Author(s): Cheemalapati Venkata Narasimhaji, A K Mangal, Rekha Prabu, Ilavrasan R, Narayanam Srikanth

Email(s): ,

DOI: 10.52711/0974-360X.2022.00835   

Address: Cheemalapati Venkata Narasimhaji1*, A K Mangal2, Rekha Prabu1, Ilavrasan R1, Narayanam Srikanth2
1Captain Srinivasa Murthy Central Ayurveda Research Institute (CSMCARI), CCRAS, Chennai.
2Central Council for Research in Ayurvedic Sciences (CCRAS), New Delhi.
*Corresponding Author

Published In:   Volume - 15,      Issue - 11,     Year - 2022

Efficacy of the medicinal plants depends on the nature of primary and secondary metabolites present and assay of metabolites in raw botanical parts used for the drugs. Quantity and nature of metabolites that are presented are correlated with the growth of the plants, herbs and the season in which they are collected. Hence, it is important for a search of the best time and season for collection, based on the part used of medicinal plants, the assay of the secondary metabolites present and other morphological features for assess the maturity etc., In this study, it is aimed to evaluate the seasonal variation in terms of assay of wedelolactone through RP-HPLC and validate the best procurement time for the whole plant of Eclipta prostrata L. besides botanical verification and pharmacognostic authentication. The developed method was validated, results of analytical findings were summarized by all three triplicate tests for the three different solvent extracts of whole plant material of Bhringraj for all collected six seasons and found more abundance of wedelolactone in the range of 0.146-0.209 % w/w in Sharad ritu (Sep-Oct)/ Autumn season.

Cite this article:
Cheemalapati Venkata Narasimhaji, A K Mangal, Rekha Prabu, Ilavrasan R, Narayanam Srikanth. Evaluation of Seasonal Variation and Procurement Time for Eclipta prostrata (L.) L.C.. Research Journal of Pharmacy and Technology. 2022; 15(11):4967-3. doi: 10.52711/0974-360X.2022.00835

Cheemalapati Venkata Narasimhaji, A K Mangal, Rekha Prabu, Ilavrasan R, Narayanam Srikanth. Evaluation of Seasonal Variation and Procurement Time for Eclipta prostrata (L.) L.C.. Research Journal of Pharmacy and Technology. 2022; 15(11):4967-3. doi: 10.52711/0974-360X.2022.00835   Available on:

