Ganadhal Puttaramaiah Chethankumara, Venkatarangaiah Krishna, Kakanahalli Nagaraj
Ganadhal Puttaramaiah Chethankumara1, Venkatarangaiah Krishna2, Kakanahalli Nagaraj1*
1Department of PG Studies and Research in Applied Zoology, Kuvempu University, Shivamogga, Karnataka, 577 451.
2Department of PG Studies and Research in Biotechnology, Kuvempu University, Shivamogga, Karnataka, 577 451.
Volume - 15,
Issue - 1,
Year - 2022
A. semecarpifolia (Lauraceae) has been employed by Indian traditional healers to treat various human cancers. The cytotoxic property of A. semecarpifolia was evaluated by extracting secondary metabolites from stem bark and leaves. The stem bark methanol extract (SBME) and leaf methanol extract (LME) were subjected to HR-LCMS analysis, followed by evaluating their cytotoxic potential against MCF-7, SCC-9, HeLa, A-549 and L6 cells by MTT assay. The phytochemical screening of SBME and LME revealed the presence of alkaloids, flavonoids, terpenoids, steroids, saponins, phenolics and glycosides. HR-LCMS analysis of SBME and LME revealed the presence of 31 and 28 active compounds respectively. Among them Cucurbitacin A and Gambogic acid are the two compounds identified with anticancer properties. SBME showed potent cytotoxicity on MCF-7 and SCC-9 cells, whereas LME showed significant cytotoxicity only on MCF-7 cells. Vinblastine sulphate was used as a reference standard, even though Vinblastine is an efficient anticancer drug it affected the viability of normal cells. In comparison with Vinblastine, both SBME and LME showed very less toxicity on normal cells. Hence, the present study suggested that stem bark and leaves of A. semecarpifolia are the possible chemotherapeutic agents having potential cytotoxic activity against MCF-7 and SCC-9 cells.
Cite this article:
Ganadhal Puttaramaiah Chethankumara, Venkatarangaiah Krishna, Kakanahalli Nagaraj. HR-LCMS and In vitro cytotoxicity analysis of Alseodaphne semecarpifolia stem bark and leaf methanol extracts. Research Journal of Pharmacy and Technology. 2022; 15(1):250-6. doi: 10.52711/0974-360X.2022.00041
Ganadhal Puttaramaiah Chethankumara, Venkatarangaiah Krishna, Kakanahalli Nagaraj. HR-LCMS and In vitro cytotoxicity analysis of Alseodaphne semecarpifolia stem bark and leaf methanol extracts. Research Journal of Pharmacy and Technology. 2022; 15(1):250-6. doi: 10.52711/0974-360X.2022.00041 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-1-41
1. Iqbal J, Abbasi BA, Mahmood T, Kanwal S, Ali B, Shah SA, et al. Plant-derived anticancer agents: A green anticancer approach. Asian Pacific Journal of Tropical Biomedicine. 2017; 7(12): 1129-1150.
2. Vijayabaskaran M, Sivakumar P, Sambathkumar R, Perumal P, Sivakumar T, Jayakar B. Anti-tumor and anti-oxidant activities of Pseudarthria viscida against Dalton's ascites lymphoma bearing Swiss albino mice. Research Journal of Pharmacy and Technology. 2008; 1(3): 225-229.
3. Kamboh AA, Arian MA, Mughal MJ, Zaman A, Arain ZM, Soomro AH. Flavonoids: health promoting phytochemicals for animal production - A review. Journal of Animal Health and Production. 2015; 3(1): 6-13.
4. Mukherjee PK, Nema NK, Bhadra M, Mukherjee D, Braga FC, Matsabisa MG. Immunomodulatory leads from medicinal plants. Indian Journal of Traditional Knowledge. 2014; 13(2): 235-256.
5. Jain S, Dwivedi J, Jain PK, Satpathy S, Patra A. Medicinal plants for treatment of cancer: A brief review. Pharmacognosy Journal. 2016; 8(2): 87-102.
6. Lichota A, Gwozdzinski K. Anticancer Activity of Natural Compounds from Plant and Marine Environment. International Journal of Molecular Sciences. 2018; 19: 1-38.
7. Jagtap NS, Nalamwar VP, Khadabadi SS, Pratapwar AS. Phytochemical and Pharmacological Profile of Annona squamosa Linn: A Review. Research Journal of Pharmacognosy and Phytochemistry. 2009; 1(3): 139-142.
8. Fulda S. Modulation of apoptosis by natural products for cancer therapy. Planta Medica. 2010; 76(11): 1075-1079.
9. Arora R, Malhotra P, Chawla R, Gupta D, Sharma RK, Baliga MS. Indian Herbal Medicine for Cancer Therapy and Prevention. Bioactive Foods and Extracts. 2010; 519-543.
10. Tripuramallu S, Sunkara S, Reddy VR. Cytotoxic Activity of Glocihdium zeylanicum Leaf Extract. Research Journal of Pharmacology and Pharmacodynamics. 2009; 1(3): 145-146.
11. Verma RS, Kumar A, Mishra P, Kuppusamy B, Padalia RC, Sundaresan V. Essential oil constituents of Alseodaphne semecarpifolia from Central Western Ghats, India. Chemistry of Natural Compounds. 2016; 52(3): 516-517.
12. Harborne JB. Phytochemical methods: A guide to modern techniques of plant analysis. London: Chapman and Hall. 2005.
13. Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology. 2005; 4(7): 685-688.
14. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry. 1999; 64(4): 555-559.
15. Senguttuvan J, Paulsamy S, Karthika K. Phytochemical analysis and evaluation of leaf and root parts of the medicinal herb, Hypochaeris radicata L. for in vitro antioxidant activities. Asian Pacific Journal of Tropical Biomedicine. 2014; 4(1): 359-367.
