Author(s): Tri D. Widyaningsih, Rinten A. Sari, Widya D. Rukmi, Syavira A. Dahniar


DOI: 10.52711/0974-360X.2022.00751   

Address: Tri D. Widyaningsih*, Rinten A. Sari, Widya D. Rukmi , Syavira A. Dahniar
Department of Food Science and Technology, Faculty of Agricultural Technology, Brawijaya University, Malang, Indonesia.
*Corresponding Author

Published In:   Volume - 15,      Issue - 10,     Year - 2022

Giving herbal supplements made from moringa leaf extract 70%, fragrant pandanus 20% and red ginger 10% (KPJ) can lower uric acid levels of rat hyperuricemia significantly. To know the industrial scale production process and its influence on the chemical parameters of capsules extract formula KPJ extract conducted analysis of moisture content, total phenols, flavonoids and antioxidant activity (DPPH, FRAP and ABTS). Meanwhile, knowing the effect of herbal supplements formula moringa leaf extract, pandan fragrance, and red ginger to hyperuricemia sufferers, clinical trials conducted phase I on 40 people with hyperuricemia who consume herbal supplements formula moringa leaf extract, pandan leafe, and red ginger for 14 days. Furthermore, checks on uric acid levels, kidney function, and liver function before and after the intervention of herbal supplements formula moringa leaf extract, pandanus, and red ginger to find out the effectiveness of clinical trials phase I. The results showed that the best supplements are produced from the formula of thick extract KPJ as much as 60% and filler ingredients as much as 40%. The supplement has a water content of 5.49%, phenol 10.67mg GAE/g, flavonoids 4.37mg QE/g, DPPH 7.19mg TE/g, FRAP 12.82mg TE/g, ABTS 29.04mg TE/g, IC50 545.57ppm. Phase II research is a clinical trial phase I. This stage aims to determine the influence of KPJ supplements on uric acid levels, kidney function, and liver function before and after intervention and perception of hyperuricemia sufferers after intervention The perception results of hyperuricemia sufferers showed 65% of sufferers experienced a decrease in uric acid levels and 57.5% of sufferers were satisfied with the benefits and results of KPJ herbal supplements in lowering uric acid levels

Cite this article:
Tri D. Widyaningsih, Rinten A. Sari, Widya D. Rukmi , Syavira A. Dahniar. The Effect of Moringa Leaf (Moringa oleifera Lam.), Pandan Wangi (Pandanus amaryllifolius Roxb.) and Red Ginger (Zingiber officinale Rosc.Var) Extract Supplement Intervention on Uric Acid, Kidney, Liver Function and Perceptions of Hyperuricemia patients on health benefit. Research Journal of Pharmacy and Technology2022; 15(10):4477-4. doi: 10.52711/0974-360X.2022.00751

Tri D. Widyaningsih, Rinten A. Sari, Widya D. Rukmi , Syavira A. Dahniar. The Effect of Moringa Leaf (Moringa oleifera Lam.), Pandan Wangi (Pandanus amaryllifolius Roxb.) and Red Ginger (Zingiber officinale Rosc.Var) Extract Supplement Intervention on Uric Acid, Kidney, Liver Function and Perceptions of Hyperuricemia patients on health benefit. Research Journal of Pharmacy and Technology2022; 15(10):4477-4. doi: 10.52711/0974-360X.2022.00751   Available on:

