In-situ gel forming formulations are a novel concept of providing drugs to individuals as a liquid dosage form yet achieving sustained release of the drug for the required duration. Different polymer based distribution schemes have been created that can boost the residence time of the product at the drug absorption location. These formulations exist as a flowing system before administration and at the physiological environment it gets converted into viscoelastic gel. The available therapies are to minimize the bacterial infection and to regenerate the damage done by infection and inflammation. The therapies involve systemic therapy, conventional therapy, as well as local therapy. Aim of the present work was to formulate in-situ gel of ofloxacin and it is evaluated for antibacterial activity and stability and it showed good antibacterial activity for both gram positive and gram negative microorganisms and was stable at refrigerated temperature over three months. The results indicated that the formulation could be utilized to maintain the localized drug concentration for a longer period and increase patient compliance with lesser adverse effects.
Cite this article:
R. R. Baghwan, A. W. Ambekar, S. S. Tamboli. Formulation, Development and Evaluation of in-situ Periodontal Gel Containing Ofloxacin. Research Journal of Pharmacy and Technology. 2021; 14(9):4609-4. doi: 10.52711/0974-360X.2021.00801
R. R. Baghwan, A. W. Ambekar, S. S. Tamboli. Formulation, Development and Evaluation of in-situ Periodontal Gel Containing Ofloxacin. Research Journal of Pharmacy and Technology. 2021; 14(9):4609-4. doi: 10.52711/0974-360X.2021.00801 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2021-14-9-15
1. Nitin Gaikwad, Abhishek Pujari, Indrajeet Mane, Ganesh Vambhurkar, Pravin Honmane. Formulation and Evaluation of Hair gel containing Unani medicine. Asian Journal of Pharmaceutical Analysis. 2018; 8: 129-136.
2. Saudagar R. B., Sarika V. Khandbahale. In-situ Nasal Gel – A Review. Asian Journal of Research in Pharmaceutical Sciences. 2017; 7: 23-32.
3. Bajpai Vibha. In-situ gel nasal drug delivery system-A Review. International Journal of Pharmaceutical Sciences. 2014; 4: 577-80.
4. Aikawa K. Drug release from pH-response Polyvinylacetal diethyl aminoacetate hydrogel and application to nasal delivery. International Journal of Pharmacy. 1998; 168: 181-8.
5. Akshay Yadav, Shrinivas Mohite, Chandrakant Magdum. Preparation and Evaluation of Antibacterial Herbal Mouthwash against Oral Pathogens. 2020; 10: 149-152.
6. Bhardwaj TR, Kanwar M. Natural gums and modified natural gums as sustained release carriers. Drug Development and Industrial Pharmacy. 2000; 26: 1025-38.
7. Himanshu Bhusan Samal, Itishree Jogamaya Das, Niranjan Patta, P. N. Murthy. Design and Development of Dental Film Containing Aloe Vera for the treatment of Human Periodontal Diseases. Asian Journal of Pharmacy and Technology. 2015; 5: 273-280.
8. Chand P, Kothiyal P. In situ gel; Review. Indian Journal of Pharmaceutical and Biological Research. 2016; 4(2)11-19.
9. Chenite A, Chaput C. Novel injectable solution of chitosan from biodegradable gels in situ. Biomaterials. 2000; 21: 2155-61.
10. Inayathulla, Prakash Goudanavar, Ankit Acharya. Development and Evaluation of in-situ gel containing Linezolid and Diclofenac Sodium in the treatment of Periodontitis. Asian Journal of Pharmacy and Technology. 2020; 10: 20-26
11. Kant A, Reddy S. In situ gelling system- an overview. Pharmacologyonline 2011; 2: 28-44.
12. Kashyap N. Design and evaluation of biodegradable, biosensitive in situ gelling systems for pulsatile delivery of insulin. Biomaterials. 2007; 28: 2051-60.
13. Kumar S, Himmelstein K. Modification of in situ gel behaviour of carbopol solutions by hydroxypropylmethylcellulose. Journal of Pharmaceutical Sciences. 1995; 84:344-8.
14. Miyazaki S. In situ gelling gellan formulations as vehicles for oral drug delivery. Journal of Controlled Release. 1999; 60: 287-95.
15. Miyazaki S. In situ gelling xyloglucan formulations for sustained release ocular delivery of Pilocarpine hydrochloride. International Journal of Pharmacy. 2001; 229: 29-36.
16. Miyazaki S. Thermally reversible xyloglucan gels as vehicles fpr rectal drug delivery. Journal of Controlled Release. 1998; 56: 75-83.
17. Miyazaki S, Kawasaki N. Comparison of in situ gelling formulations for the oral delivery of cimetidine. International Journal of Pharmacy. 2001; 220: 161-8.
18. Motto F. In vitro assessment of new embolic liquids prepared from preformed polymers and water miscible solvents aneurysm treatment. Biomaterials. 2000; 21: 803-11.
19. Nirmal HB. In situ gel: new trends in sustained and controlled drug delivery system. International Journal of Pharmaceutical Technology and Research. 2010; 2(2): 1398-1408.
