Author(s): Revi Gama Hatta Novika, Cahyaning Setyo Hutomo, Nurul Jannatul Wahidah, Lanjar Sumarno, Nanda Yuli Rahmawati, Arif Nur Muhammad Ansori, Ahmad Yunus

Email(s): revi.gama@staff.uns.ac.id

DOI: 10.52711/0974-360X.2022.00748   

Address: Revi Gama Hatta Novika1*, Cahyaning Setyo Hutomo1, Nurul Jannatul Wahidah1, Lanjar Sumarno2, Nanda Yuli Rahmawati3, Arif Nur Muhammad Ansori4, Ahmad Yunus5
1Midwifery Study Program, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia.
2National Research and Innovation Agency, Jakarta, Indonesia.
3Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
4Professor Nidom Foundation, Surabaya, Indonesia.
5Department of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta, Indonesia.
*Corresponding Author

Published In:   Volume - 15,      Issue - 10,     Year - 2022


ABSTRACT:
Progesterone-induced blocking factor (PIBF) is crucial to maintain pregnancy and the mother’s progesterone immunomodulation. Herbal medicine is widely used to induce immunity and treat pregnancy-related problems. Apigenin is a flavonoid type from Apium graveolens and could be a potential herbal medicine. The study aimed to evaluate the effective dose of A. graveolens in pregnant mice’s PIBF level on preclinical research and pregnant women on clinical research. Preclinical research used 32 pregnant mice divided into four groups randomly. The first group was the control group (P1), while P2, P3, and P4 groups were the treatment group. Pregnant mice were given 1mg/g (P2), 2mg/g (P3), and 4mg/g (P4) of A. graveolens extract orally for 14 days. Clinical research was completely randomized design. Thirty pregnant women at Mrs. Sri Lumintu Clinic, Surakarta, Indonesia divided into two groups. The treatment group was given A. graveolens capsule 534mg/50 kg BW, Fe, and calcium, while the control group was given Placebo, Fe, and calcium orally for 14 days. PIBF level in both preclinical and clinical was determined using enzyme-linked immunosorbent assay (ELISA). Our study showed that A. graveolens with 2mg/g (P3) was the most effective dose to increase PIBF level in pregnant mice. The PIBF level of pregnant women increased significantly in the treatment group compared to the control group (444.78pg/mL and 171.74pg/mL, p = 0.000). A. graveolens supplementation in pregnant women increased PIBF levels during pregnancy and could prevent inflammation during pregnancy.


Cite this article:
Revi Gama Hatta Novika, Cahyaning Setyo Hutomo, Nurul Jannatul Wahidah, Lanjar Sumarno, Nanda Yuli Rahmawati, Arif Nur Muhammad Ansori, Ahmad Yunus. The Effect of Apium graveolens L. in Progesterone-Induced Blocking Factor (PIBF) During Pregnancy. Research Journal of Pharmacy and Technology2022; 15(10):4463-8. doi: 10.52711/0974-360X.2022.00748

Cite(Electronic):
Revi Gama Hatta Novika, Cahyaning Setyo Hutomo, Nurul Jannatul Wahidah, Lanjar Sumarno, Nanda Yuli Rahmawati, Arif Nur Muhammad Ansori, Ahmad Yunus. The Effect of Apium graveolens L. in Progesterone-Induced Blocking Factor (PIBF) During Pregnancy. Research Journal of Pharmacy and Technology2022; 15(10):4463-8. doi: 10.52711/0974-360X.2022.00748   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-10-21


