Author(s):
Jayamini Jayantha, Banukie Jayasuriya, Dilanthi Herath, Sugandhika Suresh, Dhammika Magana-Arachchi
Email(s):
banukie@sjp.ac.lk
DOI:
10.52711/0974-360X.2022.00159 
Address:
Jayamini Jayantha1, Banukie Jayasuriya2*, Dilanthi Herath2, Sugandhika Suresh3, Dhammika Magana-Arachchi4
1Postgraduate Institute of Science, University of Peradeniya, Sri Lanka.
2Department of Pharmacy and Pharmaceutical Sciences, Faculty of Allied Health Sciences, University of Sri Jayewardenepura, Sri Lanka.
3Department of Biochemistry, Faculty of Medical Sciences, University of Sri Jayewardenepura, Sri Lanka.
4Molecular Microbiology and Human Diseases Project, National Institute of Fundamental Studies, Hantana Road, Kandy, Sri Lanka.
*Corresponding Author
Published In:
Volume - 15,
Issue - 3,
Year - 2022
ABSTRACT:
Tuberculosis (TB) is a chronic disease caused by Mycobacterium tuberculosis (Mtb) complex. The global TB epidemic has been aggravated by the emergence of disease outbreaks caused by multi-drug resistant and extensively drug-resistant strains. The aim of the present study was to evaluate the in-vitro, anti-TB activity of leaves of Psychotria sarmentosa, Aponogeton crispus and the mushrooms Pleurotus ostreatus and Pleurotus cystidiosus found in Sri Lanka. Leaves of Psychotria sarmentosa, Aponogeton crispus and the mushrooms; Pleurotus ostreatus and P. cystidiosus were dried until a constant weight and 120 g each were taken to prepare crude extracts with distilled water (1.9 L) by heating at a moderate temperature and the final volume was reduced to 240 ml. Freeze dried aqueous extracts were incorporated in Middle Brook 7H11 medium (1mg/ml) using pour plate method.Two ten-fold dilutions (10-2 and 10-4) of standard H37Rv Mtb suspensions were inoculated on Middle Brook 7H11 media with the crude extracts. The plates were incubated at 37 0C for 4 weeks until visible appearance of Mtb colonies. The inhibitory effect of each extract was calculated by the mean reduction of number of colonies on extract containing medium compared to extract-free control medium. Accordingly, the highest mean percentage inhibition was shown by P. sarmentosa (71.0 %). The mean percentage inhibition exerted by A. crispus, P. ostreatus and P. cystidiosus were 46.0 %, 43.4 % and 39.5 % respectively. Therefore, freeze-dried aqueous extract from leaves of P. sarmentosa has certain activity against the tested standard mycobacterial strain and has a potential to be used as an anti-TB drug component.
Cite this article:
Jayamini Jayantha, Banukie Jayasuriya, Dilanthi Herath, Sugandhika Suresh, Dhammika Magana-Arachchi. Determination of Anti-tuberculosis activity of Psychotria sarmentosa, Aponogeton crispus and two species of Pleurotus mushrooms. Research Journal of Pharmacy and Technology. 2022; 15(3):954-0. doi: 10.52711/0974-360X.2022.00159
Cite(Electronic):
Jayamini Jayantha, Banukie Jayasuriya, Dilanthi Herath, Sugandhika Suresh, Dhammika Magana-Arachchi. Determination of Anti-tuberculosis activity of Psychotria sarmentosa, Aponogeton crispus and two species of Pleurotus mushrooms. Research Journal of Pharmacy and Technology. 2022; 15(3):954-0. doi: 10.52711/0974-360X.2022.00159 Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-3-2
REFERENCES:
1. Anonymous.Global Tuberculosis Report. World health organization. 2020.
2. Tuyiringire et al. Three promising antimycobacterial medicinal plants reviewed as potential sources of drug hit candidates against multidrug-resistant tuberculosis. Tuberculosis. 2020;124: 101987.
3. Katalinic-Jankovic V, Furci L, Cirillo DM. Microbiology of mycobacterium tuberculosis and a new diagnostic test for TB. In European Respiratory Monograph, Edited by Hurst JR. European Respiratory Society, 2012; 8–13
4. Yew WW, Lange C, Leung CC. Treatment of tuberculosis: Update 2010. European Respiratory Journal. 2010; 37:441–62.
5. Chhipa et al. Nitroimidazoles: A newer class of Heterocycles for treatment of Tuberculosis. Research Journal of Pharmacy and Technology. 2020;13(7): 3425-32.
