Author(s): Ekhlass N. Ali, Ashwaq A. Kadhem, Huda Zuheir Majeed, Yusra M.B. Muhsin, Hamzia Ali Ajah

Email(s): stkn@uomustansiriyah.edu.iq

DOI: 10.52711/0974-360X.2022.00099   

Address: Ekhlass N. Ali, Ashwaq A. Kadhem, Huda Zuheir Majeed, Yusra M.B. Muhsin, Hamzia Ali Ajah
Biology Department, College of Science, Mustansiriyah University, Iraq, Baghdad.
*Corresponding Author

Published In:   Volume - 15,      Issue - 2,     Year - 2022


ABSTRACT:
Background: The live microorganisms that present in food in addition dietary supplements called Probiotics they have beneficial effect in the human intestine. In the last years, Probiotics has become within treatment options for several disease included immune system. The advent and use of probiotics seem to be increasing day by day all over the world. Objective: The current study included detection of efficacy Pediococcus acidilactici as immunomodulatory in mice that treated with cyclophosphamide. Design: To detect efficacy of Pediococcus acidilactici as immunosuppressive model was used. This model was completed by taking 20 male BALB/C mice with six-weeks old to divide into (5) groups :(G 1) group is the normal control. (G2) group: the group that injected with cyclophosphamide. (G3) group: the immunosuppression plus Pediococcus acidilactici (6×108 CFU/ml). (G4) group: the immunosuppression plus Pediococcus acidilactici (6×104 CFU/ml). last (G5) group : is the group that treated with probiotics. Results: In the current study, thymus and spleen indicators were significantly higher in treated groups than those of (G1) group (NC) with (0.323±0.34) (P<0.05), also Macrophages phagocytosis showed a clear increase in the three treated groups (76.625±108, 72.125±1.65, 87.750±1.32) respectively with significantly (P<0.05), as a compared with (G1) group (NC). Conclusions: The current study reveals the ability of Pediococcus acidilactici (6×109) CFU/ml to accelerate the healing of cyclophosphamide immunosuppressive mice. So, the activity role of probiotic strain as immunomodulator in this study leads us to urge the use it as an alternative treatment for chemical drugs that used against immunosuppression.


Cite this article:
Ekhlass N. Ali, Ashwaq A. Kadhem, Huda Zuheir Majeed, Yusra M.B. Muhsin, Hamzia Ali Ajah. Immunomodulatory effects of Pediococcus acidilactici on BALB/C mice Immunosuppressed by Cyclophosphamide. Research Journal of Pharmacy and Technology. 2022; 15(2):605-0. doi: 10.52711/0974-360X.2022.00099

Cite(Electronic):
Ekhlass N. Ali, Ashwaq A. Kadhem, Huda Zuheir Majeed, Yusra M.B. Muhsin, Hamzia Ali Ajah. Immunomodulatory effects of Pediococcus acidilactici on BALB/C mice Immunosuppressed by Cyclophosphamide. Research Journal of Pharmacy and Technology. 2022; 15(2):605-0. doi: 10.52711/0974-360X.2022.00099   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-2-19