REFERENCES:
1.    Quenby S, Gallos ID, Dhillon-Smith RK, Podesek M, Stephenson MD, Fisher J, Brosens JJ, Brewin J, Ramhorst R, Lucas ES, McCoy RC, Anderson R, Daher S, Regan L, Al-Memar M, Bourne T, MacIntyre DA, Rai R, Christiansen OB, Sugiura-Ogasawara M, Odendaal J, Devall AJ, Bennett PR, Petrou S, Coomarasamy A. Miscarriage matters: The epidemiological, physical, psychological, and economic costs of early pregnancy loss. Lancet. 2021; 397(10285): 1658-1667. doi: 10.1016/S0140-6736(21)00682-6.
2.    The American College of Obstetricians and Gynecologists Practice Bulletin no. 150. Early pregnancy loss. Obstet Gynecol. 2015; 125(5): 1258-1267. doi: 10.1097/01.AOG.0000465191.27155.25.
3.    Kanmaz AG, İnan AH, Beyan E, Budak A. The effects of threatened abortions on pregnancy outcomes. Ginekol Pol. 2019; 90(4): 195-200. doi: 10.5603/GP.a2019.0035.
4.    Zainab Jawad Naki, Dalal Abdul Hussain Kadium, Buthyna Abd el-hassan Naser, Zainab Mahdi Jasim Al-Shammari. Effect of alcoholic extract of celery seeds Apium graveolens on the histological characteristic ovaries of swiss rat ratus. Res J Pharm Tech. 2018; 11(1): 193-198. doi: 10.5958/0974-360X.2018.00036.7
5.    Rouhi-Boroujeni H, Heidarian E, Rouhi-Boroujeni H, Khoddami M, Gharipour M, Rafieian-Kopaei M. Use of lipid-lowering medicinal herbs during pregnancy: A systematic review on safety and dosage. ARYA Atheroscler. 2017; 13(3): 135-155.
6.    Jung WS, Chung IM, Kim SH, Kim MY, Ahmad A, Praveen N. In vitro antioxidant activity, total phenolics and flavonoids from celery (Apium graveolens) leaves. J Med Plants Res. 2011; 5: 7022-7030.
7.    Ganesh MS, Radhika J. Evaluation of quantitative and GC-MS analysis of bioactive compounds in aqueous and ethanolic extracts of Apium leptophyllum Pers. Res J Pharm Tech. 2020; 13(5): 2087-2090. doi: 10.5958/0974-360X.2020.00375.3
8.    Jadhav CA, Vikhe DN, Jadhav RS. Global and domestic market of herbal medicines: A review. Res J Sci Tech. 2020; 12(4): 327-330. doi: 10.5958/2349-2988.2020.00049.2
9.    Peng J, Li R, Yin H, Tang F, Xie H, Li M, Zhao Y. A case report of a pregnant woman infected with coronavirus disease 2019 pneumonia. Medicine. 2020; 99(30): e21335. doi: 10.1097/MD.0000000000021335.
10.    Kishor RS, Ramhari BM. Introduction to Covid-19. Res J Sci Tech. 2020; 12(4): 338-345. doi: 10.5958/2349-2988.2020.00051.0
11.    Wang L, Shi Y, Xiao T, Fu J, Feng X, Mu D, Feng Q, Hei M, Hu X, Li Z, Lu G, Tang Z, Wang Y, Wang C, Xia S, Xu J, Yang Y, Yang J, Zeng M, Zheng J, Zhou W, Zhou X, Zhou X, Du L, Lee SK, Zhou W; Working Committee on Perinatal and Neonatal Management for the Prevention and Control of the 2019 Novel Coronavirus Infection. Chinese expert consensus on the perinatal and neonatal management for the prevention and control of the 2019 novel coronavirus infection (First edition). Ann Transl Med. 2020; 8(3): 47. doi: 10.21037/atm.2020.02.20.
12.    Rohit Kumar, Anshul Chawla, Gaganpreet, Diksha. A Valuable insight to the novel deadly COVID-19: A review. Res J Pharmacol Pharmacodyn. 2020; 12(3): 111-116. doi: 10.5958/2321-5836.2020.00021.X
13.    Szekeres-Bartho J. The role of progesterone in feto-maternal immunological cross talk. Med Princ Pract. 2018;27(4):301-307. doi: 10.1159/000491576.
