Author(s): Abdul Baquee Ahmed, Ripunjoy Bordoloi, Kunal Bhattacharya

Email(s): kunal22101994@gmail.com

DOI: 10.52711/0974-360X.2022.00129   

Address: Abdul Baquee Ahmed1, Ripunjoy Bordoloi2,3*, Kunal Bhattacharya3
1Girijananda Chowdhury Institute of Pharmaceutical Science, Tezpur, Assam – 784501.
2Assam Science and Technology University, Jalukbari, Guwahati, Assam-781013.
3NETES Institute of Pharmaceutical Science, Mirza, Kamrup, Assam-781125.
*Corresponding Author

Published In:   Volume - 15,      Issue - 2,     Year - 2022


ABSTRACT:
Objective: The objective of the current work is to develop and validate a simple yet effective, efficient and reproducible RP-UHPLC method for the determination of Gilcazide loaded in a microsphere formulation. Methods: The chromatographic analysis was carried out on Thermo Scientific (Dionex Ultra 3000plus) UHPLC using a stainless steel YMC C8 column (25cm × 4mm) packed with endcapped octylsilane bonded to porous silica (4µm) in isocratic elution mode using phosphate buffer pH 3.4 and HPLC grade acetonitrile in the ratio of 20: 80 (v/v) as eluent. The flow rate was adjusted as 1ml/min and eluent was detected at 230 nm. The retention time was observed at 1.9 min. Results: The linear dynamic range for the developed UHPLC method was found in the concentration range of 2-10µg/ml and percentage recovery was in the range of 94%-98%. Drug Entrapment Efficiency Data of Gliclazide loaded microsphere was found to be 93.72% Conclusion: In this RP–UHPLC method, the linearity was within the range of 2–10µg/mL and the validation parameters were within the limits was performed as per ICH guidelines. The % Drug entrapment Efficiency of the Gliclazide loaded microsphere was found to be 93.72 % which was satisfactory.


Cite this article:
Abdul Baquee Ahmed, Ripunjoy Bordoloi, Kunal Bhattacharya. Development and Validation of a RP-UHPLC Method for estimation of Gliclazide loaded Microsphere. Research Journal of Pharmacy and Technology. 2022; 15(2):773-8. doi: 10.52711/0974-360X.2022.00129

Cite(Electronic):
Abdul Baquee Ahmed, Ripunjoy Bordoloi, Kunal Bhattacharya. Development and Validation of a RP-UHPLC Method for estimation of Gliclazide loaded Microsphere. Research Journal of Pharmacy and Technology. 2022; 15(2):773-8. doi: 10.52711/0974-360X.2022.00129   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-2-49


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