REFERENCES:
1.    Muhsin YMB, Mohammed BB, Shatti ZO. Synergistic effect to Two type of Lactic Acid Bacteria and their Synbiotic effect with Prebiotic Raisin Against Some Pathogenic bacteria. Journal of College of Basic Education. 2017; 23(99): 141-152.
2.    Muhsin YMB, Majeed HZ, Mohammed BB, Mohammed SAA. Lactic Acid Bacteria Bio surfactant Role that isolated from Human Breast Milk in inhibit Eyes Pathogenic Bacteria. Ibn AL-Haith Journal of Pure and Applied Sciences. 2018; 31(2): 31-40.
3.    Venema K, Kok J, Marugg JD, Toonen MY, Ledeboer AM, et al. Functional analysis of the pediocin operon of Pediococcus acidilactici PAC1. 0: PedB is the immunity protein and PedD is the precursor processing enzyme. Molecular Microbiology. 1995; 17(3): 515-522.‏ doi: 10.1111/j.1365-2958.1995.mmi_17030515.x.
4.    Kawashima T, Ikari N, Kouchi T, Kowatari Y, et al. The molecular mechanism for activating IgA production by Pediococcus acidilactici K15 and the clinical impact in a randomized trial. Scientific Reports. 2018; 8(1): 5065.‏ doi: 10.1038/s41598-018-23404-4.
5.    Kim H, Kang SS. Antifungal activities against Candida albicans, of cell-free supernatants obtained from probiotic Pediococcus acidilactici HW01. Archives of Oral Biology. 2019; 99: 113-119.‏ doi: 10.1016/j.archoralbio.2019.01.006.
6.    Jung JY, Shin JS, Lee SG, Rhee YK, et al. Lactobacillus sakei K040706 evokes immunostimulatory effects on macrophages through TLR 2-mediated activation. International immunopharmacology. 2015; 28(1): 88-96. https://doi.org/10.1016/j.intimp.2015.05.037
7.    Shida K, Nanno M, Nagata S. Flexible cytokine production by macrophages and T cells in response to probiotic bacteria: a possible mechanism by which probiotics exert multifunctional immune regulatory activities. Gut Microbes. 2011; 2(2): 109-114. DOI: 10.4161/gmic.2.2.15661 ‏
8.    Ferguson RMW, Merrifield DL, Harper GM, Rawling MD, et al. The effect of Pediococcus acidilactici on the gut microbiota and immune status of on‐growing red tilapia (Oreochromis niloticus). Journal of Applied Microbiology. 2010; 109(3), 851-862. doi: 10.1111/j.1365-2672.2010.04713.x.
9.    Jasim EI, ShawKet DS, Mohsin YMB, Mohammed BB, et al. Comparative study between the effect of the lactic acid bacteria and non against some pathogenic bacteria. International Journal of Advanced Biological and Biomedical Research. 2017; 7(4): 656-660.
10.    Mohsin YMB, Majeed HZ, Hasan AM, Bataah EH. Susceptibility pattern of Pseudomonas aeruginosa producing enzymes against antimicrobial agent cell free supernatant of Lactococcus with Focus on its determining Quantitatively by OD (Enzyme Linked immune sorbent assay). Asian Journal of Pharmaceutical and Clinical Research. 2017; 10(3): 291-294. DOI:10.22159/ajpcr.2017.v10i3.16188
11.    Muhsin YMB, Majeed HZ, Shawkat DS. CFS and Crude Bacteriocin of Lactococcus against Growth and Biofilm formation for some pathogenic bacteria. International Journal of Current Microbiology and Applied Sciences. 2015; 4(7): 35 -42.
12.    Baqer YM, Mohammed BB, Obaid KA, Hlail ZA. CSF of lactobacillus: A natural agent against bacterial contamination of cosmetics tools. International Journal of Advanced Biological and Biomedical Research. 2014; 4(3): 258 – 264.
13.    Jasim EI, Shawket DS, Mohsin YMB, Hasan AM, Mohammed BB. Estimate Bacterial contamination of Iraqi paper Currency and effectiveness of Streptococcus CSF and some Hand Wash to limit it's spread. Indian Journal of Natural Sciences. 2018; 8(49): 14299 – 14307.
14.    Muhsin YMB, Shawket DS, Abd-Alsattar D. Novel probiotic bifidobacterium overcomes synergistic effect of three natural biotic OMNL drug and antibiotic against some UTI pathogens. International Journal of Science and Nature. 2013; 4(3): 456 -462.
15.    Abbas HH, Mohammed SA, Shawket DS, Baqer YM. Effect of Lactobascillus spp. Crude Bacteriocin (CB) and Cell -Free Supernatant (CSF) Against E.coli Growth and Adherence on Vaginal Epithelial cell surface. , International Journal of Advances in Research. 2016; 4(1): 614-620.
16.    Brodsky RA. High-dose Cyclophosphamide for autoimmunity and alloimmunity. Immunology. Research. 2010; 47(1-3): 179-184. doi: 10.1007/s12026-009-8149-y.