1.    C. P. Khare. Indian Medicinal Plants. An Illustrated Dictionary, Springer, Berlin, Germany. 2007.
2.    Anonymous. The Wealth of India CSIR. New Delhi. 1952.
3.    Commentary of Chakrapanidatta, Ed. By Yadavji Trikamji Acharya, Chaukhambha Sanskrit Sansthan, Varanasi, Chikitsa sthanam. 1984; 22/115.
4.    Suśruta Saṃhitā of Maharṣi Suśruta with Bhanumati commentary by Chakrapanikdatta, Ed. By Acharya Y.T. and Sharma N.K. Pub. By Pt. Sharma S.S., Registrar, Agra University, Secretary Swami Laxmi Ram Trust. Su.S.U.51; 1939.
5.    Aśtāngahṛdayam of Vāgbhata, Vidyotini Commentary by Kaviraj Atrideva Gupta, Ed. Yadunandana Upadhyaya, Chaukhambha Sanskrit Sansthan, Varanasi, India. 8th Ed, 1982; A.H.Ci 20/8.
6.    Charakasamhita by Agnivesha revised by Charaka and Dridhbala with Ayurveddipika Commentary of Chakrapanidatta, Yadavji Trikamji Acharya, Chaukhambha Sanskrit Sansthan, Varanasi, Chikitsa sthanam.1984; 26/265.
7.    Verma and Flora MP. Published by Director, Botanical Survey of India, Calcatta. 1993: Vol, 1-3.
8.    Thakur M. Roy RK. Dixit VK et al Hair growth promoting activity of Eclipta alba in male albino rats,Arch. Dermatol.Res.2008,300: 357–364. DOI: 10.1007/s00403-008-0860-3.
9.    Fang Y. Deng H et al Anti-inflammatory gallic acid and wedelolactone are G protein-coupled receptor-35 agonists. Pharmacology.2012,89: 211–219. DOI: 10.1159/000337184.
10.    Agrawal, S. Tabassum N et al Hepatoprotective activity of Eclipta Alba Hassk Against paracetamol induced hepatocellular damage in mice. JK Pract. 2004,11:278–280.
11.    Mishra M. Kumar S. Manvar D. Pandey V N et al Identification and evaluation of anti Hepatitis C virus phytochemicals from Eclipta alba.J.Ethnopharmacol. 2012,144: 545–554. DOI: 10.1016/j.jep.2012.09.036.
12.    Tan X. Sun H. Huang H. Jin P. Jia X. Liu W et al Protective effect and mechanism of Ecliptae Herba on cigarette smoke extract-induced cytotoxicity of NHBE cells. ZhongguoZhongYaoZaZhi. 2012,37:2444–2447.
13.    Subhadhirasakul S. Tansakul P. Cheenpracha S. Karalai C. Tewtrakul S et al Anti in flammatory constituents from Eclipta prostrate using RAW 264.7 macrophage cells. Phytother.Res. 25; 2012:1313–1316.
14.    Bhatia V. Srivastava SP. Srivastava AK. Jaiswal N. Tamrakar AK et al Antidiabetic effect of Eclipta alba associated with the inhibition of alpha-glucosidase and aldose reductase. Nat. Prod. Res.2012,26: 2363–2367. DOI: 10.1080/14786419.2012.662648.
15.     Paguigan ND. Chichioco-Hernandez CL et al Phytochemical profile of selected Philippine plants used to treat asthma. Pharmacogn. J. 2010, 2: 198–202.DOI:10.1016/s0975-3575(10)80092-6.
16.    Gairola S. Gaur RD. Painuli R M. Sharma J et al The treatment of jaundice with medicinal plants in indigenous communities of the Sub Himalayan region of Uttarakhand. India J. Ethnopharmacol. 2012,14:262–291. DOI: 10.1016/j.jep.2012.06.034.
17.    Al-Nahain A. Majumder S. Jahan R. Rahmatullah M et al Ethnopharmacological significance of Ecliptaalba (L.) Hassk (Asteraceae). Int. Sch.Res.Notices. 2014:385969.  DOI: 10.1155/2014/385969.
18.    You X Y. Xue Q. Fang Y. Liu QY.Zhao C. Zhang  CF et al  Preventive effects of Ecliptae Herba extract and its component ecliptasaponin A on bleomycin-induced pulmonary fibrosis in mice. J. Ethnopharmacol. 2015, 175: 172–180. DOI: 10.1016/j.jep.2015.08.034.
19.    Ding S. Hou X. Yuan J. Tan X. Chen J. Yang N. Z Dong et al Wedelolactone protects human bronchial epithelial cell injury against cigarette smoke extract induce doxidant stress and inflammation responses through Nrf2 pathway. Int. Immunopharmacol. 2015, 29: 648–655. DOI: 10.1016/j.intimp.2015.09.015.
20.    Xia YZ. Chen J. Cao Y. Xu CS. Li RM. Pan Y. Xiao Chen et al Wedelolactone exhibits anti-fibrotic effects on human hepatic stellate cell line LX-2. Eur. J. Pharmacol. 2013, 714: 105–111. DOI: 10.1016/j.ejphar.2013.06.012.
21.    Ragya E. Santhosh A. Suma G. Swapna K. Ramesh CH. Venkateshwarlu G et al Eclipta alba -An Important Medicinal Plant: A Review of Its Folklore Medicine and Traditional Uses. Asian J. Pharm. Tech. 2014, 4; 3: 163-164.  
22.    Geetha P. Jesuraja A. Vinoth KM. Kousalya M  et al In-Vitro Study of Anthelmintic Activity of Eclipta prostrata (L) various Extracts. Research J. Pharm. and Tech, 2017 January 10,1:58-60. DOI: 10.5958/0974-360X.2017.00014.2.
