Author(s): Rasha Eldalawy, Widad M K Al-Ani, Wasan Abdul Kareem

Email(s): ,

DOI: 10.52711/0974-360X.2021.00794   

Address: Rasha Eldalawy1*, Widad M K Al-Ani2, Wasan Abdul Kareem3
1Pharmacognosy and Medicinal Plants Department, College of Pharmacy, Mustansiriyah University, Baghdad – Iraq.
2Ashur University College, Baghdad- Iraq.
3Clinical Laboratory Science Department, College of Pharmacy, Mustansiriyah University, Baghdad – Iraq.
*Corresponding Author

Published In:   Volume - 14,      Issue - 9,     Year - 2021

Milk thistle's extract by an organic solvent is known as silymarin, which is a mixture of about 75% flavolignan and trace content of flavonoid (mainly taxifolin), other ingredients involve fatty acids and polyphenolic compounds. The major flavolignans are silybin, isosilybin, silydianin, and silychristin, quantitatively silybin is considered as the major compound followed by isosilybin, and both are represent approximately 60% of silymarin. This study was designed to determine the concentration of silybin in different parts of the plant and to study the effect of extraction methods, solvents and the time of collection on silybin concentration, which have been done by HPLC instrument. The results show that the higher yield of silybin is obtained from the seed using ethanol in the soxhlet apparatus and that all other parts of the plant which are produced in huge amount by the plant also contain a good concentration of silybin when compared with seeds, So they can be used as an alternative source for silybin production.

Cite this article:
Rasha Eldalawy, Widad M K Al-Ani, Wasan Abdul Kareem. Quantitative estimation of Silybin in Iraqi Silybum marianum by HPLC. Research Journal of Pharmacy and Technology. 2021; 14(9):4567-2. doi: 10.52711/0974-360X.2021.00794

Rasha Eldalawy, Widad M K Al-Ani, Wasan Abdul Kareem. Quantitative estimation of Silybin in Iraqi Silybum marianum by HPLC. Research Journal of Pharmacy and Technology. 2021; 14(9):4567-2. doi: 10.52711/0974-360X.2021.00794   Available on:

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