Author(s): Puneet Sudan, Monika Sharma


DOI: 10.52711/0974-360X.2022.00824   

Address: Puneet Sudan1*, Monika Sharma2
1Associate Professor, Regional institute of Cooperative management-Chandigarh. (Mohali) 140307.
2Research Scholar UIPS, Chandigarh University, Gharuan (Mohali) and Chandigarh College of Pharmacy, Landran (Mohali).
*Corresponding Author

Published In:   Volume - 15,      Issue - 11,     Year - 2022

Ocimum gratissimum is a famous herb generally known as Ram Tulsi or Camphor basil. It is a small to mid size herbal plant belonging to Lamiaceae family. The plant usually grows upto height of 2 feet, morphology of leaves revealed that it has cloves-like flavour and odour that makes it an important herb in many cuisines. Ram Tulsi is a vital herb with enormous benefits. The main aim behind this research was to evaluate the antifungal activity of extracts of dried powdered leaves of Ocimum gratissimum in different solvents on the basis of polarity by means of paper disc diffusion method in concentration of 25 µml, 50 µml and 100 µml against Microsporum gypseum. Clotrimazole was selected as a standard drug for comparison. From the results obtained with this research, it was observed that Ocimum gratissimum is an effective candidate to treat dermatophytic infections caused by Microsporum gypseum. The Ocimum gratissimum leaves extracts in ethanol revealed the maximum zone of inhibition of 17.320+ 0.85mm and 39.486% of mycelial inhibition against dermatophytic fungal strain with concentration of 100 µml. In addition to that, extracts made from other solvents also showed antidermatophytic activity but in lesser proportion than the ethanol extracts. It was found that ethanol extract of Ocimum gratissimum is best effective against tested strain. Present investigation of antifungal activity of Ocimum gratissimum extracts has proved it to be a vital candidate, predominantly in removal of dermatophytic infection caused by a particular fungal strain.

Cite this article:
Puneet Sudan, Monika Sharma. Exploration of antifungal potential of extracts of leaves of Ocimum gratissimum against Microsporum gypseum. Research Journal of Pharmacy and Technology. 2022; 15(11):4908-2. doi: 10.52711/0974-360X.2022.00824

Puneet Sudan, Monika Sharma. Exploration of antifungal potential of extracts of leaves of Ocimum gratissimum against Microsporum gypseum. Research Journal of Pharmacy and Technology. 2022; 15(11):4908-2. doi: 10.52711/0974-360X.2022.00824   Available on:

1.    Saha D. Paul S. In Vitro screening of Antifungal Activity of Methanol Extract of Alpinia conchigera Griff against some pathogenic species of Fungi. Asian Journal of Pharmacy and Technology. 2012; 2(2): 44-46.
2.    Shah R R. Patil P J. Adnaik P R. Adnaik R S. Biochemical profiling of Antifungal soap activity of betel leaf (Piper betle L.) extract and Garlic oil by In vitro method. Asian Journal of Pharmaceutical Research. 2020; 10(4):260-262. doi: 10.5958/2231-5691.2020.00045.3
3.    Karuppiah P. Rajaram S. Antibacterial effect of Allium sativum cloves and Zingiber officinale rhizomes against multiple-drug resistant clinical pathogens. Asian Pacific Journal of Tropical Biomedicine. 2012; 2(8):597–601. doi:10.1016/S2221-1691(12)60104-X
4.    Senthilkumar G. Madhanraj P. Panneerselvam A. Studies on the compounds and its Antifungal Potentiality of Fungi Isolated from Paddy Field Soils of Jenbagapuram village Thanjavur District and South India. Asian journal of Pharmaceutical Research. 2011; 1(1): 19-21.
5.    Talebi S. Sabokbar A. Riazipour M. Saffari M. Comparison of the in vitro Effect of Chemical and Herbal Mouthwashes on Candida albicans. Jundishapur Journal of Microbiology. 2014; 7(12): e12563. doi: 10.5812/jjm.12563
6.    Kimm D. Ahn M. Jung J. Kwon S. Jipark E. Koo H K. Woo M J. Perspectives on the market Globalization of Korean herbal manufacturers: A company - Based survey. Evidence-Based Complementary and Alternative Medicine. 2015; 10(1):1–12.
7.    Malik T. Roy P. Abdulsalam F. Pandey D. Bhattacharjee A. Eruvaram N. Evaluation of the antioxidant, antibacterial, and antidiabetic potential of two traditional medicinal plants of India: Swertia cordata and Swertia chirayita. Pharmacognosy Research. 2015; 7(1): 57-62.  doi:10.4103/0974-8490.157997
8.    Laurence BH. Labiatae oils-mother nature's chemical factory. XIth Initial Congress Essent. Oils, Fragrances and Flavours. New Delhi; 1989.
9.    Svendsen A B. Scheffer J J C. Isolation and analysis of essential oils. In: Verghese J. Essential–Oils. Synthite, Kolenchery, India. 1986.
10.    Robertson S. Narayanan N. Pharmacognostical and Antimicrobial Studies of the Stem Barks of Prosopis cineraria (L) Druce. Research Journal of Pharmacognosy and Phytochemistry. 2009; 1(3):  227-231.
11.    Kumari S. Mazumder A. Mazumder R. Pahwa S. In Vitro Anti-Fungal Activity of the Methanolic Extract of the Leaves Lagerstroemia parviflora (Roxb.). Research Journal of Pharmacognosy and Phytochemistry. 2010; 2(3): 249-251.
12.    Koba K. Sanda K. Guyon C. Raynaud C. Millet J. Chaumont J.P  et al. Chemical composition and in vitro cytotoxic activity of essential oils from two tropical Lamiaceae: Aeollanthus pubescens Benth and Ocimum gratissimum L. Journal of  Essential Oil Bearing Plants 2007;10: 60-69.
13.    Nakamura C V. Ueda-Nakamura T. Bando E. Melo AFN. Cortez DAG. Dias F  et al. Antibacterial activity of Ocimum gratissimum L. essential oil. Memdo Inst Oswaldo Cruz. 1999; 94: 675-678.
14.    Matasyoh LG. Matasyoh JC. Wachira FN. Kinyua MG. Thairu MAW. Mukiama TK. Antimicrobial activity of essential oils of Ocimum Gratissimum L. from different populations of Kenya. African Journal of Traditional Complementary Alternate Med. 2008; 5: 187-193. doi: 10.4314/ajtcam.v5i2.31272
15.    Katara A. Pradhan CK. Singh P. Singh V. Ali M. Volatile constituents and antimicrobial activity of aerial parts of Ocimum gratissimum Linn. Journal of Essential Oil Bearing Plants. 2013; 16: 283-288.
16.    Meena A K. Meena J. Jadhav A. Padhi M M. A review on Hiptage benghalensis (Madhavilata) used as an Ayurvedic drug. Asian Journal of Pharmacy and Technology 2014; 4(1):  28-31.
17.    Pradeepa M. Kalidas V. Geetha N. Qualitative and quantitative phytochemical analysis and bactericidal activity of Pelargonium graveolens L’Her. International Journal of Applied Pharmacy. 2016; 8: 7-11.
18.    Dubey N K. Tiwari T N. Mandin D. Andriamboavonjy H. Chaumont Jean-Pierre. Antifungal properties of Ocimum gratissimum essential oil (ethyl cinnamate chemotype). Fitoterapia. 2000; 71: 567–569. doi: 10.1016/s0367-326x(00)00206-9
19.    Narkar P. Savanta V Raut. Study of Anti biofilm Activity of Methanolic Extract and Oil of Cymbopogon citratus and its other Applications. Research Journal of Pharmacognosy & Phytochemistry. 2014; 6(4): 160-169.
20.    Nwosu M O. Okafor J I. Preliminary studies of the antifungal activities of some medicinal plants against basidiobolus and some other pathogenic fungi. Mycoses. 1995; 38: 191-5. doi: 10.1111/j.1439-0507.1995.tb00048.x
