Author(s): Jayamini Jayantha, Banukie Jayasuriya, Dilanthi Herath, Sugandhika Suresh, Dhammika Magana-Arachchi

Email(s): banukie@sjp.ac.lk

DOI: 10.52711/0974-360X.2022.00159   

Address: Jayamini Jayantha1, Banukie Jayasuriya2*, Dilanthi Herath2, Sugandhika Suresh3, Dhammika Magana-Arachchi4
1Postgraduate Institute of Science, University of Peradeniya, Sri Lanka.
2Department of Pharmacy and Pharmaceutical Sciences, Faculty of Allied Health Sciences, University of Sri Jayewardenepura, Sri Lanka.
3Department of Biochemistry, Faculty of Medical Sciences, University of Sri Jayewardenepura, Sri Lanka.
4Molecular Microbiology and Human Diseases Project, National Institute of Fundamental Studies, Hantana Road, Kandy, Sri Lanka.
*Corresponding Author

Published In:   Volume - 15,      Issue - 3,     Year - 2022


ABSTRACT:
Tuberculosis (TB) is a chronic disease caused by Mycobacterium tuberculosis (Mtb) complex. The global TB epidemic has been aggravated by the emergence of disease outbreaks caused by multi-drug resistant and extensively drug-resistant strains. The aim of the present study was to evaluate the in-vitro, anti-TB activity of leaves of Psychotria sarmentosa, Aponogeton crispus and the mushrooms Pleurotus ostreatus and Pleurotus cystidiosus found in Sri Lanka. Leaves of Psychotria sarmentosa, Aponogeton crispus and the mushrooms; Pleurotus ostreatus and P. cystidiosus were dried until a constant weight and 120 g each were taken to prepare crude extracts with distilled water (1.9 L) by heating at a moderate temperature and the final volume was reduced to 240 ml. Freeze dried aqueous extracts were incorporated in Middle Brook 7H11 medium (1mg/ml) using pour plate method.Two ten-fold dilutions (10-2 and 10-4) of standard H37Rv Mtb suspensions were inoculated on Middle Brook 7H11 media with the crude extracts. The plates were incubated at 37 0C for 4 weeks until visible appearance of Mtb colonies. The inhibitory effect of each extract was calculated by the mean reduction of number of colonies on extract containing medium compared to extract-free control medium. Accordingly, the highest mean percentage inhibition was shown by P. sarmentosa (71.0 %). The mean percentage inhibition exerted by A. crispus, P. ostreatus and P. cystidiosus were 46.0 %, 43.4 % and 39.5 % respectively. Therefore, freeze-dried aqueous extract from leaves of P. sarmentosa has certain activity against the tested standard mycobacterial strain and has a potential to be used as an anti-TB drug component.


Cite this article:
Jayamini Jayantha, Banukie Jayasuriya, Dilanthi Herath, Sugandhika Suresh, Dhammika Magana-Arachchi. Determination of Anti-tuberculosis activity of Psychotria sarmentosa, Aponogeton crispus and two species of Pleurotus mushrooms. Research Journal of Pharmacy and Technology. 2022; 15(3):954-0. doi: 10.52711/0974-360X.2022.00159

Cite(Electronic):
Jayamini Jayantha, Banukie Jayasuriya, Dilanthi Herath, Sugandhika Suresh, Dhammika Magana-Arachchi. Determination of Anti-tuberculosis activity of Psychotria sarmentosa, Aponogeton crispus and two species of Pleurotus mushrooms. Research Journal of Pharmacy and Technology. 2022; 15(3):954-0. doi: 10.52711/0974-360X.2022.00159   Available on: https://www.rjptonline.org/AbstractView.aspx?PID=2022-15-3-2


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