Author(s): Noor Salma, Chandra Prakash K, Syed Sagheer Ahmed, Saba Tabassum, Ahalya Devi K H


DOI: 10.52711/0974-360X.2021.00822   

Address: Noor Salma1*, Chandra Prakash K2, Syed Sagheer Ahmed3, Saba Tabassum4, Ahalya Devi K H5
1Department of Pharmacology, D R Karigowda College of Pharmacy, Hassan, Karnataka, India.
2,3Department of Pharmacology, Sri Adichunchanagiri College of Pharmacy, Adichunchanagiri University, BG Nagara, Karnataka, India.
4Government Unani Hospital, Nagamangala, Karnataka, India.
5Department of Pharmacology, Bharathi College of Pharmacy, K.M Doddi, Karnataka, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 9,     Year - 2021

Diuresis can be beneficial in removing toxins from the body. Globally traditional and folklore medicines plays a vital role in health care services. Plectranthus amboinicus belongs to Lamiaceae family is an Indian medicinal plant which has been utilized traditionally to treat various diseases. However, there is no systematic methodology for the use of Plectranthus amboinicus stem as a diuretic. Hence the present study is proposed to evaluate the diuretic activity of various solvent extract of Plectranthus amboinicus stem in the rat model. The acute toxicity study was performed in albino mice by fixed-dose method (OECD guidelines number 425) of CPCSEA. The diuretic activity was carried out using the Lipschitz method. The total urine volume collected in 24hours was observed. Sodium, potassium and chloride concentration in urine were also recorded. The ionic concentrations were recorded calorimetrically. Acute toxicity study proved no toxicity and mortality in any of the animal up to the dose of 2000mg/kg bodyweight. All three extracts of Plectranthus amboinicus have exhibited dose-dependent diuretic activity by increasing urine volume and excreting sodium ions in urine. The urine pH was also elevated. From the study, it can be concluded that the plant Plectranthus amboinicus could be considered as a diuretic agent. This current study encourages the use of this plant in folk medicine. Further study is required for isolation, characterization and formulation of the active constituents responsible for its diuretic activity.

Cite this article:
Noor Salma, Chandra Prakash K, Syed Sagheer Ahmed, Saba Tabassum, Ahalya Devi K H. Comparative Study of different Solvent Extract of Plectranthus amboinicus Stem for their Diuretic Activity. Research Journal of Pharmacy and Technology. 2021; 14(9):4727-0. doi: 10.52711/0974-360X.2021.00822

Noor Salma, Chandra Prakash K, Syed Sagheer Ahmed, Saba Tabassum, Ahalya Devi K H. Comparative Study of different Solvent Extract of Plectranthus amboinicus Stem for their Diuretic Activity. Research Journal of Pharmacy and Technology. 2021; 14(9):4727-0. doi: 10.52711/0974-360X.2021.00822   Available on:

1.    Agunu A, Abdurahman EM, Andrew GO, Muhammed Z. Diuretic activity of the stem-bark extracts of Steganotaenia araliacea hochst. Journal of Ethnopharmacology. 2005; 96 (3): 471-475.
2.    Sirisha N, Sreenivasulu M, Sangeeta K, Latha GS, Devi AL, Chetty CM. A review on herbal diuretics. Research journal of pharmacy and technology.2011; 4(3): 335-348.
3.    WHO (2008) Traditional Medicine, Fact sheet.
4.    Ogbe RJ, Yahaya AA, Anefu E. Aqueous ethanolic extract of Mangifera indica stem bark effect on the biochemical and haematological parameters of albino rats. Archives of Applied Science Research. 2012; (4): 1618-1622.
5.    Sabarinath C, Sudhakar P, Shanmuganath C. Phytochemical and antibacterial screening on leaves of Solanum torvum. Asian journal of research in pharmaceutical sciences.2018; 8(3): 2231-5659
6.    Sawant R, Baghkar A, Jagtap S, Harad L, Chavan A, Khan NA, Yevale RP, Kale MK. A review on-Herbs in anticancer. Asian Journal of Research in Pharmaceutical Science. 2018; 8(4): 179-84.
7.    Prashar D, Saklani S. Pharmaceutical and economical aspects of medicinal herbs: an overview. Research Journal of Pharmacognosy and Phytochemistry. 2011; 3(5): 187-190.
8.    Kirtikar KR and Basu BD. Indian Medicinal Plants. Lalit Mohan Basu, Allahabad. 1935.
9.    Brain KR and Turner TD. The practical evaluation of phytopharmaceuticals. Wright Science Technica, Bristol. 1975; pp. 4-9.
10.    Halliwell B. How to characterize an antioxidant: an update. Biochemical Society Symposia, Portland Press Limited. 1995; vol 61: pp. 73-101.
11.    Squadriato GI, Pelor WA. Free radical oxidative chemistry of nitric oxide: The roles of superoxide, peroxynitrite, and carbon dioxide. Biology and Medicine. 1998; 25: 392-403.
12.    Manjamalai A, Narala Y, Haridas A, Grace VB. Antifungal, anti-inflammatory and GCMS analysis of methanolic extract of Plectranthus amboinicus leaf. International Journal of Current Pharmaceutical Research. 2011; 3(2): 129-36.
13.    Tiwari DK, Nagar HE, Dwivedi GA, Tripathi RK, Jena JI. Evaluation of anti-anxiety activity of Plectranthus amboinicus (Lour.) on rats. Asian Journal of Pharmaceutical and Clinical Research. 2012; 5(4): 110-3.
14.    Yuthistran RA, Balakrishnan CA, Buddhan RB. Antibiotic effect of leaf extract from Plectranthus amboinicus (lour) spreng in asthma. International Journal of Advance Research and Innovation. 2015; 2 (3): 430-432.
15.    Santhiya M, Margret MS, Jansi M. Antimicrobial analysis, phytochemical assessment and determination of compounds in the medicinal plant Plectranthus amboinicus. International Journal of Research in Plant Science. 2013; 3(2): 14-17.
16.    Shenoy S, Kamathan P, Azhar M, Prabhu K, Hegde S, Amberkar MB. Hepatoprotective activity of ethanolic extract of Plectranthus Amboinicus against antitubercular drugs induced hepatotoxicity in wistar rats. International Journal of Innovative Pharmaceutical Sciences and Research. 2014; 2(6): 1027-33.
17.    Ramalakshmi P, Subramanian N, Saravanan R, Mohanakrishnan H, Muthu M. Anticancer effect of Coleus ambionicus (Karpooravalli) on human lung cancer cell line (a549). International Journal of Development Research. 2014; 11 (4): 2442-2449.
18.    Raghavi S, Karthigeyan VG, Prabhu S. Protective effect of Plectranthus amboinicus leaf extract containing luteolin flavonoid in isoproterenol hydrochloride induced myocardial infarction in Rats-an assessment on biochemical and cellular changes. Research Journal of Engineering and Technology. 2013; 4(4): 195-198.
19.    Kumar P, Kumar N. Pharmacognostical, Pharmacological studies and traditional uses of Plectranthus amboinicus: a review. Research Journal of Pharmacognosy and Phytochemistry.2019; 11(4): 244-250
20.    Kiran BV, Biswal B, Reddy GN, Kanthal LK, Sridhar N. Evaluation of anthelmintic activity of Eichhornia crassipes roots. Research Journal of Pharmacology and Pharmacodynamics. 2013; 5(3): 183-184.
21.    Khandelwal. Practical Pharmacognosy. Nirali Publications, Pune. 1995; 1st ed: pp. 140-143.
22.    Kokate CK, Purohit AP and Gokhle SB. Practical Pharmacognosy. Nirali Prakashan, Pune. 2005; 4th ed: pp. 8-11.
23.    Momin MA, Rashid M, Urmi KF, Rana S. Phytochemical screening and investigation of antioxidant and cytotoxicity potential of different extracts of selected medicinal plants of Bangladesh. Research Journal of Pharmacy and Technology. 2013; 6(9): 1042-1050.
24.    Lipschitz WL, Hadidian Z, Krepscar S. Bioassay of diuretics. Journal of Pharmacology and Experimental Therapeutics. 1943: 79-97.
25.    Nedi T, Mekonnen N, Urga K. Diuretic effect of the crude extracts of Carissa edulis in rats. Journal of Ethnopharmacology. 2004; 95(1): 57-61.
26.    World Health Organization and International Society of Hypertension Writing Group. World Health Organization (WHO) and International Society of Hypertension (ISH) statement on management of hypertension. Journal of Hypertension. 2003; 21(11): 1983-1992.
27.    Howland RD, Myek MJ, Harvey RA and Champe PC. Lippincott's Illustrated Reviews. Pharmacology. Baltimore Lippincott Williams and Wilkins. 2006; 3rd ed: pp. 257-69.
28.    Chodera A, Dabrowska A, Sloderbach L. Effect of flavonoid fractions of Solidago virgaurea L on diuresis and levels of electrolytes. Acta Poloniae Pharmaceutica. 1991; 48: 35-37.
29.    Rizvi SH, Shoeb A, Kapil RS, Popli SP. Two diuretic triterpenoids from Antidesma menasu. Phytochemistry. 1980; 19(11): 2409-10.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

56th percentile
Powered by  Scopus

SCImago Journal & Country Rank

Recent Articles


Not Available