Author(s): Sylvia Viana Varanita, Hartono Kahar, Munawaroh Fitriah

Email(s): hartonokahar@yahoo.co.id

DOI: 10.52711/0974-360X.2022.00745   

Address: Sylvia Viana Varanita1, Hartono Kahar2*, Munawaroh Fitriah2
1Clinical Pathology Specialization Programme, Faculty of Medicine, Airlangga University, Dr. Soetomo Hospital, Surabaya, Indonesia.
2Department of Clinical Pathology, Faculty of Medicine, Airlangga University, Dr. Soetomo Hospital, Surabaya, Indonesia.
*Corresponding Author

Published In:   Volume - 15,      Issue - 10,     Year - 2022


ABSTRACT:
Routine Hemoglobin (Hb) and Hematocrit (Hct) tests are done with the Sysmex XN 1000 device at the Central Laboratory of Dr. Soetomo General Hospital. Aptus POCT can be used quickly and practically for rapid Hb and Hct tests. Intherma EDTA tubes are usually used to substitute the empty supply of Greiner EDTA tubes for blood sampling. This study aims to analyze Hb and Hct levels from blood samples in Greiner and Intherma EDTA tubes which are measured using Aptus and Sysmex devices, as well as analyze their correlation. This study is a descriptive analytic study with general check-up outpatients in Dr. Soetomo General Hospital since January until May 2020 as the research subjects (n = 50). Research variables consist of Intherma tubes, Greiner tubes, Hb and Hct. Statistical analysis was performed to compare Hb, Hct values using Intherma tubes and Greiner tubes which used Aptus and Sysmex XN 1000 devices. Results revealed that mean Hb value using Greiner tubes and Sysmex device was 13.73 ± 1.39, mean Hb value using Greiner tubes and Aptus device was 12.9 ± 1.1, mean Hb value using Intherma tube and Sysmex device was 13.9 ± 1.513 and mean Hb value using Intherma tube and Aptus device was 13.00 ± 1.112. Adjustments are required for the Aptus device, which still uses basic methods unlike the Sysmex XN 1000 device which is a reference value. In conclusion, there is a significant difference in Hb levels measured using Sysmex and Aptus devices. There is also a significant difference in Hct levels measured using Greiner and Intherma EDTA tubes.


Cite this article:
Sylvia Viana Varanita, Hartono Kahar, Munawaroh Fitriah. Compatibility of Two EDTA Tubes for Hemoglobin (Hb) Hematocrit (Hct) Tests with Aptus Equipment. Research Journal of Pharmacy and Technology2022; 15(10):4447-0. doi: 10.52711/0974-360X.2022.00745

Cite(Electronic):
Sylvia Viana Varanita, Hartono Kahar, Munawaroh Fitriah. Compatibility of Two EDTA Tubes for Hemoglobin (Hb) Hematocrit (Hct) Tests with Aptus Equipment. Research Journal of Pharmacy and Technology2022; 15(10):4447-0. doi: 10.52711/0974-360X.2022.00745   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-10-18


