Author(s): Rupali Deshpande, Chandrashekar K.S., Vasudev R Pai, Venkatesh Kamath, Aswatha Ram H.N.


DOI: 10.52711/0974-360X.2022.00861   

Address: Rupali Deshpande1, Chandrashekar K.S.1, Vasudev R Pai1, Venkatesh Kamath2, Aswatha Ram H.N.1*
1Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India.
2Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India.
*Corresponding Author

Published In:   Volume - 15,      Issue - 11,     Year - 2022

Rajanyadi churna (RC) is a polyherbal traditional formulation prescribed in Ayurveda for treating diarrhea, gastric disorders, asthma, fever, anemia and jaundice. In the current experiment, various in vitro models of antioxidant activity, and antibacterial activitity against Escherichia coli of Rajanyadi churna alcoholic extract (RCAL) and Rajanyadi churna aqueous extract (RCAQ) are presented. In vitro antioxidant potential was determined for both the extracts in various models like ABTS, Nitric Oxide, DPPH, Superoxide and Hydrogen peroxide scavenging assays. The minimum inhibitory concentration (MIC) of RC extracts against Escherichia coli, Gram negative bacteria were determined using the micro-dilution technique (ATCC 11775). Our study showed a remarkable improvement in the antioxidant activity of RCAL and RCAQ in all the in vitro models. The percentage of inhibition for RCAL and RCAQ were found to be 31% and 25% respectively against E. coli. From this experimental study, we conclude that Rajanyadi churna extracts showed good antioxidant activity and antibacterial activity against E. coli.

Cite this article:
Rupali Deshpande, Chandrashekar K.S., Vasudev R Pai, Venkatesh Kamath, Aswatha Ram H.N.. In vitro Antioxidant Potential and Anti-bacterial Activities of Rajanyadi Churna Extracts Against Escherichia coli. Research Journal of Pharmacy and Technology. 2022; 15(11):5120-5. doi: 10.52711/0974-360X.2022.00861

Rupali Deshpande, Chandrashekar K.S., Vasudev R Pai, Venkatesh Kamath, Aswatha Ram H.N.. In vitro Antioxidant Potential and Anti-bacterial Activities of Rajanyadi Churna Extracts Against Escherichia coli. Research Journal of Pharmacy and Technology. 2022; 15(11):5120-5. doi: 10.52711/0974-360X.2022.00861   Available on:

