Author(s):
Anand Babu K, Ram Narayanan R
Email(s):
anandbabu23@rediffmail.com
DOI:
10.52711/0974-360X.2021.00535 
Address:
Anand Babu K1*, Ram Narayanan R2
1Department of Pharmaceutical Chemistry, College of Pharmacy, Kannur Medical College, Anjarakandy, Kannur -670612, Kerala.
2Department of Pharmaceutics, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai - 116, Tamil Nadu.
*Corresponding Author
Published In:
Volume - 14,
Issue - 6,
Year - 2021
ABSTRACT:
Objective: The research is about the stability of ophthalmic products as they start to produce irritancy effects during their shelf life. The aim of the present study is to assess the stability of certain ophthalmic products by the HET-CAM (Hen’s Egg Test-Chorioallatonic membrane) Method. Method: Incubated Eggs were utilized for performing the HET-CAM assay according to the protocol prescribed by ICCVAM (The Inter agency Coordinating Committee on the Validation of Alternative Methods). Results: Calculation of various irritancy factors like hemorrhage time, vascular lysis time and coagulation time and to calculate HET-CAM score. Statistical interpretation of the data obtained by one-way ANOVA. Conclusion: Results have shown that the few ophthalmic products are slight and moderate irritants. None of the products are strong irritants. However further studies to be conducted to confirm the potential and safety of the products.
Cite this article:
Anand Babu K, Ram Narayanan R. Determination of Ocular Irritancy Potential of Ophthalmic products using HET-CAM Method. Research Journal of Pharmacy and Technology. 2021; 14(6):3063-6. doi: 10.52711/0974-360X.2021.00535
Cite(Electronic):
Anand Babu K, Ram Narayanan R. Determination of Ocular Irritancy Potential of Ophthalmic products using HET-CAM Method. Research Journal of Pharmacy and Technology. 2021; 14(6):3063-6. doi: 10.52711/0974-360X.2021.00535 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-6-25
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