REFERENCES:
1.    Subbalakshmi NK, Sunandha S. Influence of Perceived Stress on Hemoglobin Concentration. Research J. Pharm. and Tech. 2017; 10(1): 61-64. DOI: 10.5958/0974-360X.2017.00015.4
2.    Rana I. Ahmad, Haider A. Al-Barry, Salwa I. Salloum. Fetal Hemoglobin (HbF) levels in Sickle cell anemia patients in Lattakia, Syria. Research J. Pharm. and Tech. 2020; 13(7): 3246-3248. DOI: 10.5958/0974-360X.2020.00575.2
3.    Merlin Abraham, Lissa J, Sheela Williams. A Correlation Study to Assess the Relationship of Menstrual Irregularities, body Mass Index (BMI) and Hemoglobin (HB) level among Adolescent Girls in Selected College at Mysuru. Int. J. Nur. Edu. and Research. 2018; 6(1): 101-106. DOI: 10.5958/2454-2660.2018.00025.X
4.    Ruheena Yasmeen, N. Krishna Reddy, Marina. G. D’ Souza, B. Swathi, Anas Abdul Waheed, Katakam Chinmayee, J. Vineeth Reddy. Study of Anticoagulants low molecular weight heparin and Unfractionated heparin in the management of Non-St elevation Myocardial Infarction. Research J. Pharm. and Tech. 2020; 13(7): 3151-3155. DOI: 10.5958/0974-360X.2020.00557.0
5.    Talib Abdullah Hussein1, Anwar Salih Saihood2, Ahmed Raheem Rayshan. Molecular Identification and Characterization in blood samples of patients with progressive multifocal leukoencephalopathy and human kidney cell line culture of John Cunningham Virus (JCV) in Iraq. Research J. Pharm. and Tech 2018; 11(8): 3392-3398. DOI: 10.5958/0974-360X.2018.00625.X
6.    Ogugua Victor N., Emmanuel Tufon N., Enechi Osmond C. Effect of Vitamin E-Stored Experimental Blood Samples on Malondialdehyde and Protein Concentration. Research J. Pharm. and Tech. 4(11): Nov. 2011; Page 1785-1786.
7.    M. P. Brundha, Pathmashri. V. P, Shantha Sundari. Quantitative Changes of Red Blood cells in Cancer Patients under Palliative Radiotherapy-A Retrospective Study. Research J. Pharm. and Tech 2019; 12(2):687-692. DOI: 10.5958/0974-360X.2019.00122.7
8.    Suneetha. P.. A Study to Assess the Effectiveness of Structured Teaching Programme on Blood Transfusion among Student Nurses in Selected Nursing Institution at Raichur. Asian J. Nur. Edu. & Research 1(1): Jan.-March 2011; Page 12-14.
9.    Preethi Jazna.B, Reeta Jebakumari, Nalini Jeyavantha Santha. A Study to Assess the Effectiveness of Benson Relaxation Therapy on Blood Pressure and Stress among Women with Pregnancy Induced Hypertension in Selected Hospitals, Madurai. Asian J. Nur. Edu. and Research. 2016; 6(2): 167-170. DOI: 10.5958/2349-2996.2016.00031.8
10.    Vivitha S. Pinto, Pushpaveni N. P. A study to Evaluate the effectiveness of Structured Teaching Program regarding collection of cord blood for stem cell therapy in terms of knowledge among staff nurses in selected hospitals at Bangalore. Asian J. Nursing Education and Research. 2018; 8(1): 07-10. DOI: 10.5958/2349-2996.2018.00003.4
11.    Monago, C. C., Nwiko, E. B., Chuku, L. C. . Assessment of Polycyclic Aromatic Hydrocarbon Levels in Blood of Refinery Workers in Nigeria. Asian J. Research Chem. 3(3): July- Sept. 2010; Page 801-804.
12.    V. Komathi. Effectiveness of Guided Imagery on Level of Blood Pressure among PIH Mothers in Selected Hospital at Trichy. Int. J. Adv. Nur. Management 3(3): July- Sept. 2015; Page 245-252.
13.    M. Chitra, D. Karthikeyan. Mathematical Modelling on Oscillatory flow of Blood in a stenosed artery under the influence of Magnetic field with Variable Viscosity. Research J. Science and Tech. 2017; 9(3):427-434. DOI: 10.5958/2349-2988.2017.00075.4
14.    Gari MA. The Comparison of glass EDTA versus plastic EDTA blood-drawing tubes for complete blood count. Middle-East Journal of Scientific Res. 2008; 3(1): 32–35.
15.    Bowen RAR, Remaley AT. Interferences from blood collection tube components on clinical chemistry assays. Biochemia Medica. 2014; 24(1): 31–44.
16.    Preissner CM, Reilly WM, Cyr RC, O’Kane DJ, Singh RJ, Grebe SKG. Plastic versus glass tubes: Effects on analytical performance of selected serum and plasma hormone assays. Clinical Chemistry. 2004; 50(7): 1245–1247.
17.    NCCLS. Tubes and additives for venous blood specimen collection; Approved standard — Fifth edition. NCCLS Document H1-A5. 2003; 23(33): 1-31.
18.    Seo JY, Lee ST, Kim SH. Performance evaluation of the new hematology analyzer Sysmex XN-series. Int J Lab Hematol. 2015 Apr; 37(2):155-64. doi: 10.1111/ijlh.12254
19.    de Bitencourt ED, Voegeli CF, Onzi Gdos S, Boscato SC, Ghem C, Munhoz T. Validation of the Sysmex sp-1000i automated slide preparer-stainer in a clinical laboratory. Rev Bras Hematol Hemoter. 2013; 35(6):404-8. doi: 10.5581/1516-8484.20130121
20.    Schoorl M, Schoorl M, Chevallier M, van der Ploeg T, van Pelt J. Multicenter verification of the Sysmex XN-Series. Int J Lab Hematol. 2017 Oct; 39(5):489-496. doi: 10.1111/ijlh.12674
21.    Dumas C, Bienvenu AL, Girard S, Picot S, Debize G, Durand B. Automated Plasmodium detection by the Sysmex XN hematology analyzer. J Clin Pathol. 2018 Jul; 71(7):594-599. doi: 10.1136/jclinpath-2017-204878
22.    Petersson A, Ekblom K. Evaluation of a routine hematology analyzer for quality control of leukoreduced plasma. Transfusion. 2019 Oct; 59(10):3214-3218. doi: 10.1111/trf.15481
23.    Mohapatra S, Samantaray JC, Arulselvi S, Panda J, Munot K, Saxena R. Automated detection of malaria with haematology analyzer Sysmex XE-2100. Indian J Med Sci. 2011 Jan; 65(1):26-31
24.    Srivastava A, Koul V, Dwivedi SN, Upadhyaya AD, Ahuja A, Saxena R. Performance analysis of newly developed point-of-care hemoglobinometer (TrueHb) against an automated hematology analyzer (Sysmex XT 1800i) in terms of precision in hemoglobin measurement. Int J Lab Hematol. 2015 Aug; 37(4):483-5. doi: 10.1111/ijlh.12314
25.    Briggs C, Kunka S, Pennaneach C, Forbes L, Machin SJ. Performance evaluation of a new compact hematology analyzer, the Sysmex pocH-100i. Lab Hematol. 2003; 9(4):225-33.
26.    Briggs C, Longair I, Kumar P, Singh D, Machin SJ. Performance evaluation of the Sysmex haematology XN modular system. J Clin Pathol. 2012 Nov; 65(11):1024-30. doi: 10.1136/jclinpath-2012-200930
27.    Yoo DW, Lee SM, Moon SY, Kim IS, Chang CL. Evaluation of conductivity-based osmolality measurement in urine using the Sysmex UF5000. J Clin Lab Anal. 2021 Jan; 35(1):e23586. doi: 10.1002/jcla.23586

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

1.3
2021CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags


Not Available