6. Hosseinzadeh et al. The application of medicinal plants in traditional and modern medicine: A Review of Thymus vulgaris. International Journal of Clinical Medicine. 2015; 6: 635-42.
7. Sanusi et al. Southeast Asian medicinal plants as a potential source of antituberculosis agent. Evidence-Based Complementary and Alternative Medicine. 2017; 39.
8. Ngoci et al. Bioactivity of Cissampelos pareira medicinal plant against Mycobacterium tuberculosis. Journal of Pharmacognosy and Phytochemistry. 2015; 3(6): 167-173.
9. Calixto et al. The Genus Psychotria: Phytochemistry, Chemotaxonomy, Ethnopharmacology and Biological Properties. Journal of the Brazilian Chemical Society. 2016; 27: 1355–78.
10. Chowdhury et al. Pharmacological values and phytochemical analysis of aquatic plant Genus Aponogeton: A review. International Journal of Recent Innovations in Academic Research. 2019; 3(3): 125-41.
11. Chowdhury NS, Farjana F, Sohrab H. Isolation, identification and pharmacological activities of endophytic fungi from Aponogeton undulatus Roxb. Pharmacology and Pharmacy, 2020; 11: 350-61.
12. Jayasuriya et al. Hypoglycaemic activity of culinary Pleurotus ostreatus and P. cystidiosus mushrooms in healthy volunteers and Type 2 diabetic patients on diet control and the possible mechanisms of action. Phytotherapy Research. 2015; 29: 303-09.
13. Jayasuriya et al. Anti-inflammatory activity of Pleurotus ostreatus, a culinary medicinal mushroom, in Wistar rats. Evidence Based Complementary and Alternative Medicine. 2020.
14. Chatterjee et al. Antimycobacterial activities of an edible mushroom extract and its synergy with the standard antituberculous drugs. Journal of Clinical Diagnostic Research. 2018; 12: 37-41.
15. Afieroho et al. Antituberculosis and phytochemical investigation of the dichloromethane extract Pleurotus Tuber-Regium (Fries) Singer Sclerotium. International Research Journal of Pharmacy. 2013; 4(1): 255–57.
16. Migliori et al. Drug resistance beyond extensively drug-resistant tuberculosis: individual patient data meta-analysis. European Respiratory Journal. 2013; 42: 169-179.
17. Aro et al. In vitro antimycobacterial, apoptosis-inducing potential, and immunomodulatory activity of some Rubiaceae Species. Frontiers in Pharmacology. 2019; 10: 1–13.
18. Ramos et al. Investigation of the antimycobacterial activity of 36 plant extracts from the Brazilian Atlantic forest. Brazilian Journal of Pharmaceutical Sciences. 2008; 44(4): 669–74.
19. Moraes et al. Antimycobacterial activity and alkaloid prospection of Psychotria species (Rubiaceae) from the Brazilian Atlantic Rainforest. Planta Medica. 2011; 77(9): 964–70.
20. Junior et al. Antimycobacterial and nitric oxide production inhibitory activities of triterpenes and alkaloids from Psychotria Nuda (Cham. & Schltdl.) Wawra. Molecules 2019; 24(6): 1–11.
21. Ratnayake, WMKM. Anti-inflammatory activities of Psychotria sarmentosa and Acroynchia pedunculata leaves. Ph.D. Thesis, University of Sri Jayewardenepura, Sri Lanka. 2018.
22. Teles et al. Effect of temperature on the degradation of bioactive compounds of Pinot Noir grape pomace during drying. Brazilian Journal of Food Technology. 2018; 21: e2017059.
23. Sandhya M and Disha K. Expension in the field of Freeze-drying: An Advanced Review. Research Journal of Pharmacy and Technology. 2020; 13(5): 2468-74.
24. Menikpurage IP, Soysa SSSBDP, Abeytunga DTU. Antioxidant activity and cytotoxicity of the edible mushroom, Pleurotus cystidiosus against hep-2 carcinoma cells. Journal of the National Foundation of Sri Lanka. 2012; 40(2): 106–13.
25. Sanchez JGB and Kouznetsov VV. Antimycobacterial susceptibility testing methods for natural products research. Brazilian Journal of Microbiology. 2010; 41: 270-77.
26. Famewo et al. Anti-mycobacterium tuberculosis activity of polyherbal medicines used for the treatment of tuberculosis in Eastern Cape, South Africa. African Health Sciences. 2017; 17(3): 780–89.
27. Betoni et al. Synergism between plant extract and antimicrobial drugs used on Staphylococcus aureus diseases. Memórias do Instituto Oswaldo Cruz. 2006; 101(4): 387-90.