17.    Meng Y, Li B, DaJin MZ, Lu J, Huo G. Immunomodulatory activity of Lactobacillus plantarum KLDS1. 0318 in cyclophosphamide-treated mice. Food and Nutrition Research. 2018; 62. doi: 10.29219/fnr.v62.1296.
18.    Xu C, Wang Y, Jin M, Yang X. Preparation, characterization and immunomodulatory activity of selenium-enriched exopoly¬saccharide produced by bacterium Enterobacter cloacae Z0206. Bioresource Technology. 2009; 100(6): 2095–2097. 28. doi: 10.1016/j.biortech.2008.10.037.
19.    Hudson I, Hay FC. Practical Immunology.2nd Ed. Black well scientific publications. Oxford London, Edinburgh, Boston. 1980.
20.    Layoun A, Samba M, Santos MM. Isolation of murine peritoneal macrophages to carry out gene expression analysis upon Toll-like receptors stimulation. Journal of Visualized Experiments. 2015; (98): :e52749. doi: 10.3791/52749.
21.    Ren D, Li C, Qin Y, Yin R, Du S, Liu H, et al. Evaluation of immunomodulatory activity of two potential probiotic Lactobacillus strains by in vivo tests. Anaerobe. 2015; 35(Pt B): 22-27. doi: 10.1016/j.anaerobe.2015.06.008.
22.    Park YJ, Liu G, Tsuruta Y, Lorne E, Abraham E. Participation of the urokinase receptor in neutrophil efferocytosis. Blood. 2009; 114(4): 860-870. https://doi.org/10.1182/blood-2008-12-193524
23.    Villena J, Salva S, Aguero G, Alvarez S. Immunomodulatory and protective effect of probiotic Lactobacillus casei against Candida albicans infection in malnourished mice. Microbial Immunology. 2011; 55: 434-445. doi: 10.1111/j.1348-0421.2011.00334.x.
24.    Fan Y, Lu Y, Wang D, Liu J, et al. Effect of epimedium polysaccharide-propolis flavone immunopotentiator on immunosuppression induced by cyclophosphamide in chick¬ens. Cell Immunology. 2013; 281(1): 37-43. https://doi.org/10.1016/j.cellimm.2013.01.008
25.    Yu X, Wang C, Luo J, Zhao X, Wang L, Li X. Combination with methotrexate and cyclophosphamide attenuated maturation of dendritic cells: inducing Treg Skewing and Th17 suppression in vivo. Clinical and Developmental Immunology. 2013; 2013(1): 238035. doi: 10.1155/2013/238035.
26.    Yu Q, Nie SP, Wang JQ, Liu XZ, Yin PF, et al. Che¬moprotective effects of Ganoderma atrum polysaccharide in cyclophosphamide-induced mice. The International Journal of Biological Macromolecules. 2014; 64: 395–401. doi: 10.1016/j.ijbiomac.2013.12.029.
27.    Grnbaek H, Skjaerbaek C, Orskov H, Flyvbjerg A. Effect of immunosuppression on kidney and serum insulin-like growth factor-I (IGF-I), IGF binding proteins, and renal growth following unilateral nephrectomy in rats. Metabolism Clinical and Experimental, 1998; 47(7): 817-823. doi: 10.1016/s0026-0495(98)90119-6.
28.    Yu Q, Nie SP, Wang JQ, Liu XZ, Yin PF, et al. Che¬moprotective effects of Ganoderma atrum polysaccharide in cyclophosphamide-induced mice. International Journal of Biological Macromolecules. 2014; 64: 395-401.
29.    Elmadfa I, Klein P, Meyer AL. Immune-stimulating effects of lactic acid bacteria in vivo and in vitro. Proceedings of the Nutrition Society. 2010; 69(3): 416. doi: 10.1017/S0029665110001710.
30.    Masood MI, Qadir MI, Shirazi JH, Khan IU, et al. Beneficial ef¬fects of lactic acid bacteria on human beings. Critical Reviews in Microbiology. 2011; 37(1): 91. DOI:10.3109/1040841X.2010.536522
31.    Nakamura S, Kuda T, An C, Kanno T, Takahashi H, Kimura B. Inhibitory effects of Leuconostoc mesenteroides 1RM3 isolated from narezushi, a fermented fish with rice, on Listeria monocy¬togenes infection to Caco-2 cells and A/J mice. Anaerobe. 2012; 18(1): 19–24. doi: 10.1016/j.anaerobe.2011.11.006.
32.    Li CY, Lin HC, Lai CH, Lu JJ, Wu SF, Fang SH. Immunomodula¬tory effects of lactobacillus and Bifidobacterium on both murine and human mitogen-activated T cells. International Archives of Allergy and Immunology. 2011; 156(2): 128–36. doi: 10.1159/000322350.
33.    Li SP, Zhao XJ, Wang JY. Synergy of Astragalus polysaccha¬rides and probiotics (Lactobacillus and Bacillus cereus) on im¬munity and intestinal microbiota in chicks. Poultry Science. 2009; 88(3): 519–25.doi: 10.3382/ps.2008-00365.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags


Not Available