Author(s): Furqan Mohammed Al-Asady, Dalia Abdulzahra Al-Saray


DOI: 10.52711/0974-360X.2021.00851   

Address: Dr. Furqan Mohammed Al-Asady1, Dr. Dalia Abdulzahra Al-Saray2
1College of Pharmacy, University of Al-Ameed, Karbala Po Box 198, Iraq.
2College of Dentistry, Babylon University, Department of Basic Sciences, Iraq.
*Corresponding Author

Published In:   Volume - 14,      Issue - 9,     Year - 2021

Objective: Evaluate the impacts of rifampicin on certain sperm function parameters and to determine whether rifampicin has an impact on chromatin quality or sperm DNA integrity. Materials and Methods: Forty two male adult rats were subjected to this study. The entire rats were subjected to random division into six groups; four rifampicin- treated groups and two control groups. Rifampicin- treated groups were treated with a dose of either (27mg/kg/day) or (54mg/kg/day) and for each treatment dose, the treatment persists for either 14 days or 28 days. Certain parameters of sperm function including sperm concentration and sperm motility were assessed. Furthermore, analysis of sperm DNA integrity and chromatin quality were also studied. Results: No significant changes related to sperm concentration were observed when rifampicin was given in different doses and different durations. A significant change in sperm motility were recorded only when rifampicin was given in high dose for 28 days and there was a significant reduction in sperm progressive and total motility. Rifampicin showed a significant increase in sperm DNA staining capability when the dose and duration was increased. Administration of rifampicin in high dosage for 28 days represented in larger adverse impact on structure of sperm chromatin. Conclusion: Rifampicin could negatively affect male fertility potential in rats mainly through affecting the quality of sperm chromatin structure.

Cite this article:
Furqan Mohammed Al-Asady, Dalia Abdulzahra Al-Saray. Impacts administration of Rifampicin on sperm DNA integrity and Male Reproductive System parameters in rats. Research Journal of Pharmacy and Technology. 2021; 14(9):4897-2. doi: 10.52711/0974-360X.2021.00851

Furqan Mohammed Al-Asady, Dalia Abdulzahra Al-Saray. Impacts administration of Rifampicin on sperm DNA integrity and Male Reproductive System parameters in rats. Research Journal of Pharmacy and Technology. 2021; 14(9):4897-2. doi: 10.52711/0974-360X.2021.00851   Available on:

1.    Natali A, Turek P. An assessment of new sperm tests for male infertility. Urology. 2011; 77(5): 1027-1034.‏
2.    Soleimani L, Lapidus K, Iosifescu D. Diagnosis and treatment of major depressive disorder. Neurologic Clinics. 2011; 29(1): 177-193.
3.    Adikwu E, Brambaifa N. Toxicological Effect of Perfloxacin on Testicular Function of Male Guinea Pigs. Asian journal of Experimental Biological Sciences. 2012; 3(1): 28-33.
4.    Laurence L, Randa H and Bjorn C. Goodman and Gilman's the Pharmacological Basis of Therapeutics, 13th Edition. 2018.‏
5.    Desai D. A review: validated analytical methods developed on antitubercular drug, rifampicin. Journal of Pharmaceutical Science and Bioscience Research. 2015; 5: 254-265.
6.    Gandigawad P, Hogade A, Patil P, Malur P. Effect of rifampicin, isoniazid on acute and subacute inflammation in male Wistar rats: an experimental study. International Journal of Basic and Clinical Pharmacy. 2017; 4(4): 682-685.
7.    Huang J, Zhang C, Zhang D, Li L, Chen X, Xu D. Rifampicin-Induced Hepatic Lipid Accumulation: Association with Up-Regulation of Peroxisome Proliferator-Activated Receptor γ in Mouse Liver. PloS one. 2016; 11(11): e0165787.
8.    Raj RR, Pratibha SW, Amol BC, Ozhukil KR, Rajendra PG, Saurabh P. Effect of rifampicin and erythromycin on the initiation of galactose induced cataract in rats. Journal of Pharmacology and Pharmacotherapeutics. 2012; 3(4): 330-332.
9.    Nitin M, Syeda S, Amreen B, Ansari F. Protective effect of aqueous extract of Sacharum officinarum leaves against Rifampicin-induced Hepatotoxicity in Rats. Research Journal of Pharmacy and Technology. 2013; 6(8): 885-888.
10.    World Health Organization. WHO laboratory manual for the examination and processing of human semen.5th ed. Geneva: World Health Organization 2010.
11.    AL-Dujaily S, AL-Janabi A, Nori M. Effect of Glycyrrhiza extract on in vitro sperm activation of asthenospermic patients. J of Babyl Univer 2006; 11(3): 477- 483.
12.    Lu J, Huang Y, Lü N. WHO Laboratory Manual for the Examination and Processing of Human Semen: its applicability to andrology laboratories in China. Nat J of Androl 2010;16(10):867-871.‏
13.    Agarwal A, Said T. Sperm chromatin assessment in Textbook of Assisted Reproductive Techniques,4thed, Volume (1): Laboratory Perspectives 2012; p(75).
14.    Talebi A, Vahidi S, Aflatoonian A, Ghasemi N, Ghasemzadeh J, Firoozabadi R, Moein M, et al. Cytochemical evaluation of sperm chromatin and DNA integrity in couples with unexplained recurrent spontaneous abortions. Andrologia 2012; 44(s1): 462-470.‏
15.    Bryman A, Cramer D. Quantitative Data Analysis with IBM SPSS 17, 18 and 19: A Guide for Social Scientists. New York: Routledge 2011.
16.    Awodele O, Akintonwa A, Osunkalu V, Coker H. Modulatory activity of antioxidants against the toxicity of rifampicin in vivo. Revista do Instituto de Medicina Tropical de Sao Paulo 2010; 52(1): 43-46.‏
17.    Zobeiri F, Salami S, Sadrkhanlou R, Peirouvi T. Role of mitochondria in ciprofloxacin-induced apoptosis in murine sperm cells. Reproduct Sci 2013b;20(9):1090-1095.
18.    Abd-Allah A, Aly H, Moustafa A, Abdel-Aziz A, Hamada F. Adverse testicular effects of some quinolone members in rats. Pharm Res 2000; 41: 211-219.
19.    Sanocka D, Kurpisz M. Reactive oxygen species and sperm cells. Reproduct Biol and Endocrin 200; 2(1): 12-26.
20.    Correia J, Michelangeli F, Publicover S. Regulation and roles of Ca2+ stores in human sperm. Reproduct 2015; 150(2): R65-R76.
21.    Shayakhmetova G, Bondarenko L, Kovalenko V. Damage of testicular cell macromolecules and reproductive capacity of male rats following co-administration of ethambutol, rifampicin, isoniazid and pyrazinamide. Interdisciplinary Toxicol 2012; 5(1): 9-14.‏
22.    Alifano P, Palumbo C, Pasanisi D, Talà A. Rifampicin-resistance, rpob B polymorphism and RNA polymerase genetic engineering. J of Biotechnol 2015; 202: 60-77.‏

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

56th percentile
Powered by  Scopus

SCImago Journal & Country Rank

Recent Articles


Not Available