20. Cackrabarty S, Nath B. A review on oral in situ gel for periodontities. World Journal of Pharmaceutical Research. 2018; 7(11): 266-270.
21. Dr. Janhavi Manohar, Dr. Asha. Anti-protease activity of Lavender on Chronic Periodontitis patients – An ex-vivo study. Asian Journal of Pharmaceutical Research. 2020; 10: 95-100.
22. Dumitriu S. Hydrogels based on polysaccharides. In: Dumitriu S. Polysaccharides in medical applications. Marcel Dekker Inc, New York. 1996; 125-242.
23. Rawat S, Warade S. In situ gel formulation of ornidazole for the treatment of periodontal disease. Current Pharmaceutical Research. 2010; 1(1): 60-69.
24. Shaurya M, Shivamurthy R, Mohammed Gulzar A. Moxifloxacin in situ gel as an adjunct clinico-microbiological study. International Journal of Oral Health Sciences. 2018; 8(1): 19-25.
25. Harish NM, Prabhu P, Charyulu RN, Gulzar MA. Formulation and evaluation of in situ gels containing clotrimazole for oral candidiasis. International Journal of Pharmaceutical Sciences. 2009; 71(4): 421-427.
26. Gorle A, Yadav R. Formulation and evaluation of in situ gel containing ciprofloxacin hydrochloride in the treatment of periodontitis. Asian Journal of Pharmaceutical and Clinical Research. 2017; 10(6): 154-59.
27. Pujitha CH. Formulation and characterization of ofloxacin ophthalmic gel. Indian Journal of Pharmacy and Pharmacology. 2017; 4(2): 105-109.
28. Chandana Pasupuleti, Abdul Saleem Mohammed, Nuha Rasheed, Kathula Umadevi, Duggi Adilakshmi. Simultaneous Formulation, Estimation and Evaluation of Ofloxacin Microbeads. Asian Journal of Pharmaceutical Analysis. 2016; 6: 167-182.
29. Maha MA. Formulation in vitro characterization and clinical evaluation of curcumin in situ gel for treatment of periodontitis. Drug Delivery. 2017; 24(1): 133-42.
32. Ranch K. Ph.D. Thesis 2012 Pharm Technology, Chapter 2 Introduction, Shodhganga, 30.
33. Rakde A. In-situ gelling characteristics of Gellan gum at various simulated independent physiological conditions. Journal of Chemical and Pharmaceutical Research. 2015; 7(2): 819-29.
34. Hareesh KB, Mohammed GA, Narayan CR. Development and evaluation of in situ gels of moxifloxacin for the treatment of periodontitis. Indonesia Journal of Pharmacy. 2012; 23(3): 141-6.
35. Audumbar Mali, Santosh Jadhav, Rahul Gorad, Aamer Quazi, Ritesh Bathe, Manojkumar Patil, Ashpak Tamboli. Simultaneous UV Spectrophotometric Methods for Estimation of Cefixime Trihydrate and Ofloxacin in Bulk and Tablet Dosage form. Asian Journal of Pharmaceutical Research. 2016; 6: 100-106.
36. Chandana Pasupuleti, Abdul Saleem Mohammed, Nuha Rasheed, Kathula Umadevi, Duggi Adilakshmi. Simultaneous Formulation, Estimation and Evaluation of Ofloxacin Microbeads. Asian Journal of Pharmaceutical Analysis. 2016; 6: 167-182.
37. Priyanka Dhalkar, Shivrani Jagtap, Suraj Jadhav Mayuresh Redkar, Biradev Karande. Formulation and Evaluation of in-situ Gel Model Naproxen. Asian Journal of Pharmacy and Technology. 2019; 9: 204-207.
38. Rahana P, Dr. Sujit S. Nair. Formulation and evaluation of in-situ gel for the treatment of periodontal disease. World Journal of Pharmacy and Pharmaceutical Sciences. 2018; 7: 1308-17.
39. Dhabi MR, Nagori S. Formulation development of smart gel periodontal drug delivery system for local delivery of chemotherapeutic agents with application of experimental design. Drug Delivery. 2010; 17(7): 520-31.
40. Pandit NK, Kisaka J. Loss of gelation ability of pluronic F127 in the presence of some salts. International Journal of Pharmacy. 1996; 145: 129-36.
41. Borole P. Preparation and evaluation of in situ gel of levofloxacin hemihydrate of treatment of periodontal disease. International Journal of Pharma and Bio Sciences. 2013; 2(3): 185-96.
42. Bhimani DR, Patel JK, Patel VP, Detroja CM. Development and evaluation of floating in situ gelling system of clarithromycin. International Journal of Pharmaceutical Research and Development. 2011; 3: 32-40.
43. Himanshu G, Aarti S. Ion activated bioadhesive in situ gel of clindamycin for vaginal application. Int J Drug Deliv. 2009; 1: 32-40.
44. Ariyana A, David S, Irman E, Dan HB. Formulation and in vitro evaluation of alginate based metronidazole periodontal gel. Asian Journal of Pharmaceutical and Clinical Research. 2014; 7(1): 223-7.