21.    Fokou J B H. Dongmo P M J. Boyom F F. Menkem EZ. Bakargna-Via I. Tsague IFK et al. Antioxidant and antifungal activities of the essential oils of Ocimum gratissimum from Yaoundé and Dschang (Cameroon). Journal of Pharmacy and Pharmacology. 2014; 2: 257-68.
22.    Lemos JdeA. Passos XS. Fernandes OdeFL. de Paula JR. Ferri PH. Souza LKH  et al. Antifungal activity from Ocimum gratissimum L. towards Cryptococcus neoformans. Mem Inst Oswaldo Cruz 2005; 100: 55-58. DOI: 10.1590/s0074-02762005000100011
23.    Silva MR. Oliveira JGJr. Fernandes OF. Passos XS. Costa CR. Souza LK. et al. Antifungal activity of Ocimum gratissimum towards dermatophytes. Mycoses. 2005; 48: 172-175. DOI: 10.1111/j.1439-0507.2005.01100.x
24.    Sudan P. Goswami M.  Singh J. Antifungal potential of Fenugreek Seeds (Trigonella foenum-graecum) Crude Extracts against Microsporum gypseum. International Journal of Research in Pharmaceutical Sciences. 2020; 11(1), 646-649.
25.    Premlata Singariya. Padma Kumar. Krishan Kumar Mourya. In-Vitro Antimicrobial Potency and Pharmacological Study of Flower extracts in different polar solvents of Withania somnifera. Asian Journal of Research in Chemistry. 2012; 5(3): 409-413.
26.    Joshi RK. Chemical composition, in vitro antimicrobial and antioxidant activities of the essential oils of Ocimum gratissimum, O. Sanctum and their major Constituents. Indian Journal of Pharmaceutical Sciences. 2013; 75: 457-62. DOI: 10.4103/0250-474X.119834
27.    Sartoratto A. Machado ALM. Delarmelina C. Figueira GM. Duarte MCT,.Rehder VLG. Composition and antimicrobial activity of essential oils from aromatic plants used in Brazil. Brazilian Journal of Microbiology. 2004; 35: 275-80.
28.    Mith H. Yayi-Ladékan E. Kpoviessi SDS. Bokossa IY. Moudachirou M. Daube G. et al. Chemical composition and antimicrobial activity of essential oils of Ocimum basilicum, Ocimum canum and Ocimum gratissimum in function of harvesting time. J Essential Oil Bearing Plants. 2016; 19: 1413-1425.
29.    Mann A. Phytochemical constituents and antimicrobial and grain protectants activities of clove basil (Ocimum gratissimum L.) grown in Nigeria. International Journal of Plant Research. 2012; 2: 51-8. doi:10.5923/j.plant.20120201.08
30.    Mishra RP. Javed I. In vitro activity of medicinal plants against some bacterial and fungal isolates. Asian Journal of Pharmaceutical and Clinical Research. 2015; 8: 225-230.
31.    Stanley MC. Ifeanyi OE. Chinedum OK. Chinenye ND. The antibacterial activity of leaf extracts of Ocimum gratissimum and Sida acuta. International Journal of Microbiology Research. 2014; 5: 124-129. DOI: 10.5829/idosi.ijmr.2014.5.2.84150
32.    Kpadonou KBG. Ladekan EY. Kpoviessi DS. Gbaguidi F. Yehouenou B. Quetin-Leclercq J. et al. Chemical variation of essential oil constituents of Ocimum gratissimum L. from Benin and impact on antimicrobial properties and toxicity against Artemia salina leach. Chem Biodiversity. 2012; 9:139-50. DOI: 10.1002/cbdv.201100194
33.    Singh P. Jayaramaiah RH. Agawane SB. Vannuruswamy G. Korwar AM. Anand A. et al. Potential dual role of eugenol in inhibiting advanced glycation end products in diabetes: proteomic and mechanistic insights. Scientific Reports. 2016; 6: 18798. DOI: 10.1038/srep18798
34.    Marchese A. Ramona B. Erika C. Ilkay EO. Maria D. Seyed FN. et al. Antimicrobial activity of eugenol and essential oils containing eugenol: a mechanistic viewpoint. Critical Review Microbiology. 2017; 43: 668-89.
35.    Nguefack J. Budde BB. Jakobsen M. Five essential oils from aromatic plants of Cameroon: their antibacterial activity and ability to permeabilize the cytoplasmic membrane of Listeria innocua examined by flow cytometry. Letters in Applied Microbiology. 2004; 39: 395-400.
36.    Sonu Jacob. Anil John J. Leena Thomas. Sabulal B. In vitro pharmacological activity of the whole plant Naregamia alata. Asian Journal of Research in Chemistry. 2012; 5(2):  265-269.
37.    Punasiya R.  Dindorkar G, Pillai S. Antibacterial and Antifungal Activity of Flower extract of Murraya paniculata L. Asian Journal of Research in Pharmaceutical Sciences. 2020; 10(1): 17-20. Doi : 10.5958/2231-5659.2020.00004.1

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

56th percentile
Powered by  Scopus

SCImago Journal & Country Rank

Recent Articles


Not Available