14.    Shikha Baghel Chauhan. Post-coital Anti-implantation and antifertility activity of transdermal drug delivery of ethinylestradiol and medroxy progesterone acetate. Res J Pharm Tech. 2020; 13(5): 2255-2260. doi: 10.5958/0974-360X.2020.00406.0
15.    Szekeres-Bartho J, Polgar B. PIBF: The double edged sword. Pregnancy and tumor. Am J Reprod Immunol. 2010; 64(2): 77-86. doi: 10.1111/j.1600-0897.2010.00833.x.
16.    Hudić I, Fatusić Z, Szekeres-Bartho J, Balić D, Polgar B, Ljuca D, Dizdarević-Hudić L. Progesterone-induced blocking factor and cytokine profile in women with threatened pre-term delivery. Am J Reprod Immunol. 2009; 61(5): 330-7. doi: 10.1111/j.1600-0897.2009.00699.x.
17.    Raghupathy R, Al-Mutawa E, Al-Azemi M, Makhseed M, Azizieh F, Szekeres-Bartho J. Progesterone-induced blocking factor (PIBF) modulates cytokine production by lymphocytes from women with recurrent miscarriage or preterm delivery. J Reprod Immunol. 2009; 80(1-2): 91-9. doi: 10.1016/j.jri.2009.01.004.
18.    Szekeres-Bartho J, Šućurović S, Mulac-Jeričević B. The role of extracellular vesicles and PIBF in embryo-maternal immune-interactions. Front Immunol. 2018; 9: 2890. doi: 10.3389/fimmu.2018.02890.
19.    Naeem Hasan Khan, Nabila Perveen, Azmi Sarriff. A study on herbal drugs utilization in pregnant women from hospitals of Sungai Petani, Kedah Darul Aman (D.A.), Malaysia. Res J Pharm Tech. 2018; 11(3): 969-975. doi: 10.5958/0974-360X.2018.00181.6.
20.    Csabai T, Pallinger E, Kovacs AF, Miko E, Bognar Z, Szekeres-Bartho J. Altered immune response and implantation failure in progesterone-induced blocking factor-deficient mice. Front. Immunol. 2020; 11: 349. doi: 10.3389/immu.2020.00349.
21.    Yogita Hiwarkar, Amit Hiwarkar. An analytical study of factors related to reproductive health. Res J Pharmacol Pharmacodyn. 2013; 5(1): 43-47.
22.    Liu J, Dong P, Jia N, Wen X, Luo L, Wang S, Li J. The expression of intracellular cytokines of decidual natural killer cells in unexplained recurrent pregnancy loss. J Matern Fetal Neonatal Med. 2020: 1-7. doi: 10.1080/14767058.2020.1817369.
23.    Gauri M, Javed Ali S, Shahid Khan M. A review of Apium graveolens (Karafs) with special reference to Unani medicine. Int Arch Integr Med. 2015; 2: 131–136.
24.    Kooti W, Ali-Akbari S, Asadi-Samani M, Ghadery H, Ashtary-Larky D. A review on medicinal plant of Apium graveolens. Adv Herb Med. 2014; 1: 48–59.
25.    Sreesha N Nair, Neeraja E D, Greeshma Rejimon, Meenu B, Alexeyena Varghese. Comparative evaluation of the anthelmintic activity of Coriandrum sativum Linn. and Apium graveolens Linn. Res J Pharm Tech. 2017; 10(11): 3857-3859. doi: 10.5958/0974-360X.2017.00699.0
26.    Sreesha N Nair, Alexeyena Varghese, Meenu B, Greeshma Rejimon, Neeraja E D. Comparative evaluation of Coriandrum sativum Linn. and Apium graveolens for antimicrobial activity. Res J Pharm Tech. 2017; 10(2): 541-544. doi: 10.5958/0974-360X.2017.00108.1
27.    Kooti W, Daraei N. A review of the antioxidant activity of celery (Apium graveolens L). J Evid Based Complementary Altern Med. 2017; 22(4): 1029-1034. doi: 10.1177/2156587217717415.
28.    Shabnian et al. The medicinal plants effective on female hormones: A review of the native medicinal plants of Iran effective on estrogen, progesterone, and prolactin. J Chem Pharma Sci. 2015; 9: 3.