23.    Patel JK.  Kulkarni PS.  Patel MU.  Bhavsar CJ.  Patel JA. Patil P et al In Vitro Anthelmintic Activity of Various Herbal Plants Extracts against Pheritima posthuma. Research J. Pharmacognosy and Phytochemistry. 2010, 2; 3: 234-237.
24.    Ravishankar K. Vijaya Nandini. Palaksha MN et al Evaluation of the Immuno modulatory Effect of Eclipta prostrata whole Plant Extract on Albino Rats. Research Journal of Pharmacology and Pharmacodynamics. 2017 June 9; 2:41-45. DOI: 10.5958/2321-5836.2017.00008.8.
25.    Prince Thomas. Himanshu Joshi.  Prasannashama K. Ullas D’Souza. Nimmy Chacko.  Bharath Raj KC. Rajesh KS. Gururaja MP et al Ameliorative Potential of Eclipta alba on Gentamycin Induced Renal Toxicity in Rats. Research J. Pharm. and Tech. 2019 January 12; 1:192-196. DOI: 10.5958/0974-360X.2019.00035.0.
26.    Gupta V. Gupta A. Jayalakshmi S. Agarwal A et al Phytochemical Evaluation of Extracts of Stem of Eclipta alba (Bhringaraja). Research J. Pharmacology and Pharmacodynamics. 2009, 1; 3:134-139.
27.    Shashidhara S. Vivek KR. Mithun NM et al Eclipta alba (L.) A review on its phytochemical and pharmacological profile. Pharmacology online. 2011, 1; 345–357.
28.    Upadhyay RK. Pandey MB. Jha RN. Pandey VB et al Eclalbatin, a triterpene saponin from Eclipta alba. Journal of Asian Natural Products Research. 2001, 3; 3: 213–217.
29.    Chen Y Y. Di XH. Liu M. Zhang M et al Isolation and identification of ecliptasaponin D from Eclipta alba(L.) hassk. Yao Xue Xue Bao. 1997, 32; 8: 633–634.
30.    Chen Y. Zhang M et al Chemical constituents of Eclipta alba (L.) Hassk. Zhongguo Zhong Yao ZaZhi. 1996, 21; 8: 480– 510.
31.    Qing Chen. Xuemin Gao. Hua Song.  Xuan Zhu. Xiaoxue Wu et al Development and Validation of an Ultra-Performance Liquid Chromatography Method for the Determination of Wedelolactone in Rat Plasma and its Application in a Pharmacokinetic Study. Molecules. 2019, 24;4: 762. DOI:10.3390/molecules24040762.
32.    Cheemalapati VN. Mangal AK. Rekha P. Srikanth N. Sharma N et al Vasa (Justicia adhatoda L.) Leaves Best Procurement Time with Special Reference to Assay of Vasicine through HPLC and its Comparative Pharmacognosy. J Drug Res Ayurvedic Sci. 2019, 4; 4: 168-173. DOI:10.5005/jdras-10059-0082.
33.    Cheemalapati VN. Mangal AK. Srikanth N. Murthy SN. Sharma N et al Evaluation of the Best Procurement Time for Ashwagandha [Withania somnifera (L.) Dunal] Root by Liquid Chromatographic and Pharmacognostical Studies. J Drug Res Ayurvedic Sci. 2018, 3; 4: 206-213. DOI:10.5005/jdras-10059-0053.
34.    Cheemalapati VN. Mangal AK. Murthy SN. Pant P. Srikanth N. Dhiman KS. Rekha P. Ilavarsan R. Sharma N et al Validation of the Best Procurement Season of Arjuna Bark [Terminalia arjuna (Roxb. ex DC.) Wight & Arn.] by Comparative HPLC and Pharmacognostical Studies. J Drug Res Ayurvedic Sci. 2018, 3; 3:165-172. DOI:10.5005/jp-journals-10059-0050.
35.    Cheemalapati VN. Mangal AK.  Rekha P.  Srikanth N.  Murthy SN. Sharma N et al Evaluation of Procurement time of Kalmegh [Andrographis paniculata (Burm.f.) Nees] through HPLC,Macro and Microscopic studies. J Drug Res Ayurvedic Sci. 2019, 4; 2: 47-54. DOI:10.5005/jdras-10059-0075.
36.    Chowdhury SR. Shihan MH. Maleque M et al Development and Validation of a Simple RP-HPLC Method for Determination of Caffeine in Pharmaceutical Dosage Forms. Asian J. Pharm. Ana. 2012, 2; 1: 01-04.
37.    Yada JS. Swati R. M Gandhi M. Gupta A et al Method Development and Hydrolytic Degradation Study of Doxofylline by RP-HPLC and LC-MS/MS. Asian J. Pharm. Ana. 2011, 1; 1: 14-18.
38.    Sharada N. Venkateswarlu G. Sravan PM. Praveena K M. Madhu CS. Manoranjan S et al  A New Analytical Method Development and Validation for Estimation of Ciprofloxacin in Bulk and Pharmaceutical Dosage Form. Asian J. Pharm. Ana. 2012, 2; 4: 116-117.
39.    Mohammad S. Parvin NM. Islam ASM. Ahsanul HMd et al Validated RP-HPLC Method for Estimation of Ranitidine Hydrochloride, Domperidone and Naproxen in Solid Dosage Form. Asian J. Pharm. Ana. 2011, 1; 3: 59-63.

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