Author(s): Saumya S, Shalini S, S. Leelavathy, P. Deepa Sankar

Email(s): pdeepasankar@vit.ac.in

DOI: 10.52711/0974-360X.2021.00655   

Address: Saumya S1, Shalini S1, S. Leelavathy1, P. Deepa Sankar2*
1School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore - 632 014, Tamil Nadu, India.
2VIT School of Agricultural Innovations and Advanced Learning (VAIAL), Vellore Institute of Technology, Vellore - 632 014, Tamil Nadu, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 7,     Year - 2021


ABSTRACT:
Objective: The study focuses on formulating and validating low-cost media for in vitro callus induction and development in Catharanthus roseus. Methods: Percentage response, nature and mass of in vitro grown C. roseus callus was recorded for the conventional MS media with casein hydrolysate (1g/L) and three low-cost media supplemented with casein hydrolysate (1g/L). The effect of two hormonal combinations, viz., 1.5mg/L 2,4-D + 1mg/L Kin and 1mg/L BAP + 1mg/L NAA had been studied for callus development. Results: Two media combinations including MS (urea replacing NH4NO3) + table sugar (30g/L) + casein hydrolysate (1 g/L) + Food grade agar (FGA, 7g/L) and MS + table sugar (30g/L) + casein hydrolysate (1g/L) + sago (80g/L) was observed to take less number of days to callus induction, 11 and 12 days respectively, than the conventional MS media + casein hydrolysate (1g/L) + gelrite (2.2g/L) with 2,4-D (1.5mg/L) + Kin (1mg/L), which took 13 days to callus induction. MS (urea replacing NH4NO3) + table sugar (30g/L) + casein hydrolysate (1g/L) + FGA (7g/L) with 2,4-D (1.5mg/L) + Kin (1mg/L) gave rise to high callus mass, averaging to 12.351g, at the end of 4th subculture and also, high percentage of cost reduction per liter of media was observed compared to conventional MS media with casein hydrolysate. Conclusion: The present study indicated MS (urea replacing NH4NO3) + casein hydrolysate (1g/L) + FGA (7g/L) with 2,4-D (1.5mg/L) + Kin (1mg/L) as a better media for developing and proliferating C. roseus callus In vitro.


Cite this article:
Saumya S, Shalini S, S. Leelavathy, P. Deepa Sankar. Low-Cost Media for In-vitro Callus Development in Catharanthus roseus (L) G. Don. Research Journal of Pharmacy and Technology. 2021; 14(7):3785-8. doi: 10.52711/0974-360X.2021.00655

Cite(Electronic):
Saumya S, Shalini S, S. Leelavathy, P. Deepa Sankar. Low-Cost Media for In-vitro Callus Development in Catharanthus roseus (L) G. Don. Research Journal of Pharmacy and Technology. 2021; 14(7):3785-8. doi: 10.52711/0974-360X.2021.00655   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-7-52


REFERENCES:
1.    Kiruba, S., Mahesh, M., Nisha, S.R., Paul, Z.M. and Jeeva, S., 2011. Phytochemical analysis of the flower extracts of Rhododendron arboreum Sm. ssp. nilagiricum (Zenker) Tagg. Asian Pacific Journal of Tropical Biomedicine, 1(2), pp. S284-S286.
2.    Raja, R.D.A., Jeeva, S., Prakash, J.W., Antonisamy, J.M. and Irudayaraj, V., 2011. Antibacterial activity of selected ethnomedicinal plants from South India. Asian Pacific Journal of Tropical Medicine, 4(5), pp.375-378.
3.    Jaleel, C.A., Gopi, R., Manivannan, P., Sankar, B., Kishorekumar, A. and Panneerselvam, R., 2007. Antioxidant potentials and ajmalicine accumulation in Catharanthus roseus after treatment with giberellic acid. Colloids and surfaces B: Biointerfaces, 60(2), pp.195-200.
4.    Tiong, S., Looi, C., Hazni, H., Arya, A., Paydar, M., Wong, W., Cheah, S.C., Mustafa, M. and Awang, K., 2013. Antidiabetic and antioxidant properties of alkaloids from Catharanthus roseus (L.) G. Don. Molecules, 18(8), pp.9770-9784.
5.    Bakrudeen, A., Subha Shanthi, G., Gouthaman, T., Kavitha, M. and Rao, M., 2011. In vitro micropropagation of Catharanthus roseus—an anticancer medicinal plant. Acta Botanica Hungarica, 53(1-2), pp.197-209.
6.    Mehta, J., Upadhyay, D., Paras, P., Ansari, R., Rathore, S. and Tiwari, S., 2013. Multiple shoots regeneration of (anticancer plant) Catharanthus roseus–an important medicinal plant. Am J Pharm Tech Res, 3, pp.785-793.
7.    Ibrahim, M., Mehjabeen, S.S. and Narsu, M.L., 2011. Pharmacological evaluation of Catharanthus roseus. International Journal of Pharmaceutical Applications, 2(3), pp.165-173.
8.    Rajora, R.K., Sharma, N.K. and Sharma, V., 2013. Effect of plant growth regulators on micropropagation of Catharanthus roseus. International Journal of Advanced Biotechnology and Research, 4(1), pp.123-130.
9.    Iwase, A., Aoyagi, H., Ohme-Takagi, M. and Tanaka, H., 2005. Development of a novel system for producing ajmalicine and serpentine using direct culture of leaves in Catharanthus roseus intact plant. Journal of bioscience and bioengineering, 99(3), pp.208-215.
10.    Pietrosiuk, A., Furmanowa, M. and Łata, B., 2007. Catharanthus roseus: micropropagation and in vitro techniques. Phytochemistry reviews, 6(2-3), pp.459-473.
11.    Shukla, A.K., Shasany, A.K., Gupta, M.M. and Khanuja, S.P., 2006. Transcriptome analysis in Catharanthus roseus leaves and roots for comparative terpenoid indole alkaloid profiles. Journal of experimental botany, 57(14), pp.3921-3932.
12.    Murashige, T. and Skoog, F., 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologiaplantarum, 15(3), pp.473-497.
13.    Veerabathini, S, Sarang, S, Shalini, S and Deepa Sankar, P. (2015). Standardization of friable callus development in Catharanthus roseus (Linn.) G. Don. International Journal of Pharmacy and Pharmaceutical Sciences. 7. 111-113.
14.    Haq, R., Naz, S., Aslam, F. and Manzoor, F., 2013. Comparison of in vitro response of micropropagation and callogenesis of medicinal plant, Vinca rosea. J Agric Res, 51(1), pp.9-17.
15.    Taha, H.S., El-Bahr, M.K. and Seif-El-Nasr, M.M., 2008. In vitro studies on Egyptian Catharanthus roseus (L.) G. Don.: 1-Calli production, direct shootlets regeneration and alkaloids determination. J. Appl. Sci. Res, 4(8), pp.1017-1022.
16.    Thorpe, T.A., 2007. History of plant tissue culture. Molecular biotechnology, 37(2), pp.169-180.
17.    Sharifi, A., Moshtaghi, N. and Bagheri, A., 2010. Agar alternatives for micropropagation of African violet (Saintpaulia ionantha). African Journal of Biotechnology, 9(54), pp.9199-9203.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

1.3
2021CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank

Journal Policies & Information


Recent Articles




Tags


Not Available