Khan N.I., Hatapakki B.C., Tamboli A.M.
Khan N.I.1*, Hatapakki B.C.2, Tamboli A.M.1
1Department of Pharmacology, Sahyadri College of Pharmacy, Methwade, Maharashtra, India – 413307.
2Department of Pharmacognosy, P.S.P.S.'s Indira Institute of Pharmacy, Sadavali (Devrukh), Maharashtra, India – 415804.
Volume - 14,
Issue - 9,
Year - 2021
Since there are many bioactive compounds present in plant material containing various multi-component mixtures, their separation and determination is important to identify the active phytoconstituents responsible for pharmacological activity. Practically most of them have to be separated by column chromatographic techniques. The present study deals with the identification and characterization of bioactive principles from the stems of Mimosa hamata. The isolated fractions from the ethanolic extracts of stems of Mimosa hamata was carried out by column chromatography. For separation of a bioactive compound, the solvent system tried for column chromatography was Chloroform: Methanol in various ratios like 90:10, 80:20, 70:30, and 60:40 amongst these we could separate the first fraction at 80:20 and second fraction at 70:30. Two flavonoids compounds were isolated from the ethanolic extracts of stems of the medicinal plant Mimosa hamata (Willd). Based on chemical and spectral analyses their structures were elucidated as Quercetin and Cirsimaritin. From the above study it reveals the presence of flavonoids in the ethanolic extracts of stems of Mimosa hamata (Willd.) was isolated using column chromatography further subjected to characterization of isolated compounds including UV, IR, NMR and Mass spectroscopic study for elucidating the structure of the two separated compounds. The interpreted data concluded that, both isolated compounds are flavonoids i.e. Cirsimaritin and Quercetin. The pharmacological effect of Mimosa hamata stems may be due to the presence of its phytoconstituents flavonoids.
Cite this article:
Khan N.I., Hatapakki B.C., Tamboli A.M. Isolation and Characterization of Flavonoids of the Ethanolic extracts of stems of Mimosa hamata (Willd.) by Chromatographic techniques. Research Journal of Pharmacy and Technology. 2021; 14(9):4602-8. doi: 10.52711/0974-360X.2021.00800
Khan N.I., Hatapakki B.C., Tamboli A.M. Isolation and Characterization of Flavonoids of the Ethanolic extracts of stems of Mimosa hamata (Willd.) by Chromatographic techniques. Research Journal of Pharmacy and Technology. 2021; 14(9):4602-8. doi: 10.52711/0974-360X.2021.00800 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-9-14
1. Jitendra Patel, Venkateshwar Reddy, G.S. Kumar. Evaluation of hepatoprotective activity of ethanolic extract of Diospyros melanoxylon (Roxb) leaves against CCl4 induced hepatotoxicity in albino rats. Research J. Pharm. and Tech. 8(5): May, 2015; Page 571-574.
2. Subin Mary Zachariah, N. Aleykutty, Vidya Viswanad, Halima O.A. An Overview on Hepatoprotective Activity of Natural Products. Research J. Pharm. and Tech. 5(3): Mar.2012; Page 317-321.
3. Renu Singh, Jasrai YT. Mimosa hamata (Willd.), a plant with anti-pathogenic properties. International Journal of Medicinal and Aromatic Plants. 2012; 2(4): 677-682.
4. Barneby RC. Sensitive Censitae: A description of the genus Mimosa Linnaeus (Mimosaceae) in the new world. In Memoirs of the New York Botanical Garden. 1992; 65.
5. Hussain N, Modan MH, Shabbir SG, Antimicrobial principles in Mimosa hamata. J. Nat. Prod.1979; 42: 525-527.
6. Nadkarni A.K, Nadkarni KM., Indian Materia Medica. Popular Book Depot, Bombay 1954.
7. Chopra RN, Nayar SL, Chopra IC. Glossary of Indian Medicinal Plants. CSIR, New Delhi 1956.
8. Mukadam DS, Balkhande LD, Umalkar GV. Antifungal activities in deproteinized leaf extract of weeds and non-weeds. Indian J. Microbiol. 1976; 16: 78-79.
