolated from Schefflera leucantha Viguier. Journal of Ethnopharmacology. 2003; 89(1): 115-121.
22. El Hilaly, et al. Acute and chronic toxicological studies of Ajuga iva in experimental animals. Journal of Ethnopharmacology. 2004; 91(1): 43-50.
23. Gowda S, et al. Markers of renal function tests. North American Journal of Medical Sciences. 2010; 2(4): 170.