Author(s):
Arvind P, Priyadarshini S, Duraiswamy B, Dhanabal SP, Ramu G
Email(s):
ramupharmu@jssuni.edu.in
DOI:
10.52711/0974-360X.2021.00540 
Address:
Arvind P1, Priyadarshini S, Duraiswamy B, Dhanabal SP, Ramu G*
Dept of Pharmacognosy, JSS College of Pharmacy, JSS Academy of Higher Education & Research, Ooty, Nilgiris, Tamilnadu, India.
*Corresponding Author
Published In:
Volume - 14,
Issue - 6,
Year - 2021
ABSTRACT:
Objective: In the aim of refining mucilage polysaccharide extracted from the leaf of Basella alba, extraction and differential precipitation of the protein content was studied. This was attempted by pH adjustment by adding Trichloroacetic acid (TCA) and Hydrochloric acid (HCl). Materials and Methods: The presence of residual protein before and after deproteinization in the polysaccharide solution was detected by UV spectrum analysis. The % polysaccharide, % polysaccharide loss, protein concentration and the deproteinization efficiency were studied as comparative indices to evaluate the precipitation experimental conditions using pH adjustment. Results: The result showed that 10% w/v TCA precipitated over 80% of the protein when the pH of the aqueous polysaccharide solution was 3. Discussion: TCA was proved to be superior to hydrochloric acid as evidenced by the highest deproteinization efficiency (83.3%). The polysaccharides of all the extracted solutions obtained were identified with only slight variations in percentage. The Hcl method excelled over the TCA method in obtaining polysaccharides with little lower percentage of polysaccharide loss (13.94 %). Conclusion: The TCA method will offer a room for deproteinization of polysaccharides if optimization is studied.
Cite this article:
Arvind P, Priyadarshini S, Duraiswamy B, Dhanabal SP, Ramu G. UV Detection and Avoidance of Protein in Basella alba leaf Mucilage Polysaccharide by differential precipitation. Research Journal of Pharmacy and Technology. 2021; 14(6):3093-6. doi: 10.52711/0974-360X.2021.00540
Cite(Electronic):
Arvind P, Priyadarshini S, Duraiswamy B, Dhanabal SP, Ramu G. UV Detection and Avoidance of Protein in Basella alba leaf Mucilage Polysaccharide by differential precipitation. Research Journal of Pharmacy and Technology. 2021; 14(6):3093-6. doi: 10.52711/0974-360X.2021.00540 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-6-30
REFERENCES:
1. Qadry JS, Shah, Qadry. Pharmacognosy. Ahmedabad, India: B S Shah Prakashan; 2008.
2. Evans WC, Trease and Evan’s. Pharmacognosy, 14th Edn, WB Saunders Company: London; 210, 2001
3. Beri RG and Rollings JE. A technique for the rapid removal of proteins from cell extracts containing polysaccharides. Biotechnol. Tech 1995; 9:783-86.
4. Ramu G, Krishna Mohan G, Jayaveera K.N. Preliminary investigation of patchaippasali mucilage (Basella alba) as tablet binder. Int J Green Pharm 2011;5(1):24-27.
5. Fang G, Hammar S, Grumet R. A quick and inexpensive method for removing polysaccharides from plant genomic DNA. Bio. Tech 1992;13(1):52-54.
6. Bradford MM. Analytical Biochemistry 1976; 72:248-54.
7. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. 1956. Colorimetric method for determination of sugars and related substances. Anal Chem 29 : 350 - 356.
8. Arifkhodzhaev AO. Polysaccharides of saponin-bearing plants. An investigation of the carbohydrates of acanthophyllum pungens. Chem. Natl Comp 1997; 5:693-95.
9. Huang GL, Chen Y, Wang XQ. Extraction and deproteinization of pumpkin polysaccharide. Int J Food Sci Nutr 2011b;62(6):568-71.
10. Huang GL, Quan Shu SG, Tiantian Cai, Yunqing Liu, Feng Xiao. Preparation and deproteinization of garlic polysaccharide. Int J Food Sci Nutr 2012;63(6):739-41.
11. Yi Chen, Mingyong Xie, Wenjuan Li, Hui Zhang, Shaoping Nie, Yuanxing Wang, Chang Li. A effective method for deproteinization of bioactive polysaccharides extracted from Lingzhi (Ganoderma atrum). Food Sci & Biotech 2012;21(1)191-98.
12. Ahmad Fazilat. Production, isolation, purification and partial characterization of an extracellular acid protease from Aspergillus niger. Int J of Adv Res in Biological Sciences 2016;3(3):32-38
13. Staub AM. 1965. Removal of proteins : Sevage method. Methods Carbohydr Chem 5:5-6.