9. Jain R, Arora R, Alam S, Jain SC. Pharmacological evaluation of Mimosa hamata roots. Fitoterapia. 1997; 68: 377-378.
10. Jain SC, Jain R, Singh R. Antioxidant activity of mimonosides isolated from in vitro regenerated plants of Mimosa hamata (Willd.). Indian J. Plant Physiol. 2009; 14: 124-129
11. Parvej S, Kumkum Mathur, Goyal M, Yadav SK. Mimosa hamata Willd: A Review on Ethnobotanical, Phytochemical and Pharmacological Profile. International Journal of Pharmaceutical and Biological Archives. 2016; 8 (1): 10-12.
12. Wagner, G., Blatt, S. Plant drug analysis, a thin layer chromatography, Atlas, 2nd Edition, CS Pringer Verlag. 1996. 352-355
13. Jamal AK, Yaacob WA, Laily BD, A Chemical Study on Phyllanthus reticulatus. Jour of Physical Sci. 2008; 19 (2): 45–50.
14. AbdulmalikI A, Sule MI, Musa AM, Yaro AH, Abdullahi MI, Abdulkadir MF, Yusuf H. Isolation of Steroids from Acetone Extract of Ficus iteophylla. British Jour of Pharmacol and Toxicology. 2011; 2(5): 270-272.
15. Hassan AAD. Studies on the constituents of Ficus capensis Thunb. Pak Jour of Soc Sci, 2005; 3(5): 751-54.
16. Mahadeva Rao U. S., C. Shanmuga Sundaram, J. Sivakumar. Isolation and Characterization of Phytochemical Constituents and its Antibacterial Activity of Brassica oleracea var acephala. Research J. Pharm. and Tech 2019; 12(1): 297-302.
17. Adithya Chandrashekar, Anuraag Muralidharan, Ananthamurthy Koteshwara, Angel Treasa Alex, V. M. Subrahmanyam. Isolation and Characterization of an actinomycete strain producing an antifungal metabolite effective against Candida albicans. Research J. Pharm. and Tech. 2019; 12(10): 4601-4606.
18. Sachin K Jain, Gajendra Pratap Choudhary, Dinesh Kumar Jain. Isolation and Characterization of two New Flavonoids Derived from Leaves of Jatropha gossypifolia Linn. Research J. Pharm. and Tech. 2017; 10(2): 426-430.
19. Mahadeva Rao U. S., C. Shanmuga Sundaram, J. Sivakumar. Isolation and Characterization of Phytochemical Constituents and its Antibacterial Activity of Brassica oleracea var acephala. Research J. Pharm. and Tech 2019; 12(1): 297-302.
20. Bhurat M.R, Kawatikwar P. S., Sanghavi R.S. Isolation and Characterization of Eulophia herbacea Rhizomes Mucilage. Research J. Pharm. and Tech. 4(10): Oct. 2011; Page 1615-1617.
21. O.S.S. Chandana, D. Swapna, R. Ravichandra Babu. HPLC determination of Sildenafil Tartrate and its related Substances along with some Supportive Studies using MS, XRD and NMR. Research J. Pharm. and Tech 2018; 11(5): 2086-2093.
22. Shruthi Bharadwaj, Sheeja. L, Lakshmi. D, Sajidha Parveen. K. 1H NMR Analysis and Bio-autography Screening of Methanol Extract of Sargassum wightii by Chromatographic Separation. Research J. Pharm. and Tech. 2017; 10(2): 473-479.
23. Anket Sharma, Vinod Kumar, Parminder Kaur, Ashwani Kumar Thukral, Renu Bhardwaj. Phytochemical and Elemental Analysis of Brassica juncea L. Leaves using GC-MS and SEM-EDX. Research J. Pharm. and Tech. 2015; 8(12): 1662-1664.