Author(s):
Sneha S, Mrunal Palsokar, Vemula Sai Jahnavi, Anwesha Sarkar, K. V. Bhaskara Rao
Email(s):
kvbhaskararao@vit.ac.in
DOI:
10.52711/0974-360X.2021.00735 
Address:
Sneha S, Mrunal Palsokar, Vemula Sai Jahnavi, Anwesha Sarkar, K. V. Bhaskara Rao*
Marine Biotechnology Laboratory, Department of Biomedical Sciences, School of Biosciences and Technology, Vellore Institute of Technology, Vellore - 632014, Tamil Nadu, India.
*Corresponding Author
Published In:
Volume - 14,
Issue - 8,
Year - 2021
ABSTRACT:
Protease constitutes the major group of catalytic enzymes which is involved in hydrolyzing peptide bond of proteins. Marine sediment sample were collected and protease producing bacterial isolates were identified by using casein as a substrate. The organisms were characterized by biochemical test and identified as Bacillus sp. In order to check for the production of protease enzyme, quantitative protease assay and Lowry’s method of protein estimation was carried out. The crude extract of protease was subjected for blood stain removal activity and the enzyme proved to be efficient which removed the stain in 15 min. The purpose of the current study is to isolate, identify, characterize and to carry out applications of protease enzyme from marine bacteria isolated from mangrove sediment samples.
Cite this article:
Sneha S, Mrunal Palsokar, Vemula Sai Jahnavi, Anwesha Sarkar, K. V. Bhaskara Rao. Isolation, Characterization and Application of Protease Enzyme from Marine Bacteria. Research Journal of Pharmacy and Technology. 2021; 14(8):4236-0. doi: 10.52711/0974-360X.2021.00735
Cite(Electronic):
Sneha S, Mrunal Palsokar, Vemula Sai Jahnavi, Anwesha Sarkar, K. V. Bhaskara Rao. Isolation, Characterization and Application of Protease Enzyme from Marine Bacteria. Research Journal of Pharmacy and Technology. 2021; 14(8):4236-0. doi: 10.52711/0974-360X.2021.00735 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-8-41
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