Author(s):
Bhong Prabha N., Naikawade Nilofar. S., Mali Pratibha. R., Bindu Madhavi. S.
Email(s):
prabha.1may@gmail.com
DOI:
10.52711/0974-360X.2021.00580 
Address:
Bhong Prabha N.1, Dr. Naikawade Nilofar. S.2, Mali Pratibha. R.3, Bindu Madhavi. S.4
1Assistant Professor, Marathwada Mitramandals College of Pharmacy, Kalewadi, Pune, Maharashtra 411033.
2H.O.D., Appasaheb Birnale College of Pharmacy, Sangli.
3NET JRF, Department of Biochemistry, Shivaji University, Kolhapur.
4Assistant Professor, Marathwada Mitramandals College of Pharmacy, Kalewadi, Pune.
*Corresponding Author
Published In:
Volume - 14,
Issue - 6,
Year - 2021
ABSTRACT:
Objectives: The present study designed to evaluate the Antiasthmatic activity of aqueous extract of bark of Eugenia Jambolana (AEEJ) on in vitro and in vivo animal models. Materials and methods: Different in vitro and in vivo animal models was used to study the anti asthmatic activity as isolated goat tracheal chain preparation, Acetylcholine and Histamine induced bronconstriction in guinea pigs, effect of drug extract on histamine release from mast cell was checked by clonidine-induced mast cell degranulation, and milk-induced eosinophilia and leukocytosis. Results: In-vitro study on goat tracheal chain preparation revealed that aqueous extract of Eugenia jambolana (AEEJ)bark exerted antagonistic effect on the histamine induced contraction. (P<0.05) The guinea pigs when exposed to 0.2% histamine aerosol showed signs of progressive dyspnoea leading to convulsions. AEEJ significantly prolonged the latent period of convulsions (PCT) as compared to control following the exposure of histamine (0.2%) aerosol (P<0.01). The observation of present study indicates aqueous extract of Eugenia jambolana shows significant inhibition of milk induced eosinophilia and leukocytosis. Group of animals pretreated with aqueous Eugenia jambolana bark extract showed significant reduction in degranulation of mast cells when challenged with clonidine. The prevention of degranulation process by the aqueous Eugenia jambolana bark extract (P<0.01) indicates a possible stabilizing effect on the mast cells, indicating mast cell stabilizing activity. Conclusions: Thus, AEEJ showed antihistaminic, mast cell stabilizing and protective in guinea pigs against histamine induced PCD, reduced eosinophilia and leukocytosis and hence possesses potential role in the treatment of asthma.
Cite this article:
Bhong Prabha N., Naikawade Nilofar. S., Mali Pratibha. R., Bindu Madhavi. S. In-vitro and in-vivo Evaluation of Anti-asthmatic Activity of Eugenia jambolana bark. Research Journal of Pharmacy and Technology. 2021; 14(6):3337-2. doi: 10.52711/0974-360X.2021.00580
Cite(Electronic):
Bhong Prabha N., Naikawade Nilofar. S., Mali Pratibha. R., Bindu Madhavi. S. In-vitro and in-vivo Evaluation of Anti-asthmatic Activity of Eugenia jambolana bark. Research Journal of Pharmacy and Technology. 2021; 14(6):3337-2. doi: 10.52711/0974-360X.2021.00580 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-6-70
REFERENCES:
1. Behera D, Sehgal S. Bronchial asthma - Issues for the developing world. Indian Journal of Medical Research. 2015; 141: 380-382.
2. Samir S, Kintu P and Kerai S, Evaluation of anti-asthmatic activity of Centratherum anthelminticumlinn. in experimental animals, Journal of Pharmaceutical Research 2017; 16. (1): 19- 24
3. Gupta, KB., Verma, M. Nutrition and Asthma. Lung India 2007; 24(3) p. 105-114
4. Kelly HW, Sorkness CA. Athma. In Pharmacotherapy A Pathological Approach Dipirio Edited by JT, Yee GC., McGRAW Hill, Medical Publishing Division, 1999, 5th edition: p.475-510
5. Kirtikar, KR. Basu, BD. 1991. In Indian Medicinal Plants. Vol. II (Periodical Experts, New Delhi), p. 1052-53.
6. OECD guideline for testing of chemicals; Acute Oral Toxicity- Up-and-Down Procedure:425 adapted 17th dec 2001;1-26
7. Bhujbal, SS, Kumar D. Antiasthmatic Activity of Roots of Hemidesmus indicus R. Br. Pharmacologyonline 2009, (1) p. 209-216.
8. Tripathi RM., Das PK. 1977 Studies on Antiasthmatic and antianaphylactic activity of Albizzia lebbeck. Indian Journal of Pharmacy. 1977; 9(3) : 189-194.
9. Vogel WH., Scholkens, BA, Sandow J, Muller G, Drug Discovery and Evaluation, Pharmacological Assays, 2ed edition Springer p.362.
10. Natarajan P and Sakthiraj K, pharmacological screening of anti-asthmatic activity of Anisomelesmalabarica (l).r.br. exsims, Int. Res. J. Pharm. 2019, 10 (4) 179-183
11. Krishnaraj S, Aathirai K, Saravana Balaji MD and Premlal KR, In-vitro and In-vivo anti-asthmatic activity of ethanolic leaf extract of Caesalpiniapulcherrima, International Journal of Phytopharmacology. 10(1), 2019, 1-5.
12. Urvisha V. Bangoriya, H. M. Tank, Patel DM, Tan PH, Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis in clonidine induced mast cell degradation in Rats, J Pharm Sci Bioscientific Res. 2016 6(1):120-123
13. Dnyaneshwar J Taura, Ravindra Y Patil, Different screening methods for asthma: A Review, Journal of Pharmacy Research 2010, 3(12), 2828-2830
14. Aslam RP, Gautam V, M Sabu, Curcuma caesia rhizomes: evaluation of antiasthmatic effect by using clonidine induced mast cell degranulation Neuropharmac J; Sept-Dec 2016; 1 (1): 7-12
15. Vadnere, G. P., Rahul S. S., et. Al, 2007 Studies on Antiasthmatic Activity of Aqueous Extract of C. phlomidis. Pharmacologyonline1: p. 487-494.
16. S. Rajasekaran, evaluation of effects of Parthenium hysterophorus l., leaves extracts against milk-induced eosinophilia in mice, IJPSR, 2014; Vol. 5(4): 1303-1306.
17. Sheetal Singh, G. S Chakaraborthy, Evaluation of antiasthmatic and antioxidant potentials of seeds of extract of Strychnus nuxvomica L. Research Journal of Pharmacy and Technology, 2020;13(1)