16. Makkar HP, Siddhuraju P, Becker K. Methods in molecular biology: plant secondary metabolites. Totowa: Human Press. 2007; 93-100.
17. Siddhuraju P, Becker K. Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera lam.) Leaves. Journal of Agricultural and Food Chemistry. 2003; 51: 2144-2155.
18. Durai MV, Balamuniappan G, Anandalakshmi R, Geetha S, Kumar NS. Qualitative and quantitative analysis of phytochemicals in crude extract of big - leaf Mahogany (Swietenia macrophylla King). International Journal of Herbal Medicine. 2016; 4(6): 88-91.
19. Singh R, Verma PK, Singh G. Total phenolic, flavonoids and tannin contents in different extracts of Artemisia absinthium. Journal of Intercultural Ethnopharmacology. 2012; 1(2): 101-104.
20. Malik SK, Ahmad M, Khan F. Qualtitative and quantitative estimation of terpenoid contents in some important plants of Punjab, Pakistan. Pakistan Journal of Science. 2017; 69(2): 150-154.
21. Indumathi C, Durgadevi G, Nithyavani S, Gayathri PK. Estimation of terpenoid content and its antimicrobial property in Enicostemma litorrale. International Journal of ChemTech Research. 2014; 6(9): 4264-4267.
22. Devanaboyina N, Ramalakshmi N, Satyanarayana, Sudeepthi P, Chakradhar KH, Raju NPK. Preliminary phytochemical screening, quantitative estimation and evaluation of antimicrobial activity of Alstonia macrophylla stem bark. International Journal of Science Inventions Today. 2013; 2(1): 31-39.
23. Ajiboye BO, Ibukun EO, Edobor G, Ojo AO, Onikanni SA. Qualitative and quantitative analysis of phytochemicals in Senecio biafrae leaf. International Journal of Inventions in Pharmaceutical Sciences. 2013; 1(5): 428-432.
24. El-olemy MM, Al-muhtadi FJ, Affi AFA. Experimental Phytochemical: A Laboratory Manual, King Saud University Press. 1994; Saudi Arabia.
25. Alley MC, Scudiere DA, Monks A, Hursey ML, Czerwinski MJ, Fine DL, et al. Feasibility of Drug Screening with Panels of Human Tumor Cell Lines Using a Microculture Tetrazolium Assay. Cancer Research. 1988; 4: 589-601.
26. Abdullah AH, Mohammed AS, Abdullah R, Mirghani MES, Al-Qubaisi M. Cytotoxic effects of Mangifera indica L. kernel extract on human breast cancer (MCF-7 and MDA MB-231 cell lines) and bioactive constituents in a crude extract. BMC Alternative Medicine. 2014; 14(199): 1-10.
27. Ren Y, Yuan C, Chai H, Ding Y, Li XC, Ferreira D et al. Absolute Configuration of (-)-Gambogic Acid, an Antitumor Agent. Journal of Natural Products. 2011; 74: 460-463.
28. Ali MS, Mukherjee S, Makar S, Pal G. Cucurbitacins a Vibrant Triterpenoid: A Review on its Anticancer Property. PharmaTutor. 2019; 7(2): 43-54.
29. Wang J, Zhao L, Hu Y, Guo Q, Zhang L, Wang X, et al. Studies on chemical structure modification and biology of a natural product, Gambogic acid (I): Synthesis and biological evaluation of oxidized analogues of gambogic acid. European Journal of Medicinal Chemistry. 2009; 44: 2611-2620.
30. Wen C, Huang L, Chen J, Lin M, Li W, Lu B et al. Gambogic acid inhibits growth, induces apoptosis, and overcomes drug resistance in human colorectal cancer cells. International Journal of Oncology. 2015; 47: 1663-1671.
31. Yuan H, Ma Q, Ye L, Piao G. The Traditional Medicine and Modern Medicine from Natural Products. Molecules. 2016; 21: 559.
32. Saha D, Paul S. Pharmacognostic Studies of Aerial Part of Methanolic Extract of Alpinia conchigera Griff. Asian Journal of Pharmaceutical Analysis. 2012; 2(2): 46-48.
33. Anand U, Herrera NJ, Altemimi A, Lakhssassi N. A Comprehensive Review on Medicinal Plants as Antimicrobial Therapeutics: Potential Avenues of Biocompatible Drug Discovery. Metabolites. 2019; 9(258): 1-13.
34. Parthasarathy R, Ilavarasan R, Karrunakaran CM. Pharmacognostical Studies on Thespesia populnea Bark. Research Journal of Pharmacognosy and Phytochemistry. 2009; 1(2): 128-131.
35. Akter R, Gealt MA, Kleve MG, Hossain Z. Cytotoxicity of Wortmannin triggers programmed cell death in MCF-7 cells; biochemical and morphological analysis. Journal of Cancer Prevention and Current Research. 2016; 4(4): 1-7.
36. Ferreira JRO, Cavalcanti BC, da-Costa PM, de-Arantes FFP, de-Alvarenga ES, Maltha CRA. et al. Induction of G2/M arrest, caspase activation and apoptosis by a-santonin derivatives in HL-60 cells. Toxicology In vitro. 2013; 25: 1458-1466.
37. Pandey MK, Sung B, Ahn KS, Kunnumakkara AB, Chaturvedi MM, Aggarwal BB. Gambogic acid, a novel ligand for transferrin receptor, potentiates TNF-induced apoptosis through modulation of the nuclear factor-κB signaling pathway. Blood. 2007; 110(10): 3517-3525.