1.    Li L. Zhang Y. Zeng C. Update on the epidemiology, genetics, and therapeutic options of hyperuricemia. 2020;12(7):3167-3181.
3.    Rivera-paredez B. Mac L. Fernandez-lopez JC. et al. Influence of Genetic and Non-Genetic Risk Factors for Serum Uric Acid Levels and Hyperuricemia in Mexicans. 2019;1014290:1-19.
4.    Vedder D. Walrabenstein. Heslinga M. Vries R De. Dietary interventions for gout and effect on cardiovascular risk factors : A systematic review. Nutrients. 2019;11(2955):1-19.
5.    Ramesh S. Vanita G. Evaluation of anti-hyperuricemic activity of the alcoholic extract of dried capparis moonii wight fruits in wistar rats. Res J Pharm Technol. 2021;14(6):3173-3178. doi:10.5958/0974-360X
6.    Huang Q. Yu J. Zhang X. Liu S. Ge Y. Association of the serum uric acid level with liver histology in biopsy-proven non-alcoholic fatty liver disease. Biomed Reports. 2016;5(2):188-192. doi:10.3892/br.2016.698
7.    Roddy E. Choi H. Epidemiology of gout. Natl Intitutes Heal. 2015;40(2):155-175. doi:10.1016/j.rdc.2014.01.001
8.    Robinson PC. Gout – An update of aetiology, genetics, co-morbidities and management. Maturitas. 2018;118:67-73. doi:10.1016/j.maturitas.2018.10.012
9.    Wang H. Zhang H. Sun L. Guo W. Roles of hyperuricemia in metabolic syndrome and cardiac-kidney-vascular system diseases. Am J Transl Res. 2018;10(9):2749-2763.
10.    Ni Q. Lu X. Chen C. Du H. Zhang R. Risk factors for the development of hyperuricemia. Medicine (Baltimore). 2019;98(42):1-6.
11.    Sato Y. Feig DI. Stack AG. et al. The case for uric acid-lowering treatment in patients with hyperuricaemia and CKD. Nat Rev Nephrol. 2019;15(12):767-775. doi:10.1038/s41581-019-0174-z
12.    Chaudhary NS. Jr SLB. Saag KG. et al. Severity of hypertension mediates the association of hyperuricemia with stroke in the Regards Case Cohort Study. Am Hear Assoc. Published online 2019:1-11. doi:10.1161/HYPERTENSIONAHA.119.13580
13.    Steiger S. Ma Q. Anders HJ. The case for evidence-based medicine for the association between hyperuricaemia and CKD. Nat Rev Nephrol. 2020;16(7):422. doi:10.1038/s41581-020-0288-3
14.    Burns CM. Wortmann RL. Latest evidence on gout management: What the clinician needs to know. Ther Adv Chronic Dis. 2012;3(6):271-286. doi:10.1177/2040622312462056
15.    Hendriani R. Sukandar EY. In vitro evaluation of xanthine oxidase inhibitory activity of selected medicinal plants. Int J Pharm Clin Res. 2016;8(4):235-238.
16.    Ragab G. Elshahaly M. Bardin T. Gout: An old disease in new perspective – A review. J Adv Res. 2017;8(5):495-511. doi:10.1016/j.jare.2017.04.008
17.    Sahu P. Sahu K. Dubey RD. Chatterjee S. Chatterjee T. Herbal Antioxidants: A Review. Res J Pharmacogn Phytochem. 2010;2(6):4427-4434.
18.    Alrawaiq N. A. Review of flavonoid quercetin: Metabolism . Bioactivity and Antioxidant Properties. Int J PharmTech Res. 2019;6(3):933-941.
19.    Widyaningsih TD. Rachmawati M. Prabawati E. Antihyperuricemia of functional products intergrated food therapy formula of moringa leaves, Pandan wangi and red ginger on wistar rat induced by potassium oxonate. Vol 2.; 2017.
20.    Mohamed Isa SSP. Ablat A. Mohamad J. The antioxidant and xanthine oxidase inhibitory activity of plumeria rubra flowers. Molecules. 2018;23(2). doi:10.3390/molecules23020400
21.    Erkmen O. Bozoglu TF. Food Microbiology Principles into Practice.; 2016. doi:10.1002/9781119237860.ch30
22.    Hasanah Q. Andrianto D. Faridah N. Aktivitas antioksidan dan antihiperkolesterolemia in vitro dari campuran ekstrak angkak dan bekatul. 2018;29(2):145-154. doi:10.6066/jtip.2018.29.2.145
23.    Tiwari P. Phenolics and flavonoids and antioxidant potential of balarishta prepared by traditional and modern methods. Asian J Pharm Ana. 2014;4(1):5-10.
24.    Sayuti K. Yenrina R. Antioksidan, alami dan sintetik.; 2015.
25.    Narasimhan R. Sathiyamoorthy. Phytochemical screening and antioxidant studies in the pulp extracts of cucurbita maxima. Asian J Pharm Ana. 2016;6(1):04-04.
26.    Selvakumar K. Madhan R. Srinivasan G. Bakar V. Antioxidants assays in pharmacological research. Asian J Pharm Tech. 2011;1(4):99-103.
27.    Chu YH. Chang CL. Hsu HF. Flavonoid content of several vegetables and their antioxidant activity. J Sci Food Agric. 2000;80(5):561-566.
28.    Karadag A. Ozcelik B. Saner S. Review of methods to determine antioxidant capacities. 2009;(November 2008):41-60. doi:10.1007/s12161-008-9067-7
29.    Prior RL. Wu X. Identification and characterization of anthocyanins by high-performance liquid chromatography − electrospray ionization − tandem mass spectrometry in common foods in the united states: Vegetables, nuts, and grains. J Agric Food Chem. 2005;53:3101-3113.
30.    Huyut Z. Beydemir F. Gülçin E. Antioxidant and antiradical properties of selected flavonoids and phenolic compounds. 2017;2017.
31.    Sushil DP. Masood A. Aher P. Pavin B. Yardi S. Synthesis and evaluation of novel flavonoid derivatives for antibacterial activity. Asian JPharm Res. 2016;6(1):27-30.
32.    Iraz M. Dose. Dependent effects of caffeic acid phenethyl ester on heart rate and blood pressure in rats. Eur J Gen Med. 2005;2(2):69-75.
33.    Septian BA. Widyaningsih TD. The role of black jelly grass drinks (Mesona palustris Bl .) For high blood pressure reduction: a Review. J Pangan dan Agroindustri. 2014;2(3):198-202.
34.    Kusmiyati A. Kadar asam urat serum dan urin tikus putih hiperurikemia setelah pemberian jus kentang (Solanum tuberosum L.). Thesis. Departement Biology. Universitas Negeri Solo. Surakarta
35.    Vermerris W. Nicholson R. Phenolic compound biochemistry.
36.    Dahal A. Mulukuri S. Flavonoids in kidney protection. World J Pharm Sci. 2015;4:362-382.
37.    Buvana C. Sumathy A. Sukumar M. In silico identification of potential xanthine oxidase inhibitors for the treatment of gout and cardiovascular disease. Asian J Res Chem. 2013;6(11):1049-1053.
38.    Aryadi. Uji ekstrak methanol daun kepel (Stelechocarpus burahol (BI.)Hook.F.&Th.) terhadap aktivitas enzim xantin oksidase secara in vitro.Thesis. Departemen Farmasi. Universitas Gadjah Mada. Yogyakarta
39.    Permana P. EB. Sumarni S. Nisa FZ. Faktor yang berhubungan dengan preferensi konsumen street food pada mahasiswa Universitas Gadjah Mada. J Gizi dan Diet Indones (Indonesian J Nutr Diet. 2016;3(3):131. doi:10.21927/ijnd.2015.3(3).131-138
40.    Huda AK. Persepsi direktur dan tenaga medis terhadap layanan bimbingan rohani islam dan relevansinya dalam meningkatkan kualitas layanan di RSUD Ambarawa. Published online 2017.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

56th percentile
Powered by  Scopus

SCImago Journal & Country Rank

Recent Articles


Not Available