29.    Heidarifar R, Farahani H, Mirizadeh M, Yousefi A, Dolatshahi M, Kazemian Mansourabad M, The effect of hydroalcoholic extract of Anethum graveolens (Dill) on serum estrogen and progesterone level in female rats. Qom Univ Med Sci J. 2015; 9(5): 42-49.
30.    Jambor T, Arvay J, Tvrda E, Kovacik A, Greifova H, Lukac N. The effect of Apium graveolens L., Levisticum officinale and Calendula officinalis L. on cell viability, membrane integrity, steroidogenesis, and intercellular communication in mice Leydig cells in vitro. Physiol Res. 2021; 70(4): 615-625. doi: 10.33549/physiolres.934675.
31.    Chiarello DI, Abad C, Rojas D, Toledo F, Vázquez CM, Mate A, Sobrevia L, Marín R. Oxidative stress: Normal pregnancy versus preeclampsia. Biochim Biophys Acta Mol Basis Dis. 2020; 1866(2): 165354. doi: 10.1016/j.bbadis.2018.12.005
32.    Naid et al. Effect of Alcoholic extract of celery seed Apium graveolens on th ehistological characteristic ovaries of swiss Rat ratus. J Pharm Tech. 2018; 11(1).
33.    El-Zaher HM, Eid SY, Shaaban MM, Ahmed-Farid OA, Abd El Tawab AM, Khattab MSA. Ovarian activity and antioxidant indices during estrous cycle of Barki ewes under effect of thyme, celery and salinomycin as feed additives. Zygote. 2021; 29(2): 155-160. doi: 10.1017/S0967199420000611
34.    Solano ME, Arck PC. Steroids, pregnancy and fetal development. Front Immunol. 2020; 10: 3017. doi: 10.3389/fimmu.2019.03017.
35.    Shah NM, Lai PF, Imami N, Johnson MR. Progesterone-Related Immune Modulation of Pregnancy and Labor. Front Endocrinol. 2019; 10: 198. doi: 10.3389/fendo.2019.00198.
36.    Lim MK, Ku CW, Tan TC, Lee YHJ, Allen JC, Tan NS. Characterisation of serum progesterone and progesterone-induced blocking factor (PIBF) levels across trimesters in healthy pregnant women. Sci Rep. 2020; 10(1): 3840. doi: 10.1038/s41598-020-59452-y
37.    Hardani A, Afzalzadeh MR, Amirzargar A, Mansouri E, Meamar Z. Effects of aqueous extract of celery (Apium graveolens L.) leaves on spermatogenesis in healthy male rats. Avicenna J Phytomed. 2015; 5(2): 113-9.
38.    Njoku Ugochi U, et al. Antioxidant properties of Apium graveolens. Res J Pharm Phytochem. 2011; 3(5): 201-205.
39.    Shah NM, Imami N, Johnson MR. Progesterone modulation of pregnancy-related immune responses. Front Immunol. 2018; 9: 1293. doi: 10.3389/fimmu.2018.01293
40.    Khairullah AR, Solikhah TI, Ansori ANM, Hidayatullah AR, Hartadi EB, Ramandinianto SC, Fadholly A. Review on the pharmacological and health aspects of Apium graveolens or celery: An update. Syst Rev Pharm. 2021; 12(1): 606-612. doi: 10.31838/srp.2021.1.87
41.    Gutierrez-Rodríguez A, Camacho-Arroyo I. Role of progesterone-induced blocking factor (PIBF) in pregnancy and cancer. TIP Rev Esp Cienc Quim Biol. 2016; 19(2): 109-115.
42.    Hajipour H, Nejabati HR, Latifi Z, Hamdi K, Bahrami-Asl Z, Fattahi A, Nouri M. Lymphocytes immunotherapy for preserving pregnancy: Mechanisms and challenges. Am J Reprod Immunol. 2018; 80(3): e12853. doi: 10.1111/aji.12853.
43.    Matole V, et al. A brief review on herbal medicines. Res J Pharmacogn Phytochem. 2021; 13(2): 101-2. doi: 10.52711/0975-4385.2021.00016
44.    Singh S, Bharti PK, Shivhare Y, Singh P. Herbal options in pregnancy: A review. Res J Sci Tech. 2011; 3(3): 127-128.

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