Author(s): Chrismawan Ardianto, Ardian Lestari Judoko, Mirza Aprilia, Dinda Monika Nusantara Ratri, Toetik Ariyani, Mahardian Rahmadi, Junaidi Khotib


DOI: 10.52711/0974-360X.2022.00800   

Address: Chrismawan Ardianto, Ardian Lestari Judoko, Mirza Aprilia, Dinda Monika Nusantara Ratri, Toetik Ariyani, Mahardian Rahmadi, Junaidi Khotib
Nanizar Zaman Joenoes Building, Kampus C, UNAIR. Jl. Mulyerejo, Surabaya, 60115.
*Corresponding Author

Published In:   Volume - 15,      Issue - 10,     Year - 2022

Chemotherapy-induced peripheral neuropathy (CIPN) is a one of side effect in cancer patients that receive antineoplastic agent, like oxaliplatin. Orexinergic system in the hypothalamus is the one of system that modulate nociceptive and neuropathy. Because there is flavonoids such as resveratrol and andrographolide that may prevent chemotherapy induced peripheral neuropathy, this study analyzed effects of andrographolide and resveratrol treatment on PPOrx and OX1R mRNA expression in hypothalamic oxaliplatin-induced mice. Materials and Methods This study was conducted for 22 days in mice. Mice injected with oxaliplatin followed by andrographolide or resveratrol. Chemotherapy induced peripheral neuropathic pain was assessed based on withdrawal threshold, mRNA PPOrx expression, and mRNA OX1R expression. Results The results showed that intraperitoneal injection of 100mg/kg resveratrol and 20mg/kg andrographolide increased the withdrawal threshold after oxaliplatin induction. Resveratrol administration also increased the relative expression of PPOrx mRNA significantly, but not the OX1R mRNA relative expression. On the other hand, administration of andrographolide did not cause a change in the expression of PPOrx and OX1R in the hypothalamus. Conclusions Intraperitoneal injection of andrographolide and resveratrol reduces the mechanical allodynia response in oxaliplatin-induced mice significantly. The mechanism of andrographolide increases the withdrawal threshold does not via the orexinergic system, but the mechanism of resveratrol via the orexinergic system.

Cite this article:
Chrismawan Ardianto, Ardian Lestari Judoko, Mirza Aprilia, Dinda Monika Nusantara Ratri, Toetik Ariyani, Mahardian Rahmadi, Junaidi Khotib. Effect of Andrographolide and Resveratrol on OX1R and Prepro-orexin mRNA expression in CIPN-induced hypothalamus of mice with oxaliplatin. Research Journal of Pharmacy and Technology 2022; 15(10):4765-1. doi: 10.52711/0974-360X.2022.00800

Chrismawan Ardianto, Ardian Lestari Judoko, Mirza Aprilia, Dinda Monika Nusantara Ratri, Toetik Ariyani, Mahardian Rahmadi, Junaidi Khotib. Effect of Andrographolide and Resveratrol on OX1R and Prepro-orexin mRNA expression in CIPN-induced hypothalamus of mice with oxaliplatin. Research Journal of Pharmacy and Technology 2022; 15(10):4765-1. doi: 10.52711/0974-360X.2022.00800   Available on:

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