1.    Halliwell B, Gutteridge JMC. Free radicals in biology and medicine. 2nd ed. Oxford: Clarendon Press, 1989.
2.    Wickens AP. Ageing and the free radical theory. Respiration Physiology. 2001 Nov 15; 128 (3):379-91. doi: 10.1016/s0034-5687(01)00313-9.2001; 28: 379-91.
3.    Velioglu YS, Mazza G, Gao L, Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. Journal of Agricultural and Food Chemistry. 1998; 46: 4113-17.
4.    Altmann HJ, Grunow W, Mohr U, Richter-Reichhelm HB, Wester PW. Effects of BHA and related phenols on the forestomach of rats. Food and Chemical Toxicology. Oct-Nov 1986; 24(10-11):1183-8. doi: 10.1016/0278-6915(86)90306-6.
5.    Van Esch GJ, Toxicology of tert-butylhydroquinone (TBHQ). Food and Chemical Toxicology. Oct-Nov 1986;24(10-11):1063-5. doi: 10.1016/0278-6915(86)90289-9.
6.    Su L, Yin JJ, Charles D, Zhou K, Moore J, Yu L. Total phenolic contents, chelating capacities, and radical-scavenging properties of black peppercorn, nutmeg, rosehip, cinnamon and oregano leaf. Food Chemistry. 2007; 100: 990-97.
7.    Jayaprakasha GK, Jaganmohan Rao L. Phenolic constituents from lichen Parmotrema stuppeum (Nyl.) Hale and their antioxidant activity. Zeitschrift für Naturforschung. C, Journal of Biosciences. Nov-Dec 2000;55(11-12):1018-22. doi: 10.1515/znc-2000-11-1227.
8.    The Ayurvedic Formulary of India, 2nd ed. Part-I.  Ministry of Health and Family Welfare, Government of India, New Delhi. (2003) 103-115. Available online at
9.    Parks DA, Bulkley GB, Granger DN. Role of oxygen-derived free radicals in digestive tract diseases. Surgery. 1983; 94(3): 415–22.
10.    Editorial Review, Oxidative stress in childhood malnutrition and diarrheal diseases. Journal of Diarrhoeal Diseases Research. 1994; 12(3): 165-72.
11.    Oran B, Atabek E, Karaaslan S, Reisli Y, Gültekin F., Erkul Y. Oxygen free radicals in children with acute rheumatic fever. Cardiology in the Young. 2001; 11(3): 285-88.
12.    Tsan-Zon Liu, King-Teh Lee, Chi-Liang Chern, Jiin-Tsuey Cheng, Arnold Stern, Li-Yu Tsai. Free Radical-Triggered Hepatic Injury of Experimental Obstructive Jaundice of Rats Involves Overproduction of Proinflammatory Cytokines and Enhanced Activation of Nuclear Factor kappa B. Annals of Clinical Laboratory and Science. 2001; 31(4): 383-90.
13.    Rumiantsev AG, Samochatova EV, Afanas'ev IB, Korkina LG, Suslova TB. The role of free oxygen radicals in the pathogenesis of Fanconi's anemia. Ter Arkh. 1989; 61(7):  32-36.
14.    Ryszard Dworski. Oxidant stress in asthma. Thorax. (2000; 55 (Suppl 2): S51–S53.
15.    Mathabe MC, Nikolova RV, Lall N, Nyazema NZ. Antibacterial activities of medicinal plants used for the treatment of diarrhea in Limpopo Province, South Africa. Journal of Ethnopharmacology. 2006 Apr 21;105(1-2):286-93. doi: 10.1016/j.jep.2006.01.029. Epub 2006 Mar 20.
16.    Helander IM, Alakomi HL, Latva-Kala K, Mattila-Sandholm T, Pol I, Smid, EJ, et al. Characterization of the action of selected essential oil components on Gram-negative bacteria. Journal of Agricultural and Food Chemistry. 1998; 46: 3590-95.
17.    Sreejayan N, Rao M.  Free radical scavenging activity of curcuminoids. Arzneimittel-forschung. 1996; 46(2): 169-71.
18.    Re R, Pellegrini N, Proteggente A, Pannala A, Yang M,  Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 1999; 26(9): 1231-37.
19.    Shirwaikar A, Ram HNA, Mohapatra P, Antioxidant and antiulcer activity of aqueous extract of a polyherbal formulation. Indian Journal of Experimental Biology. 2006; 44(6): 474-80.
20.    Sánchez-Moreno C. Review: Methods used to evaluate the free radical scavenging activity in foods and biological systems. Food Science and Technology International. 2002; 8(3):  121-37.
21.    Ruch RJ, Cheng SJ,  Klaunig JE. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis. 1989 Jun;10(6):1003-8. doi: 10.1093/carcin/10.6.1003.
22.    Madikizela B, Ndhlala A, Finnie J, Van Staden J.  Ethnopharmacological study of plants from Pondoland used against diarrhoea. Journal of Ethnopharmacology. 2012 May 7;141(1):61-71. doi: 10.1016/j.jep.2012.01.053. Epub 2012 Feb 7.
23.    Savitree M, Isara P, Nittaya SL, Worapan S. Radical scavenging activity and total phenolic content of medicinal plants used in primary health care. Journal of Pharmaceutical Sciences. 2004; 9: 32-35.
24.    Nile SH, Khobragade CN. Antioxidant activity and flavonoid derivatives of Plumbago zeylanica. Journal of Natural Products.  2010; 3: 130-33.
25.    Sreejayan N, Rao M. Free radical scavenging activity of curcuminoids. Arzneimittel-forschung.  1996; 46(2): 169-71. PMID: 8720307.
26.    Ialenti A, Moncada S, Di-rose, M. Modulation of adjuvant arthritis by endogenous nitric oxide. British Journal of Pharmacology. 1993 Oct;110(2):701-6. doi: 10.1111/j.1476-5381.1993 tb13868.x.
27.    AI-Mamun M, Yamaki K, Masumizu T, Nakai Y, Saito K, Sano H. et al.   Superoxide anion radical scavenging activities of herbs and pastures in northern Japan determined using electron spin resonance spectrometry. International Journal of Biological Sciences. 2007; 3: 349-55. doi: 10.7150/ijbs.3.349.
28.    Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958; 181: 1199-200.
29.    Van Wijk R, Van Wijk EP, Wiegant FA, Ives J. Free radicals and low-level photon emission in human pathogenesis: State of the art. Indian Journal of Experimental Biology.   2008; 46: 273-309. PMID: 18697612.
30.    Rajamanikandan S, Sindhu T, Durgapriya D, Sophia D, Ragavendran P, Gopalakrishnan VK, Radical Scavenging and Antioxidant Activity of Ethanolic Extract of Mollugo nudicaulis by In vitro Assays. Indian Journal of Pharmaceutical Education and Research.  2011; 45(4): 310-16.
31.    Fennell CW, Lindsey KL, McGaw LJ, Sparg SG, Stafford GI, Elgorashi EE, et al.  Assessing African medicinal plants for efficacy and safety: pharmacological screening and toxicology. Journal of Ethnopharmacology. 2004 Oct;94(2-3):205-17. doi: 10.1016/j.jep.2004.05.012.
32.    Voravuthikunchai S, Lortheeranuwat A, Jeeju W, Sririrak T, Phongpaichit S, Supawita T. Effective medicinal plants against enterohaemorrhagic Escherichia coli O157:H7.   Journal of Ethnopharmacology. 2004 Sep;94(1):49-54. doi: 10.1016/j.jep.2004.03.036.
33.    Pereira JA, Pereira APG, Ferreira ICFR, Valentão P, Andrade PB, Seabra, R, et al.  Table olives from Portugal: phenolic compounds, antioxidant potential and antimicrobial activity. Journal of Agricultural and Food Chemistry. 2006 Nov 1;54(22):8425-31. doi: 10.1021/jf061769j.
34.    Puupponen-Pimiä R, Nohynek L, Meier C, Kähkönen M, Heinonen, M, Hopia A, et al.  Antimicrobial properties of phenolic compounds from berries. Journal of Applied   Microbiology. 2001 Apr;90(4):494-507. doi: 10.1046/j.1365-2672.2001.01271x.
35.    Bravo L, Polyphenols: chemistry, dietary sources, metabolism and nutritional significance. Nutrition Reviews. 1998 Nov;56(11):317-33. doi: 10.1111/j.1753-4887.1998.tb 01670.x.

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