Author(s): Herman Y. L. Wihastyoko, Setyawati Soeharto, Edi Widjajanto, Kusworini, Bambang Pardjianto


DOI: 10.52711/0974-360X.2021.00862   

Address: Herman Y. L. Wihastyoko1,2, Setyawati Soeharto3, Edi Widjajanto4, Kusworini4, Bambang Pardjianto2,5
1Doctoral Program, Faculty of Medicine, Brawijaya University, Malang 65145, Indonesia.
2Plastic, Aesthetic and Reconstruction Consultant, Saiful Anwar General Hospital, Malang 65111, Indonesia.
3Pharmacology Department, Faculty of Medicine, Brawijaya University, Malang 65145, Indonesia.
4Clinical Pathology Department, Faculty of Medicine, Brawijaya University, Malang 65145, Indonesia.
5Faculty of Medicine and Health Sciences, State Islamic University of Malang, Malang 65144, Indonesia.
*Corresponding Author

Published In:   Volume - 14,      Issue - 9,     Year - 2021

Aims: This study aims to identify the potential of papain as a candidate for the treatment modality for abnormal scars via in silico studies. Methods: We determined the potential mechanism of the process of collagen degradation by papain by investigating its cleavage site-specificity and identifying human papain-like enzymes that have comparable biological activity in degrading collagen in the extracellular matrix using Merops, Bioedit, String DB and Cytoscape software. Results: Papain targets QQ_D (Glutamine-Glutamine Aspartic acid) motif for degradation while collagen only has QQ (Glutamine-Glutamine) motif. Additionally, the homology result showed that Cathepsin B has a closer relationship with papain compared with another candidate, Cathepsin K. Conclusion: Papain is a potential therapeutical modality candidate in degrading collagen in abnormal scars with an indirect mechanism as indicated by its cleavage site-specificity and its relationship with Cathepsin B, which degrades collagen via ubiquitin (UBC) proteasome.

Cite this article:
Herman Y. L. Wihastyoko, Setyawati Soeharto, Edi Widjajanto, Kusworini, Bambang Pardjianto. Biological Activity of Papain and Papain-like (Cathepsin-K and Cathepsin-B) Enzymes as Therapeutical Modality Candidates in Degrading Collagen in Abnormal Scar. Research Journal of Pharmacy and Technology. 2021; 14(9):4957-2. doi: 10.52711/0974-360X.2021.00862

Herman Y. L. Wihastyoko, Setyawati Soeharto, Edi Widjajanto, Kusworini, Bambang Pardjianto. Biological Activity of Papain and Papain-like (Cathepsin-K and Cathepsin-B) Enzymes as Therapeutical Modality Candidates in Degrading Collagen in Abnormal Scar. Research Journal of Pharmacy and Technology. 2021; 14(9):4957-2. doi: 10.52711/0974-360X.2021.00862   Available on:

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